TY - JOUR
T1 - A pre-clinical assessment model of rat autogeneic bone marrow stromal cell transplantation into the central nervous system
AU - Lee, Jang Bo
AU - Kuroda, Satoshi
AU - Shichinohe, Hideo
AU - Yano, Shunsuke
AU - Kobayashi, Hiroyuki
AU - Hida, Kazutoshi
AU - Iwasaki, Yoshinobu
N1 - Funding Information:
The present study was supported by Grant-in-Aids from the Ministry of Education, Science, and Culture of Japan (No. 14370424, Dr. Kuroda and No. 15390426, Dr. Iwasaki), and by a grant from the Mitsubishi Pharma Research Foundation (Dr. Kuroda). The authors thank Ms. Yumiko Shinohe for her technical assistance in cell culture and immunohistochemistry.
PY - 2004/11
Y1 - 2004/11
N2 - In order to verify the biological aspects of 'autogeneic' bone marrow stromal cells (BMSC) transplantation for neurological disorders, we aimed our study towards the assessment of the survival, distribution, and differentiation of autologous BMSC in the central nervous system (CNS). We harvested rat BMSC from femur bones, and the nuclei were then fluorescently labeled by a 24-h co-culture with bis-benzimide. These BMSC were stereotactically injected into the striatum (n=6) or thoracic cord (n=8) of each animal. We evaluated the distribution and differentiation of 'autogeneic' BMSC in the brain and spinal cord after 4 weeks, using the immunohistochemistry technique. We found some injected cells in the ipsilateral striatum, hippocampus, neocortex, and bilateral corpus callosum, and approximately 20% and 15% of the engrafted cells expressed neuronal and astrocytic markers, respectively. Other injected cells were distributed in the dorsal funiculus and adjacent gray matter, and about 10% and 15% of these cells expressed neuronal and astrocytic markers, respectively. Although the precise mechanism of BMSC transdifferentiation still remains unclear, the present results show that 'autogeneic' BMSC could highly differentiate into their own CNS neural cells, suggesting that they are surrounded by favorable conditions.
AB - In order to verify the biological aspects of 'autogeneic' bone marrow stromal cells (BMSC) transplantation for neurological disorders, we aimed our study towards the assessment of the survival, distribution, and differentiation of autologous BMSC in the central nervous system (CNS). We harvested rat BMSC from femur bones, and the nuclei were then fluorescently labeled by a 24-h co-culture with bis-benzimide. These BMSC were stereotactically injected into the striatum (n=6) or thoracic cord (n=8) of each animal. We evaluated the distribution and differentiation of 'autogeneic' BMSC in the brain and spinal cord after 4 weeks, using the immunohistochemistry technique. We found some injected cells in the ipsilateral striatum, hippocampus, neocortex, and bilateral corpus callosum, and approximately 20% and 15% of the engrafted cells expressed neuronal and astrocytic markers, respectively. Other injected cells were distributed in the dorsal funiculus and adjacent gray matter, and about 10% and 15% of these cells expressed neuronal and astrocytic markers, respectively. Although the precise mechanism of BMSC transdifferentiation still remains unclear, the present results show that 'autogeneic' BMSC could highly differentiate into their own CNS neural cells, suggesting that they are surrounded by favorable conditions.
KW - Bone marrow stromal cell
KW - Differentiation
KW - Microenvironment
KW - Migration
KW - Transplantation, autograft
KW - bis-Benzimide
UR - http://www.scopus.com/inward/record.url?scp=7444264696&partnerID=8YFLogxK
U2 - 10.1016/j.brainresprot.2004.09.004
DO - 10.1016/j.brainresprot.2004.09.004
M3 - Article
C2 - 15519950
AN - SCOPUS:7444264696
SN - 1385-299X
VL - 14
SP - 37
EP - 44
JO - Brain Research Protocols
JF - Brain Research Protocols
IS - 1
ER -