Abstract
Phylogenetic analysis of the ITS rDNA region was carried out with two economically important downy mildews, Pseudoperonospora cubensis, which infects species of Cucumis, Cucurbita, and Citrullus belonging to Cucurbitaceae, and P. humuli, which infects plants of the genus Humulus belonging to Cannabaceae. Two closely related species, P. cannabina and P. celtidis, were also included to reveal taxonomic relationships with the first two mildews. All four species formed a well-resolved clade when compared with the ITS sequences of other downy mildew genera, using Bayesian inference and maximum parsimony. The P. cubensis isolates obtained from different hosts and (or) geographical origins in Korea, exhibited no intraspecific variability in the ITS sequences. The phylogenetic analyses of P. cubensis and P. humuli showed that they share a high level of sequence homology; the morphology of the sporangiophores, sporangia, and dehiscence apparatus confirmed the similarity of the two species. We therefore reduce P. humuli to the status of a taxonomic synonym of P. cubensis.
| Original language | English |
|---|---|
| Pages (from-to) | 841-848 |
| Number of pages | 8 |
| Journal | Mycological Research |
| Volume | 109 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 2005 Jul |
Bibliographical note
Funding Information:We are grateful to Ovidiu Constantinescu (Uppsala University), for helpful comments and suggestions, to the curators of the Moravian Museum, (Czech Republic), Mycological Collection of Institute of Botany (Bulgarian Academy of Sciences), and of mycological collection of the Jardin Botanique National de Belgique for providing Pseudoperonospora specimens; and to the staff of the Korean Agricultural Culture Collection for technical assistance in DNA sequencing and phylogenetic analysis. This work was financially supported by a Korea University grant in 2004.
ASJC Scopus subject areas
- Biotechnology
- Ecology, Evolution, Behavior and Systematics
- Genetics
- Plant Science