TY - JOUR
T1 - A re-consideration of Pseudoperonospora cubensis and P. humuli based on molecular and morphological data
AU - Choi, Young Joon
AU - Hong, Seung Beom
AU - Shin, Hyeon Dong
N1 - Funding Information:
We are grateful to Ovidiu Constantinescu (Uppsala University), for helpful comments and suggestions, to the curators of the Moravian Museum, (Czech Republic), Mycological Collection of Institute of Botany (Bulgarian Academy of Sciences), and of mycological collection of the Jardin Botanique National de Belgique for providing Pseudoperonospora specimens; and to the staff of the Korean Agricultural Culture Collection for technical assistance in DNA sequencing and phylogenetic analysis. This work was financially supported by a Korea University grant in 2004.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2005/7
Y1 - 2005/7
N2 - Phylogenetic analysis of the ITS rDNA region was carried out with two economically important downy mildews, Pseudoperonospora cubensis, which infects species of Cucumis, Cucurbita, and Citrullus belonging to Cucurbitaceae, and P. humuli, which infects plants of the genus Humulus belonging to Cannabaceae. Two closely related species, P. cannabina and P. celtidis, were also included to reveal taxonomic relationships with the first two mildews. All four species formed a well-resolved clade when compared with the ITS sequences of other downy mildew genera, using Bayesian inference and maximum parsimony. The P. cubensis isolates obtained from different hosts and (or) geographical origins in Korea, exhibited no intraspecific variability in the ITS sequences. The phylogenetic analyses of P. cubensis and P. humuli showed that they share a high level of sequence homology; the morphology of the sporangiophores, sporangia, and dehiscence apparatus confirmed the similarity of the two species. We therefore reduce P. humuli to the status of a taxonomic synonym of P. cubensis.
AB - Phylogenetic analysis of the ITS rDNA region was carried out with two economically important downy mildews, Pseudoperonospora cubensis, which infects species of Cucumis, Cucurbita, and Citrullus belonging to Cucurbitaceae, and P. humuli, which infects plants of the genus Humulus belonging to Cannabaceae. Two closely related species, P. cannabina and P. celtidis, were also included to reveal taxonomic relationships with the first two mildews. All four species formed a well-resolved clade when compared with the ITS sequences of other downy mildew genera, using Bayesian inference and maximum parsimony. The P. cubensis isolates obtained from different hosts and (or) geographical origins in Korea, exhibited no intraspecific variability in the ITS sequences. The phylogenetic analyses of P. cubensis and P. humuli showed that they share a high level of sequence homology; the morphology of the sporangiophores, sporangia, and dehiscence apparatus confirmed the similarity of the two species. We therefore reduce P. humuli to the status of a taxonomic synonym of P. cubensis.
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U2 - 10.1017/S0953756205002534
DO - 10.1017/S0953756205002534
M3 - Article
C2 - 16121571
AN - SCOPUS:23744441677
SN - 1878-6146
VL - 109
SP - 841
EP - 848
JO - Fungal Biology
JF - Fungal Biology
IS - 7
ER -