A small-molecule two-photon probe for nitric oxide in living tissues

Eun Won Seo; Ji Hee Han; Cheol Ho Heo; Jae Ho Shin; Hwan Myung Kim; Bong Rae Cho

doi:10.1002/chem.201201197

A small-molecule two-photon probe for nitric oxide in living tissues

Eun Won Seo, Ji Hee Han, Cheol Ho Heo, Jae Ho Shin, Hwan Myung Kim, Bong Rae Cho

Research output: Contribution to journal › Article › peer-review

55 Citations (Scopus)

Abstract

Two-photon microscopy (TPM) has become an indispensable tool in the study of biology and medicine due to the capability of this method for molecular imaging deep inside intact tissues. For the maximum utilization of TPM, a variety of two-photon (TP) probes for specific applications are needed. In this article, we report a small-molecule TP probe (ANO1) for nitric oxide (NO) that shows a rapid and specific NO response, a 68-fold fluorescence enhancement in response to NO, and a maximum TP-action cross-section of 170-GM (GM: 10 ^-50-cm⁴ photon^-1) upon reaction with excess NO. This probe can be easily loaded into cells and tissues and can real-time monitor NO in living tissues at 100-180-μm depth for longer than 1200-s through the use of TPM, with minimum interference from other biologically relevant species.

Original language	English
Pages (from-to)	12388-12394
Number of pages	7
Journal	Chemistry - A European Journal
Volume	18
Issue number	39
DOIs	https://doi.org/10.1002/chem.201201197
Publication status	Published - 2012 Sept 24

Keywords

fluorescent probes
living tissues
nitrogen oxides
two-photon microscopy
two-photon probe

ASJC Scopus subject areas

Catalysis
Organic Chemistry

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10.1002/chem.201201197

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title = "A small-molecule two-photon probe for nitric oxide in living tissues",

abstract = "Two-photon microscopy (TPM) has become an indispensable tool in the study of biology and medicine due to the capability of this method for molecular imaging deep inside intact tissues. For the maximum utilization of TPM, a variety of two-photon (TP) probes for specific applications are needed. In this article, we report a small-molecule TP probe (ANO1) for nitric oxide (NO) that shows a rapid and specific NO response, a 68-fold fluorescence enhancement in response to NO, and a maximum TP-action cross-section of 170-GM (GM: 10 -50-cm4 photon-1) upon reaction with excess NO. This probe can be easily loaded into cells and tissues and can real-time monitor NO in living tissues at 100-180-μm depth for longer than 1200-s through the use of TPM, with minimum interference from other biologically relevant species.",

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AU - Han, Ji Hee

AU - Heo, Cheol Ho

AU - Shin, Jae Ho

AU - Kim, Hwan Myung

AU - Cho, Bong Rae

PY - 2012/9/24

Y1 - 2012/9/24

N2 - Two-photon microscopy (TPM) has become an indispensable tool in the study of biology and medicine due to the capability of this method for molecular imaging deep inside intact tissues. For the maximum utilization of TPM, a variety of two-photon (TP) probes for specific applications are needed. In this article, we report a small-molecule TP probe (ANO1) for nitric oxide (NO) that shows a rapid and specific NO response, a 68-fold fluorescence enhancement in response to NO, and a maximum TP-action cross-section of 170-GM (GM: 10 -50-cm4 photon-1) upon reaction with excess NO. This probe can be easily loaded into cells and tissues and can real-time monitor NO in living tissues at 100-180-μm depth for longer than 1200-s through the use of TPM, with minimum interference from other biologically relevant species.

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A small-molecule two-photon probe for nitric oxide in living tissues

Abstract

Keywords

ASJC Scopus subject areas

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