A simple morphometric analysis method for dermal microstructure using color thresholding and moments

Ji Hyuck Hong; Dai Hyun Kim; Im Joo Rhyu; Young Chul Kye; Hyo Hyun Ahn

doi:10.1111/srt.12776

A simple morphometric analysis method for dermal microstructure using color thresholding and moments

Ji Hyuck Hong, Dai Hyun Kim, Im Joo Rhyu, Young Chul Kye, Hyo Hyun Ahn

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

Background: Proper assessment of dermal collagen fibers by dermatologists and researchers is essential. Histologic evaluation methods have limitations. We present a simple method for measurement of collagen fibers in human skin using Masson's trichrome staining. Materials and Methods: Normal skin specimens from a cadaver were processed with Masson's trichrome, which can effectively stain collagen fibers blue with aniline dye. Optical photomicrographs of these slides were analyzed using ImageJ software. Color image processing, a histogram-based function of ImageJ for image segmentation, was performed with color moments thresholding technique. We selected blue areas by adjusting the blue channel to include specific values. The selected areas were highlighted and evaluated. We divided the image into layers of 0.09-mm² areas from the top to bottom of the dermis. Each area was cropped and evaluated. Results: Quantitative assessment yielded the quantitative size occupied by collagen fibers in an area of 0.09 mm². Calculation of the percentage in each area can be used to determine the density of collagen fibers. Conclusion: Measurements obtained with our method can be applied to research on dermal collagen fibers. We present a convenient quantitative assessment method for the dermal constituents in Masson's trichrome-stained slides.

Original language	English
Pages (from-to)	132-136
Number of pages	5
Journal	Skin Research and Technology
Volume	26
Issue number	1
DOIs	https://doi.org/10.1111/srt.12776
Publication status	Published - 2020 Jan 1

Bibliographical note

Funding Information:
This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Science, ICT & Future Planning (NRF‐2017R1A2B4011001).

Publisher Copyright:
© 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd

Keywords

Histology
ImageJ
Masson's trichrome
cadaver
collagen fiber
morphometric analysis

ASJC Scopus subject areas

Dermatology

Access to Document

10.1111/srt.12776

Cite this

@article{d96e203772d241e493f2f5e38dee891d,

title = "A simple morphometric analysis method for dermal microstructure using color thresholding and moments",

abstract = "Background: Proper assessment of dermal collagen fibers by dermatologists and researchers is essential. Histologic evaluation methods have limitations. We present a simple method for measurement of collagen fibers in human skin using Masson's trichrome staining. Materials and Methods: Normal skin specimens from a cadaver were processed with Masson's trichrome, which can effectively stain collagen fibers blue with aniline dye. Optical photomicrographs of these slides were analyzed using ImageJ software. Color image processing, a histogram-based function of ImageJ for image segmentation, was performed with color moments thresholding technique. We selected blue areas by adjusting the blue channel to include specific values. The selected areas were highlighted and evaluated. We divided the image into layers of 0.09-mm2 areas from the top to bottom of the dermis. Each area was cropped and evaluated. Results: Quantitative assessment yielded the quantitative size occupied by collagen fibers in an area of 0.09 mm2. Calculation of the percentage in each area can be used to determine the density of collagen fibers. Conclusion: Measurements obtained with our method can be applied to research on dermal collagen fibers. We present a convenient quantitative assessment method for the dermal constituents in Masson's trichrome-stained slides.",

keywords = "Histology, ImageJ, Masson's trichrome, cadaver, collagen fiber, morphometric analysis",

author = "Hong, {Ji Hyuck} and Kim, {Dai Hyun} and Rhyu, {Im Joo} and Kye, {Young Chul} and Ahn, {Hyo Hyun}",

note = "Funding Information: This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Science, ICT & Future Planning (NRF‐2017R1A2B4011001). Publisher Copyright: {\textcopyright} 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd",

year = "2020",

month = jan,

day = "1",

doi = "10.1111/srt.12776",

language = "English",

volume = "26",

pages = "132--136",

journal = "Skin Research and Technology",

issn = "0909-752X",

publisher = "Wiley-Blackwell Publishing Ltd",

number = "1",

}

TY - JOUR

T1 - A simple morphometric analysis method for dermal microstructure using color thresholding and moments

AU - Hong, Ji Hyuck

AU - Kim, Dai Hyun

AU - Rhyu, Im Joo

AU - Kye, Young Chul

AU - Ahn, Hyo Hyun

N1 - Funding Information: This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Science, ICT & Future Planning (NRF‐2017R1A2B4011001). Publisher Copyright: © 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd

PY - 2020/1/1

Y1 - 2020/1/1

N2 - Background: Proper assessment of dermal collagen fibers by dermatologists and researchers is essential. Histologic evaluation methods have limitations. We present a simple method for measurement of collagen fibers in human skin using Masson's trichrome staining. Materials and Methods: Normal skin specimens from a cadaver were processed with Masson's trichrome, which can effectively stain collagen fibers blue with aniline dye. Optical photomicrographs of these slides were analyzed using ImageJ software. Color image processing, a histogram-based function of ImageJ for image segmentation, was performed with color moments thresholding technique. We selected blue areas by adjusting the blue channel to include specific values. The selected areas were highlighted and evaluated. We divided the image into layers of 0.09-mm2 areas from the top to bottom of the dermis. Each area was cropped and evaluated. Results: Quantitative assessment yielded the quantitative size occupied by collagen fibers in an area of 0.09 mm2. Calculation of the percentage in each area can be used to determine the density of collagen fibers. Conclusion: Measurements obtained with our method can be applied to research on dermal collagen fibers. We present a convenient quantitative assessment method for the dermal constituents in Masson's trichrome-stained slides.

AB - Background: Proper assessment of dermal collagen fibers by dermatologists and researchers is essential. Histologic evaluation methods have limitations. We present a simple method for measurement of collagen fibers in human skin using Masson's trichrome staining. Materials and Methods: Normal skin specimens from a cadaver were processed with Masson's trichrome, which can effectively stain collagen fibers blue with aniline dye. Optical photomicrographs of these slides were analyzed using ImageJ software. Color image processing, a histogram-based function of ImageJ for image segmentation, was performed with color moments thresholding technique. We selected blue areas by adjusting the blue channel to include specific values. The selected areas were highlighted and evaluated. We divided the image into layers of 0.09-mm2 areas from the top to bottom of the dermis. Each area was cropped and evaluated. Results: Quantitative assessment yielded the quantitative size occupied by collagen fibers in an area of 0.09 mm2. Calculation of the percentage in each area can be used to determine the density of collagen fibers. Conclusion: Measurements obtained with our method can be applied to research on dermal collagen fibers. We present a convenient quantitative assessment method for the dermal constituents in Masson's trichrome-stained slides.

KW - Histology

KW - ImageJ

KW - Masson's trichrome

KW - cadaver

KW - collagen fiber

KW - morphometric analysis

UR - http://www.scopus.com/inward/record.url?scp=85074035953&partnerID=8YFLogxK

U2 - 10.1111/srt.12776

DO - 10.1111/srt.12776

M3 - Article

C2 - 31541511

AN - SCOPUS:85074035953

SN - 0909-752X

VL - 26

SP - 132

EP - 136

JO - Skin Research and Technology

JF - Skin Research and Technology

IS - 1

ER -

A simple morphometric analysis method for dermal microstructure using color thresholding and moments

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this