A self-calibrating bipartite viscosity sensor for mitochondria

Zhigang Yang, Yanxia He, Jae Hong Lee, Nayoung Park, Myungkoo Suh, Weon Sik Chae, Jianfang Cao, Xiaojun Peng, Hyosung Jung, Chulhun Kang, Jong Seung Kim

Research output: Contribution to journalArticlepeer-review

359 Citations (Scopus)


A self-calibrating bipartite viscosity sensor 1 for cellular mitochondria, composed of coumarin and boron-dipyrromethene (BODIPY) with a rigid phenyl spacer and a mitochondria-targeting unit, was synthesized. The sensor showed a direct linear relationship between the fluorescence intensity ratio of BODIPY to coumarin or the fluorescence lifetime ratio and the media viscosity, which allowed us to determine the average mitochondrial viscosity in living HeLa cells as ca. 62 cP (cp). Upon treatment with an ionophore, monensin, or nystatin, the mitochondrial viscosity was observed to increase to ca. 110 cP.

Original languageEnglish
Pages (from-to)9181-9185
Number of pages5
JournalJournal of the American Chemical Society
Issue number24
Publication statusPublished - 2013 Jun 19

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry


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