A practical enzymatic synthesis of UDP sugars and NDP glucoses

Jungdon Bae; Kwang Ho Kim; Dooil Kim; Yongseok Choi; Joong Su Kim; Sukhoon Koh; Suk In Hong; Dae Sil Lee

doi:10.1002/cbic.200500183

A practical enzymatic synthesis of UDP sugars and NDP glucoses

Jungdon Bae
, Kwang Ho Kim
, Dooil Kim
, Yongseok Choi
, Joong Su Kim
, Sukhoon Koh
, Suk In Hong
, Dae Sil Lee^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

Mix and match. Nucleotide sugars are essential as glycoside donors to glycosyltransferases for the synthesis of bioactive metabolites. With 45 combinations of reactions with five NTPs and nine sugar-1-phosphates (Su1Ps) by recombinant UDP-sugar pyrophosphorylase (U_p) from Thermus caldophilus GK24, the enzyme showed broad substrate specificity toward five types of NTP with glucose-1-phosphate as well as four Su1Ps with UTP, affording all nine nucleotide sugars.

Original language	English
Pages (from-to)	1963-1966
Number of pages	4
Journal	ChemBioChem
Volume	6
Issue number	11
DOIs	https://doi.org/10.1002/cbic.200500183
Publication status	Published - 2005 Nov
Externally published	Yes

Keywords

Enzyme catalysis
Glycosyltransferases
Nucleotide sugars
Thermus caldophilus

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Organic Chemistry

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10.1002/cbic.200500183

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title = "A practical enzymatic synthesis of UDP sugars and NDP glucoses",

abstract = "Mix and match. Nucleotide sugars are essential as glycoside donors to glycosyltransferases for the synthesis of bioactive metabolites. With 45 combinations of reactions with five NTPs and nine sugar-1-phosphates (Su1Ps) by recombinant UDP-sugar pyrophosphorylase (Up) from Thermus caldophilus GK24, the enzyme showed broad substrate specificity toward five types of NTP with glucose-1-phosphate as well as four Su1Ps with UTP, affording all nine nucleotide sugars.",

keywords = "Enzyme catalysis, Glycosyltransferases, Nucleotide sugars, Thermus caldophilus",

author = "Jungdon Bae and Kim, \{Kwang Ho\} and Dooil Kim and Yongseok Choi and Kim, \{Joong Su\} and Sukhoon Koh and Hong, \{Suk In\} and Lee, \{Dae Sil\}",

year = "2005",

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doi = "10.1002/cbic.200500183",

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T1 - A practical enzymatic synthesis of UDP sugars and NDP glucoses

AU - Bae, Jungdon

AU - Kim, Kwang Ho

AU - Kim, Dooil

AU - Choi, Yongseok

AU - Kim, Joong Su

AU - Koh, Sukhoon

AU - Hong, Suk In

AU - Lee, Dae Sil

PY - 2005/11

Y1 - 2005/11

N2 - Mix and match. Nucleotide sugars are essential as glycoside donors to glycosyltransferases for the synthesis of bioactive metabolites. With 45 combinations of reactions with five NTPs and nine sugar-1-phosphates (Su1Ps) by recombinant UDP-sugar pyrophosphorylase (Up) from Thermus caldophilus GK24, the enzyme showed broad substrate specificity toward five types of NTP with glucose-1-phosphate as well as four Su1Ps with UTP, affording all nine nucleotide sugars.

AB - Mix and match. Nucleotide sugars are essential as glycoside donors to glycosyltransferases for the synthesis of bioactive metabolites. With 45 combinations of reactions with five NTPs and nine sugar-1-phosphates (Su1Ps) by recombinant UDP-sugar pyrophosphorylase (Up) from Thermus caldophilus GK24, the enzyme showed broad substrate specificity toward five types of NTP with glucose-1-phosphate as well as four Su1Ps with UTP, affording all nine nucleotide sugars.

KW - Enzyme catalysis

KW - Glycosyltransferases

KW - Nucleotide sugars

KW - Thermus caldophilus

UR - https://www.scopus.com/pages/publications/27744587503

U2 - 10.1002/cbic.200500183

DO - 10.1002/cbic.200500183

M3 - Article

C2 - 16206230

AN - SCOPUS:27744587503

SN - 1439-4227

VL - 6

SP - 1963

EP - 1966

JO - ChemBioChem

JF - ChemBioChem

IS - 11

ER -

A practical enzymatic synthesis of UDP sugars and NDP glucoses

Abstract

Keywords

ASJC Scopus subject areas

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