Abstract
The design of the first dual-purpose activity-based probe of monoamine oxidase B (MAO-B) is reported. This probe is highly selective towards MAO-B, even at high MAO-A expression levels, and could sensitively report endogenous MAO-B activities by both in situ proteome profiling and live-cell bioimaging. With a built-in imaging module as part of the probe design, the probe was able to accomplish what all previously reported MAO-B imaging probes failed to do thus far: the live-cell imaging of MAO-B activities without encountering diffusion problems.
Original language | English |
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Pages (from-to) | 10821-10825 |
Number of pages | 5 |
Journal | Angewandte Chemie - International Edition |
Volume | 54 |
Issue number | 37 |
DOIs | |
Publication status | Published - 2015 Sept 1 |
Externally published | Yes |
Bibliographical note
Publisher Copyright:© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Keywords
- Parkinson's disease
- activity-based probes
- imaging
- monoamine oxidases
- proteomics
ASJC Scopus subject areas
- Catalysis
- General Chemistry