A self-calibrating bipartite viscosity sensor for mitochondria

Zhigang Yang, Yanxia He, Jae Hong Lee, Nayoung Park, Myungkoo Suh, Weon Sik Chae, Jianfang Cao, Xiaojun Peng, Hyosung Jung, Chulhun Kang, Jong Seung Kim

    Research output: Contribution to journalArticlepeer-review

    407 Citations (Scopus)

    Abstract

    A self-calibrating bipartite viscosity sensor 1 for cellular mitochondria, composed of coumarin and boron-dipyrromethene (BODIPY) with a rigid phenyl spacer and a mitochondria-targeting unit, was synthesized. The sensor showed a direct linear relationship between the fluorescence intensity ratio of BODIPY to coumarin or the fluorescence lifetime ratio and the media viscosity, which allowed us to determine the average mitochondrial viscosity in living HeLa cells as ca. 62 cP (cp). Upon treatment with an ionophore, monensin, or nystatin, the mitochondrial viscosity was observed to increase to ca. 110 cP.

    Original languageEnglish
    Pages (from-to)9181-9185
    Number of pages5
    JournalJournal of the American Chemical Society
    Volume135
    Issue number24
    DOIs
    Publication statusPublished - 2013 Jun 19

    ASJC Scopus subject areas

    • Catalysis
    • General Chemistry
    • Biochemistry
    • Colloid and Surface Chemistry

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