A self-calibrating bipartite viscosity sensor for mitochondria

Zhigang Yang; Yanxia He; Jae Hong Lee; Nayoung Park; Myungkoo Suh; Weon Sik Chae; Jianfang Cao; Xiaojun Peng; Hyosung Jung; Chulhun Kang; Jong Seung Kim

doi:10.1021/ja403851p

A self-calibrating bipartite viscosity sensor for mitochondria

Zhigang Yang, Yanxia He, Jae Hong Lee, Nayoung Park, Myungkoo Suh, Weon Sik Chae, Jianfang Cao, Xiaojun Peng, Hyosung Jung, Chulhun Kang, Jong Seung Kim

Research output: Contribution to journal › Article › peer-review

398 Citations (Scopus)

Abstract

A self-calibrating bipartite viscosity sensor 1 for cellular mitochondria, composed of coumarin and boron-dipyrromethene (BODIPY) with a rigid phenyl spacer and a mitochondria-targeting unit, was synthesized. The sensor showed a direct linear relationship between the fluorescence intensity ratio of BODIPY to coumarin or the fluorescence lifetime ratio and the media viscosity, which allowed us to determine the average mitochondrial viscosity in living HeLa cells as ca. 62 cP (cp). Upon treatment with an ionophore, monensin, or nystatin, the mitochondrial viscosity was observed to increase to ca. 110 cP.

Original language	English
Pages (from-to)	9181-9185
Number of pages	5
Journal	Journal of the American Chemical Society
Volume	135
Issue number	24
DOIs	https://doi.org/10.1021/ja403851p
Publication status	Published - 2013 Jun 19

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

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abstract = "A self-calibrating bipartite viscosity sensor 1 for cellular mitochondria, composed of coumarin and boron-dipyrromethene (BODIPY) with a rigid phenyl spacer and a mitochondria-targeting unit, was synthesized. The sensor showed a direct linear relationship between the fluorescence intensity ratio of BODIPY to coumarin or the fluorescence lifetime ratio and the media viscosity, which allowed us to determine the average mitochondrial viscosity in living HeLa cells as ca. 62 cP (cp). Upon treatment with an ionophore, monensin, or nystatin, the mitochondrial viscosity was observed to increase to ca. 110 cP.",

author = "Zhigang Yang and Yanxia He and Lee, {Jae Hong} and Nayoung Park and Myungkoo Suh and Chae, {Weon Sik} and Jianfang Cao and Xiaojun Peng and Hyosung Jung and Chulhun Kang and Kim, {Jong Seung}",

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AU - Yang, Zhigang

AU - He, Yanxia

AU - Lee, Jae Hong

AU - Park, Nayoung

AU - Suh, Myungkoo

AU - Chae, Weon Sik

AU - Cao, Jianfang

AU - Peng, Xiaojun

AU - Jung, Hyosung

AU - Kang, Chulhun

AU - Kim, Jong Seung

PY - 2013/6/19

Y1 - 2013/6/19

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AB - A self-calibrating bipartite viscosity sensor 1 for cellular mitochondria, composed of coumarin and boron-dipyrromethene (BODIPY) with a rigid phenyl spacer and a mitochondria-targeting unit, was synthesized. The sensor showed a direct linear relationship between the fluorescence intensity ratio of BODIPY to coumarin or the fluorescence lifetime ratio and the media viscosity, which allowed us to determine the average mitochondrial viscosity in living HeLa cells as ca. 62 cP (cp). Upon treatment with an ionophore, monensin, or nystatin, the mitochondrial viscosity was observed to increase to ca. 110 cP.

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A self-calibrating bipartite viscosity sensor for mitochondria

Abstract

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