A small-molecule two-photon probe for nitric oxide in living tissues

Eun Won Seo, Ji Hee Han, Cheol Ho Heo, Jae Ho Shin, Hwan Myung Kim, Bong Rae Cho

Research output: Contribution to journalArticlepeer-review

53 Citations (Scopus)


Two-photon microscopy (TPM) has become an indispensable tool in the study of biology and medicine due to the capability of this method for molecular imaging deep inside intact tissues. For the maximum utilization of TPM, a variety of two-photon (TP) probes for specific applications are needed. In this article, we report a small-molecule TP probe (ANO1) for nitric oxide (NO) that shows a rapid and specific NO response, a 68-fold fluorescence enhancement in response to NO, and a maximum TP-action cross-section of 170-GM (GM: 10 -50-cm4 photon-1) upon reaction with excess NO. This probe can be easily loaded into cells and tissues and can real-time monitor NO in living tissues at 100-180-μm depth for longer than 1200-s through the use of TPM, with minimum interference from other biologically relevant species.

Original languageEnglish
Pages (from-to)12388-12394
Number of pages7
JournalChemistry - A European Journal
Issue number39
Publication statusPublished - 2012 Sept 24


  • fluorescent probes
  • living tissues
  • nitrogen oxides
  • two-photon microscopy
  • two-photon probe

ASJC Scopus subject areas

  • Catalysis
  • Organic Chemistry


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