A sweetpotato SRD1 promoter confers strong root-, taproot-, and tuber-specific expression in Arabidopsis, carrot, and potato

Seol Ah Noh, Haeng Soon Lee, Gyung Hye Huh, Mi Joung Oh, Kyung Hee Paek, Jeong Sheop Shin, Jung Myung Bae

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)


Harvestable, starch-storing organs of plants, such as fleshy taproots and tubers, are important agronomic products that are also suitable target organs for use in the molecular farming of recombinant proteins due to their strong sink strength. To exploit a promoter directing strong expression restricted to these storage organs, we isolated the promoter region (3.0 kb) of SRD1 from sweetpotato (Ipomoea batatas cv. 'White Star') and characterized its activity in transgenic Arabidopsis, carrot, and potato using the β-glucuronidase (GUS) gene (uidA) as a reporter gene. The SRD1 promoter conferred root-specific expression in transgenic Arabidopsis, with SRD1 promoter activity increasing in response to exogenous IAA. A time-course study of the effect of IAA (50 μM) revealed a maximum increase in SRD1 promoter activity at 24 h post-treatment initiation. A serial 5′ deletion analysis of the SRD1 promoter identified regions related to IAA-inducible expression as well as regions containing positive and negative elements, respectively, controlling the expression level. In transgenic carrot, the SRD1 promoter mediated strong taproot-specific expression, as evidenced by GUS staining being strong in almost the entire taproot, including secondary phloem, secondary xylem and vascular cambium. The activity of the SRD1 promoter gradually increased with increasing diameter of the taproot in the transgenic carrot and was 10.71-fold higher than that of the CaMV35S promoter. The SRD1 promoter also directed strong tuber-specific expression in transgenic potato. Taken together, these results demonstrate that the SRD1 promoter directs strong expression restricted to the underground storage organs, such as fleshy taproots and tubers, as well as fibrous root tissues.

Original languageEnglish
Pages (from-to)265-278
Number of pages14
JournalTransgenic Research
Issue number2
Publication statusPublished - 2012 Apr

Bibliographical note

Funding Information:
Acknowledgments This work was supported by grants from the Next-Generation BioGreen 21 Program (No. PJ008076032011 and No. PJ008103022011), Rural Development Administration, Republic of Korea, and a grant (No. 2010-0002903) from the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology.


  • Carrot
  • Potato
  • Promoter
  • Root
  • Sweetpotato
  • Taproot
  • Tuber

ASJC Scopus subject areas

  • Genetics
  • Animal Science and Zoology
  • Agronomy and Crop Science
  • Biotechnology


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