A simple technique to convert sitting-drop vapor diffusion into hanging-drop vapor diffusion by solidifying the reservoir solution with agarose

Tae Woong Whon, Yong Hwan Lee, Dong Shan An, Hyun Kyu Song, Song Gun Kim

    Research output: Contribution to journalArticlepeer-review

    10 Citations (Scopus)

    Abstract

    A simple protocol to convert sitting-drop vapor-diffusion plating into a hanging-drop vapor-diffusion experiment in protein crystallization is reported. After making a sitting-drop plate, agarose solution was added to solidify the reservoir solution, and the plates were incubated upside down. Crystallization experiments with hen egg white lysozyme, thaumatin and glucose isomerase showed that the upside-down sitting-drop method could produce single crystals with all the benefits of the hanging-drop crystallization method.

    Original languageEnglish
    Pages (from-to)975-976
    Number of pages2
    JournalJournal of Applied Crystallography
    Volume42
    Issue number5
    DOIs
    Publication statusPublished - 2009

    Keywords

    • Agarose gel
    • Crystallization
    • Glucose isomerase
    • Lysozyme
    • Protein crystallography
    • Solidified reservoirs
    • Thaumatin
    • Upside-down sitting-drop vapor diffusion

    ASJC Scopus subject areas

    • General Biochemistry,Genetics and Molecular Biology

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