A sensitive and reliable detection of thrombin via enzyme-precipitate-coating-linked aptamer assay

Hye Jin Lee; Byoung Chan Kim; Min Kyu Oh; Jungbae Kim

doi:10.1039/c2cc30710c

A sensitive and reliable detection of thrombin via enzyme-precipitate-coating-linked aptamer assay

Hye Jin Lee, Byoung Chan Kim, Min Kyu Oh, Jungbae Kim

Department of Chemical and Biological Engineering

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Stable enzyme precipitate coatings (EPCs) with high enzyme loading were employed to develop a sensitive and reliable detection protocol, termed EPC-linked aptamer assay (EPC-LAA). EPC-LAA achieved the limit of detection to be 0.5 ng per mL of thrombin (13.5 pM), and maintained a reasonably stable detection signal even after storing all the reagents at 40 °C for 66 days.

Original language	English
Pages (from-to)	5971-5973
Number of pages	3
Journal	Chemical Communications
Volume	48
Issue number	48
DOIs	https://doi.org/10.1039/c2cc30710c
Publication status	Published - 2012 May 21

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
General Chemistry
Ceramics and Composites
Metals and Alloys
Materials Chemistry
Surfaces, Coatings and Films
Catalysis

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abstract = "Stable enzyme precipitate coatings (EPCs) with high enzyme loading were employed to develop a sensitive and reliable detection protocol, termed EPC-linked aptamer assay (EPC-LAA). EPC-LAA achieved the limit of detection to be 0.5 ng per mL of thrombin (13.5 pM), and maintained a reasonably stable detection signal even after storing all the reagents at 40 °C for 66 days.",

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AU - Kim, Byoung Chan

AU - Oh, Min Kyu

AU - Kim, Jungbae

PY - 2012/5/21

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AB - Stable enzyme precipitate coatings (EPCs) with high enzyme loading were employed to develop a sensitive and reliable detection protocol, termed EPC-linked aptamer assay (EPC-LAA). EPC-LAA achieved the limit of detection to be 0.5 ng per mL of thrombin (13.5 pM), and maintained a reasonably stable detection signal even after storing all the reagents at 40 °C for 66 days.

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A sensitive and reliable detection of thrombin via enzyme-precipitate-coating-linked aptamer assay

Abstract

ASJC Scopus subject areas

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