A simple and rapid method for isolation of high quality genomic DNA from fruit trees and conifers using PVP

C. S. Kim; C. H. Lee; J. S. Shin; Y. S. Chung; N. I. Hyung

doi:10.1093/nar/25.5.1085

A simple and rapid method for isolation of high quality genomic DNA from fruit trees and conifers using PVP

C. S. Kim, C. H. Lee, J. S. Shin, Y. S. Chung, N. I. Hyung

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

141 Citations (Scopus)

Abstract

Because DNA degradation is mediated by secondary plant products such as phenolic terpenoids, the isolation of high quality DNA from plants containing a high content of polyphenolics has been a difficult problem. We demonstrate an easy extraction process by modifying several existing ones. Using this process we have found it possible to isolate DNAs from four fruit trees, grape (Vitis spp.), apple (Malus spp.), pear (Pyrus spp.) and persimmon (Diospyros spp.) and four species of conifer, Pinus densiflora, Pinus koraiensis, Taxus cuspidata and Juniperus chinensis within a few hours. Compared with the existing method, we have isolated high quality intact DNAs (260/280 = 1.8-2.0) routinely yielding 250-500 ng/μl (total 7.5-15 μg DNA from four to five tissue discs).

Original language	English
Pages (from-to)	1085-1086
Number of pages	2
Journal	Nucleic acids research
Volume	25
Issue number	5
DOIs	https://doi.org/10.1093/nar/25.5.1085
Publication status	Published - 1996 Sept 5

ASJC Scopus subject areas

Genetics

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abstract = "Because DNA degradation is mediated by secondary plant products such as phenolic terpenoids, the isolation of high quality DNA from plants containing a high content of polyphenolics has been a difficult problem. We demonstrate an easy extraction process by modifying several existing ones. Using this process we have found it possible to isolate DNAs from four fruit trees, grape (Vitis spp.), apple (Malus spp.), pear (Pyrus spp.) and persimmon (Diospyros spp.) and four species of conifer, Pinus densiflora, Pinus koraiensis, Taxus cuspidata and Juniperus chinensis within a few hours. Compared with the existing method, we have isolated high quality intact DNAs (260/280 = 1.8-2.0) routinely yielding 250-500 ng/μl (total 7.5-15 μg DNA from four to five tissue discs).",

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AU - Kim, C. S.

AU - Lee, C. H.

AU - Shin, J. S.

AU - Chung, Y. S.

AU - Hyung, N. I.

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AB - Because DNA degradation is mediated by secondary plant products such as phenolic terpenoids, the isolation of high quality DNA from plants containing a high content of polyphenolics has been a difficult problem. We demonstrate an easy extraction process by modifying several existing ones. Using this process we have found it possible to isolate DNAs from four fruit trees, grape (Vitis spp.), apple (Malus spp.), pear (Pyrus spp.) and persimmon (Diospyros spp.) and four species of conifer, Pinus densiflora, Pinus koraiensis, Taxus cuspidata and Juniperus chinensis within a few hours. Compared with the existing method, we have isolated high quality intact DNAs (260/280 = 1.8-2.0) routinely yielding 250-500 ng/μl (total 7.5-15 μg DNA from four to five tissue discs).

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A simple and rapid method for isolation of high quality genomic DNA from fruit trees and conifers using PVP

Abstract

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