A simple and reliable method for obtaining Entomosporium monoconidial isolates

Mi Jeong Park; Quinn A. Holtslag; Hyeon Dong Shin

doi:10.1007/s12275-011-1117-y

A simple and reliable method for obtaining Entomosporium monoconidial isolates

Mi Jeong Park, Quinn A. Holtslag, Hyeon Dong Shin

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Monoconidial isolates of Entomosporium mespili were successfully cultured using a simple isolation procedure. A detailed description of the steps required for isolating E. mespili is provided. The characteristic pattern of conidial germination and growth on potato dextrose agar is also described. The process that was successful in obtaining pure isolates involved collecting material in the morning, picking a glistening white mass of conidia under dissecting microscope magnification, depositing a mass of conidia onto a drop of water on a glass slide, streaking the conidial mass onto potato dextrose agar, incubating the plate for 48 h, and transferring the characteristic fan-shaped colonies to fresh medium.

Original language	English
Pages (from-to)	324-326
Number of pages	3
Journal	Journal of Microbiology
Volume	49
Issue number	2
DOIs	https://doi.org/10.1007/s12275-011-1117-y
Publication status	Published - 2011 Apr

Bibliographical note

Funding Information:
This study was supported in part by a grant from Regional Subgenebank Support Program of Rural Development Administration, Republic of Korea.

Keywords

Entomosporium mespili
fan-shaped colony
germination
isolation technique

ASJC Scopus subject areas

Microbiology
Applied Microbiology and Biotechnology

Access to Document

10.1007/s12275-011-1117-y

Cite this

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title = "A simple and reliable method for obtaining Entomosporium monoconidial isolates",

abstract = "Monoconidial isolates of Entomosporium mespili were successfully cultured using a simple isolation procedure. A detailed description of the steps required for isolating E. mespili is provided. The characteristic pattern of conidial germination and growth on potato dextrose agar is also described. The process that was successful in obtaining pure isolates involved collecting material in the morning, picking a glistening white mass of conidia under dissecting microscope magnification, depositing a mass of conidia onto a drop of water on a glass slide, streaking the conidial mass onto potato dextrose agar, incubating the plate for 48 h, and transferring the characteristic fan-shaped colonies to fresh medium.",

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note = "Funding Information: This study was supported in part by a grant from Regional Subgenebank Support Program of Rural Development Administration, Republic of Korea.",

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doi = "10.1007/s12275-011-1117-y",

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AU - Holtslag, Quinn A.

AU - Shin, Hyeon Dong

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AB - Monoconidial isolates of Entomosporium mespili were successfully cultured using a simple isolation procedure. A detailed description of the steps required for isolating E. mespili is provided. The characteristic pattern of conidial germination and growth on potato dextrose agar is also described. The process that was successful in obtaining pure isolates involved collecting material in the morning, picking a glistening white mass of conidia under dissecting microscope magnification, depositing a mass of conidia onto a drop of water on a glass slide, streaking the conidial mass onto potato dextrose agar, incubating the plate for 48 h, and transferring the characteristic fan-shaped colonies to fresh medium.

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A simple and reliable method for obtaining Entomosporium monoconidial isolates

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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