A Ribosome–Inactivating Protein from Amaranthus viridis >)

Seok Yoon Kwon; Chung Sun An; Jang Ryol Liu; Kyung Hee Paek

doi:10.1271/bbb.61.1613

A Ribosome–Inactivating Protein from Amaranthus viridis >)

Seok Yoon Kwon, Chung Sun An, Jang Ryol Liu, Kyung Hee Paek

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

Abstract

An antiviral protein purified from the leaves of Amaranthus viridis was named amaranthin. The in vivo antiviral activity of amaranthin was confirmed in tobacco mosaic virus (TMV) infection test on Nicotiana glutinosa leaves. The molecular mass of the amaranthin was estimated about 30 kDa by SDS–PAGE and the pI was measured as 9.8 by isoelectric focusing (IEF) analysis. Cytotoxicity of the amaranthin using in vitro translation inhibition assay was similar to that of pokeweed antiviral protein (PAP) with IC₅₀ of 25pM. Depurination activity (N-glycosidase activity) against animal rRNA was also confirmed.

Original language	English
Pages (from-to)	1613-1614
Number of pages	2
Journal	Bioscience, Biotechnology and Biochemistry
Volume	61
Issue number	9
DOIs	https://doi.org/10.1271/bbb.61.1613
Publication status	Published - 1997 Jan

Bibliographical note

Funding Information:
Acknowledgments. This study was supported by grants from the Korean Ministry of Science and Technology through the HAN project and the Korean Science and Engineering Foundation through RRC.

Keywords

Amaranthus viridis
Antiviral protein
In vitro translation
RIP
TMV

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

Access to Document

10.1271/bbb.61.1613

Cite this

@article{f32b6f0160504fe49f0d0448ec3062f7,

title = "A Ribosome–Inactivating Protein from Amaranthus viridis >)",

abstract = "An antiviral protein purified from the leaves of Amaranthus viridis was named amaranthin. The in vivo antiviral activity of amaranthin was confirmed in tobacco mosaic virus (TMV) infection test on Nicotiana glutinosa leaves. The molecular mass of the amaranthin was estimated about 30 kDa by SDS–PAGE and the pI was measured as 9.8 by isoelectric focusing (IEF) analysis. Cytotoxicity of the amaranthin using in vitro translation inhibition assay was similar to that of pokeweed antiviral protein (PAP) with IC50 of 25pM. Depurination activity (N-glycosidase activity) against animal rRNA was also confirmed.",

keywords = "Amaranthus viridis, Antiviral protein, In vitro translation, RIP, TMV",

author = "Kwon, {Seok Yoon} and An, {Chung Sun} and Liu, {Jang Ryol} and Paek, {Kyung Hee}",

note = "Funding Information: Acknowledgments. This study was supported by grants from the Korean Ministry of Science and Technology through the HAN project and the Korean Science and Engineering Foundation through RRC.",

year = "1997",

month = jan,

doi = "10.1271/bbb.61.1613",

language = "English",

volume = "61",

pages = "1613--1614",

journal = "Bioscience, Biotechnology and Biochemistry",

issn = "0916-8451",

publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",

number = "9",

}

TY - JOUR

T1 - A Ribosome–Inactivating Protein from Amaranthus viridis >)

AU - Kwon, Seok Yoon

AU - An, Chung Sun

AU - Liu, Jang Ryol

AU - Paek, Kyung Hee

N1 - Funding Information: Acknowledgments. This study was supported by grants from the Korean Ministry of Science and Technology through the HAN project and the Korean Science and Engineering Foundation through RRC.

PY - 1997/1

Y1 - 1997/1

N2 - An antiviral protein purified from the leaves of Amaranthus viridis was named amaranthin. The in vivo antiviral activity of amaranthin was confirmed in tobacco mosaic virus (TMV) infection test on Nicotiana glutinosa leaves. The molecular mass of the amaranthin was estimated about 30 kDa by SDS–PAGE and the pI was measured as 9.8 by isoelectric focusing (IEF) analysis. Cytotoxicity of the amaranthin using in vitro translation inhibition assay was similar to that of pokeweed antiviral protein (PAP) with IC50 of 25pM. Depurination activity (N-glycosidase activity) against animal rRNA was also confirmed.

AB - An antiviral protein purified from the leaves of Amaranthus viridis was named amaranthin. The in vivo antiviral activity of amaranthin was confirmed in tobacco mosaic virus (TMV) infection test on Nicotiana glutinosa leaves. The molecular mass of the amaranthin was estimated about 30 kDa by SDS–PAGE and the pI was measured as 9.8 by isoelectric focusing (IEF) analysis. Cytotoxicity of the amaranthin using in vitro translation inhibition assay was similar to that of pokeweed antiviral protein (PAP) with IC50 of 25pM. Depurination activity (N-glycosidase activity) against animal rRNA was also confirmed.

KW - Amaranthus viridis

KW - Antiviral protein

KW - In vitro translation

KW - RIP

KW - TMV

UR - http://www.scopus.com/inward/record.url?scp=0031216718&partnerID=8YFLogxK

U2 - 10.1271/bbb.61.1613

DO - 10.1271/bbb.61.1613

M3 - Article

C2 - 9339569

AN - SCOPUS:0031216718

SN - 0916-8451

VL - 61

SP - 1613

EP - 1614

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 9

ER -

A Ribosome–Inactivating Protein from Amaranthus viridis >)

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this