A simple method for activating the platelets used in microfluidic platelet aggregation tests: Stirring-induced platelet activation

Hoyoon Lee, Gyehyu Kim, Chaeseung Lim, Byoung Kwon Lee, Sehyun Shin

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)


High-shear stimulation is well known as one of the key factors affecting platelet activation and aggregation, which can lead to the formation of a thrombus. In one of our previous studies, we introduced migration distance-based platelet function analysis in a microfluidic system. In this study, we set out to examine the effects of stirring on shear-induced platelet activation and aggregation in a chamber system by using a rotating stirrer. We found that the rotating stirrer caused not only rotational shear flow but also a strong radial secondary flow. The latter flow led to efficient mixing in the chamber. Moreover, the rotational flow led to the generation of shear stress, the magnitude of which can be controlled to activate the platelets. Activated platelets tend to aggregate themselves. The maximum platelet aggregation was observed at a critical shear rate of 3100 s-1, regardless of the stirrer shape. Furthermore, the time taken to attain maximum aggregation was significantly shortened when using a wide stirrer (30 s) instead of a narrow one (180 s). When using a flat stirrer, the non-uniform shear field in the chamber system was resolved with the radial secondary flow-induced mixing; thus, most of the platelets were homogenously activated. The stirring-induced platelet activation mechanism was experimentally confirmed in a microfluidic system for a platelet aggregation test while monitoring the migration distance until the microfluidic channel is occluded. Our findings indicate that the present system, consisting of a rotating stirrer and a confined chamber, provides effective shear stimulation for activating platelets and inducing platelet aggregates.

Original languageEnglish
Article number064118
Issue number6
Publication statusPublished - 2016 Nov 1

Bibliographical note

Publisher Copyright:
© 2016 Author(s).

ASJC Scopus subject areas

  • Biomedical Engineering
  • General Materials Science
  • Condensed Matter Physics
  • Fluid Flow and Transfer Processes
  • Colloid and Surface Chemistry


Dive into the research topics of 'A simple method for activating the platelets used in microfluidic platelet aggregation tests: Stirring-induced platelet activation'. Together they form a unique fingerprint.

Cite this