A practical enzymatic synthesis of UDP sugars and NDP glucoses

Jungdon Bae; Kwang Ho Kim; Dooil Kim; Yongseok Choi; Joong Su Kim; Sukhoon Koh; Suk In Hong; Dae Sil Lee

doi:10.1002/cbic.200500183

A practical enzymatic synthesis of UDP sugars and NDP glucoses

Jungdon Bae, Kwang Ho Kim, Dooil Kim, Yongseok Choi, Joong Su Kim, Sukhoon Koh, Suk In Hong, Dae Sil Lee

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

Mix and match. Nucleotide sugars are essential as glycoside donors to glycosyltransferases for the synthesis of bioactive metabolites. With 45 combinations of reactions with five NTPs and nine sugar-1-phosphates (Su1Ps) by recombinant UDP-sugar pyrophosphorylase (U_p) from Thermus caldophilus GK24, the enzyme showed broad substrate specificity toward five types of NTP with glucose-1-phosphate as well as four Su1Ps with UTP, affording all nine nucleotide sugars.

Original language	English
Pages (from-to)	1963-1966
Number of pages	4
Journal	ChemBioChem
Volume	6
Issue number	11
DOIs	https://doi.org/10.1002/cbic.200500183
Publication status	Published - 2005 Nov
Externally published	Yes

Keywords

Enzyme catalysis
Glycosyltransferases
Nucleotide sugars
Thermus caldophilus

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Organic Chemistry

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10.1002/cbic.200500183

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AU - Bae, Jungdon

AU - Kim, Kwang Ho

AU - Kim, Dooil

AU - Choi, Yongseok

AU - Kim, Joong Su

AU - Koh, Sukhoon

AU - Hong, Suk In

AU - Lee, Dae Sil

PY - 2005/11

Y1 - 2005/11

N2 - Mix and match. Nucleotide sugars are essential as glycoside donors to glycosyltransferases for the synthesis of bioactive metabolites. With 45 combinations of reactions with five NTPs and nine sugar-1-phosphates (Su1Ps) by recombinant UDP-sugar pyrophosphorylase (Up) from Thermus caldophilus GK24, the enzyme showed broad substrate specificity toward five types of NTP with glucose-1-phosphate as well as four Su1Ps with UTP, affording all nine nucleotide sugars.

AB - Mix and match. Nucleotide sugars are essential as glycoside donors to glycosyltransferases for the synthesis of bioactive metabolites. With 45 combinations of reactions with five NTPs and nine sugar-1-phosphates (Su1Ps) by recombinant UDP-sugar pyrophosphorylase (Up) from Thermus caldophilus GK24, the enzyme showed broad substrate specificity toward five types of NTP with glucose-1-phosphate as well as four Su1Ps with UTP, affording all nine nucleotide sugars.

KW - Enzyme catalysis

KW - Glycosyltransferases

KW - Nucleotide sugars

KW - Thermus caldophilus

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ER -

A practical enzymatic synthesis of UDP sugars and NDP glucoses

Abstract

Keywords

ASJC Scopus subject areas

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