Abstract
We report a new and simple design of a fully automated dual-online ultra-high pressure liquid chromatography system. The system employs only two nano-volume switching valves (a two-position four port valve and a two-position ten port valve) that direct solvent flows from two binary nano-pumps for parallel operation of two analytical columns and two solid phase extraction (SPE) columns. Despite the simple design, the sDO-UHPLC offers many advantageous features that include high duty cycle, back flushing sample injection for fast and narrow zone sample injection, online desalting, high separation resolution and high intra/inter-column reproducibility. This system was applied to analyze proteome samples not only in high throughput deep proteome profiling experiments but also in high throughput MRM experiments.
| Original language | English |
|---|---|
| Pages (from-to) | 5700-5706 |
| Number of pages | 7 |
| Journal | Analyst |
| Volume | 140 |
| Issue number | 16 |
| DOIs | |
| Publication status | Published - 2015 Aug 21 |
Bibliographical note
Publisher Copyright:© The Royal Society of Chemistry 2015.
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Environmental Chemistry
- Spectroscopy
- Electrochemistry