A three-dimensional nanostructured array of protein nanoparticles

Hyuk Seong Seo, Seong Eun Kim, Jin Seung Park, Jong Hwan Lee, Ki Yeon Yang, Heon Lee, Kyung Eun Lee, Sung Sik Han, Jeewon Lee

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)


Here a novel technique is reported to construct a three-dimensional (3D) array of well-defined and controllable multilayered nanostructures of proteins that is based on alternate layer-by-layer assembly of bacterial protein nanoparticles and DNA on a patterned array of gold dots. This is the first report on protein-based multilayer stacking, which has the following significant advantages over conventional multilayer assemblies: 1) avoiding hazardous chemicals, the multilayer assembly is implemented in aqueous solution under mild temperature and pH conditions over a relatively short period; 2) direct multilayer growth from designated position is possible by controlling the aspect ratio; 3) multicomponent stacking can be easily performed through alternate stacking of different building blocks (in this case protein nanoparticles); and 4) a wide variety of 3D arrays can be constructed using various functionalized protein nanoparticles that are easily prepared through a simple genetic engineering approach. In this study, as a proof of concept, the developed 3D and patterned arrays of protein nanoparticle multilayers are successfully applied to the multiplexed bioassays of breast and colorectal cancer markers.

Original languageEnglish
Pages (from-to)4055-4061
Number of pages7
JournalAdvanced Functional Materials
Issue number23
Publication statusPublished - 2010 Dec 8


  • biosensors
  • nanoparticles
  • nanostructures
  • protein engineering

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • General Chemistry
  • General Materials Science
  • Electrochemistry
  • Biomaterials


Dive into the research topics of 'A three-dimensional nanostructured array of protein nanoparticles'. Together they form a unique fingerprint.

Cite this