A Small-Molecule Probe for Selective Profiling and Imaging of Monoamine Oxidase B Activities in Models of Parkinson's Disease

Lin Li; Cheng Wu Zhang; Jingyan Ge; Linghui Qian; Bing Han Chai; Qing Zhu; Jun Seok Lee; Kah Leong Lim; Shao Q. Yao

doi:10.1002/anie.201504441

A Small-Molecule Probe for Selective Profiling and Imaging of Monoamine Oxidase B Activities in Models of Parkinson's Disease

Lin Li
, Cheng Wu Zhang
, Jingyan Ge
, Linghui Qian
, Bing Han Chai
, Qing Zhu
, Jun Seok Lee
, Kah Leong Lim
, Shao Q. Yao^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

95 Citations (Scopus)

Abstract

The design of the first dual-purpose activity-based probe of monoamine oxidase B (MAO-B) is reported. This probe is highly selective towards MAO-B, even at high MAO-A expression levels, and could sensitively report endogenous MAO-B activities by both in situ proteome profiling and live-cell bioimaging. With a built-in imaging module as part of the probe design, the probe was able to accomplish what all previously reported MAO-B imaging probes failed to do thus far: the live-cell imaging of MAO-B activities without encountering diffusion problems.

Original language	English
Pages (from-to)	10821-10825
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	54
Issue number	37
DOIs	https://doi.org/10.1002/anie.201504441
Publication status	Published - 2015 Sept 1
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Keywords

Parkinson's disease
activity-based probes
imaging
monoamine oxidases
proteomics

ASJC Scopus subject areas

Catalysis
General Chemistry

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10.1002/anie.201504441

Cite this

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title = "A Small-Molecule Probe for Selective Profiling and Imaging of Monoamine Oxidase B Activities in Models of Parkinson's Disease",

abstract = "The design of the first dual-purpose activity-based probe of monoamine oxidase B (MAO-B) is reported. This probe is highly selective towards MAO-B, even at high MAO-A expression levels, and could sensitively report endogenous MAO-B activities by both in situ proteome profiling and live-cell bioimaging. With a built-in imaging module as part of the probe design, the probe was able to accomplish what all previously reported MAO-B imaging probes failed to do thus far: the live-cell imaging of MAO-B activities without encountering diffusion problems.",

keywords = "Parkinson's disease, activity-based probes, imaging, monoamine oxidases, proteomics",

author = "Lin Li and Zhang, \{Cheng Wu\} and Jingyan Ge and Linghui Qian and Chai, \{Bing Han\} and Qing Zhu and Lee, \{Jun Seok\} and Lim, \{Kah Leong\} and Yao, \{Shao Q.\}",

note = "Publisher Copyright: {\textcopyright} 2015 WILEY-VCH Verlag GmbH \& Co. KGaA, Weinheim.",

year = "2015",

month = sep,

day = "1",

doi = "10.1002/anie.201504441",

language = "English",

volume = "54",

pages = "10821--10825",

journal = "Angewandte Chemie - International Edition",

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T1 - A Small-Molecule Probe for Selective Profiling and Imaging of Monoamine Oxidase B Activities in Models of Parkinson's Disease

AU - Li, Lin

AU - Zhang, Cheng Wu

AU - Ge, Jingyan

AU - Qian, Linghui

AU - Chai, Bing Han

AU - Zhu, Qing

AU - Lee, Jun Seok

AU - Lim, Kah Leong

AU - Yao, Shao Q.

PY - 2015/9/1

Y1 - 2015/9/1

N2 - The design of the first dual-purpose activity-based probe of monoamine oxidase B (MAO-B) is reported. This probe is highly selective towards MAO-B, even at high MAO-A expression levels, and could sensitively report endogenous MAO-B activities by both in situ proteome profiling and live-cell bioimaging. With a built-in imaging module as part of the probe design, the probe was able to accomplish what all previously reported MAO-B imaging probes failed to do thus far: the live-cell imaging of MAO-B activities without encountering diffusion problems.

AB - The design of the first dual-purpose activity-based probe of monoamine oxidase B (MAO-B) is reported. This probe is highly selective towards MAO-B, even at high MAO-A expression levels, and could sensitively report endogenous MAO-B activities by both in situ proteome profiling and live-cell bioimaging. With a built-in imaging module as part of the probe design, the probe was able to accomplish what all previously reported MAO-B imaging probes failed to do thus far: the live-cell imaging of MAO-B activities without encountering diffusion problems.

KW - Parkinson's disease

KW - activity-based probes

KW - imaging

KW - monoamine oxidases

KW - proteomics

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DO - 10.1002/anie.201504441

M3 - Article

C2 - 26205378

AN - SCOPUS:84940720973

SN - 1433-7851

VL - 54

SP - 10821

EP - 10825

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 37

ER -

A Small-Molecule Probe for Selective Profiling and Imaging of Monoamine Oxidase B Activities in Models of Parkinson's Disease

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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