Abstract
A two-stage minibioreactor system was successfully developed for continuous toxicity monitoring. This system consists of two minibioreactors in series. Recombinant Escherichia coli DPD2794 containing a RecA::luxCDABE fusion as a model strain was utilized to monitor environmental insults to DNA, with mitomycin C as a model toxicant. Pulse type exposures were used to evaluate the system's reproducibility and reliability. Step inputs of mitomycin C have been adopted to show the system's stability. The system's ability to monitor the possible upsets or accidental discharges of toxic chemicals was also evaluated with these step insults. All the data demonstrated that this two-stage minibioreactor system using recombinant bacteria containing stress promoters fused with lux genes is quite appropriate for continuous toxicity monitoring. Long-term operation and minimized media-usage have been investigated. Thus application to many different areas, including an early warning system of wastewater biotreatment plant upsets and the monitoring and tracking of accidental spills, discharges or failures in plant operation are plausible.
| Original language | English |
|---|---|
| Pages (from-to) | 355-361 |
| Number of pages | 7 |
| Journal | Biosensors and Bioelectronics |
| Volume | 14 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 1999 Apr 30 |
| Externally published | Yes |
Bibliographical note
Funding Information:Authors wish to thank Dr LaRossa for the strain used in this study and very useful discussion and comments in preparation of this paper. This work was supported by Korean Science and Engineering Foundation (Grant # G70580) and authors are grateful for the support.
Keywords
- Bioluminescence
- Genotoxicity
- Recombinant Escherichia coli
- Stress promotors
- Two-stage minibioreactors
ASJC Scopus subject areas
- Biotechnology
- Biophysics
- Biomedical Engineering
- Electrochemistry
