A simple urine test by 3D-plus-3D immunoassay guides precise in vitro cancer diagnosis

Hye Hyun Kim, Ok Jeong Moon, Yong Hwan Seol, Jeewon Lee

Research output: Contribution to journalArticlepeer-review

Abstract

Although a variety of urinary cancer markers are available for in vitro diagnosis, inherent problems of urine environment—containing various inorganic/organic ions/molecules that vary in concentration over a 20-fold range or more and significantly attenuate antibody avidity for markers—render conventional immunoassays unsuitable, remaining unresolved and a major challenge. Here we developed a 3D-plus-3D (3p3) immunoassay method, based on a single-step urinary marker detection by 3D-antibody probes, which are free of steric hindrance and capable of omnidirectional capture of markers in a 3D solution. The 3p3 immunoassay showed an excellent performance in the diagnosis of prostate cancer (PCa) through detecting PCa-specific urinary engrailed-2 protein, demonstrating 100% sensitivity and 100% specificity with the urine specimens of PCa-related and other related disease patients and healthy individuals. This innovative approach holds a great potential in opening up a novel clinical route for precise in vitro cancer diagnosis and also pushing urine immunoassay closer to more widespread adoption.

Original languageEnglish
Article numbere10489
JournalBioengineering and Translational Medicine
Volume8
Issue number3
DOIs
Publication statusPublished - 2023 May

Bibliographical note

Funding Information:
This study was supported by the National Research Foundation of Korea (NRF) grant funded by Korea government (MEST) (Grant No. NRF2019R1A2C3005771). The financial support of Cellemedy Inc. is also appreciated. The biospecimens and data used for this study were provided by the Seoul National University Hospital Biomedical Research Institute, Biobank of Chungnam National University Hospital, and Biobank of Chungbuk National University Hospital.

Funding Information:
This study was supported by the National Research Foundation of Korea (NRF) grant funded by Korea government (MEST) (Grant No. NRF2019R1A2C3005771). The financial support of Cellemedy Inc. is also appreciated. The biospecimens and data used for this study were provided by the Seoul National University Hospital Biomedical Research Institute, Biobank of Chungnam National University Hospital, and Biobank of Chungbuk National University Hospital.

Funding Information:
Cellemedy Inc.; National Research Foundation of Korea, Grant/Award Number: NRF2019R1A2C3005771 Funding information

Publisher Copyright:
© 2023 The Authors. Bioengineering & Translational Medicine published by Wiley Periodicals LLC on behalf of American Institute of Chemical Engineers.

Keywords

  • 3D-IgG probes
  • 3D-plus-3D immunoassay
  • in vitro cancer diagnosis
  • signal self-enhancement
  • urine test

ASJC Scopus subject areas

  • Biotechnology
  • Biomedical Engineering
  • Pharmaceutical Science

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