A versatile assay for monitoring in vivo-like transendothelial migration of neutrophils.

Sewoon Han; Ji Jing Yan; Yoojin Shin; Jessie J. Jeon; Jihee Won; Hyo Eun Jeong; Roger D. Kamm; Young Joon Kim; Seok Chung

doi:10.1039/c2lc40445a

A versatile assay for monitoring in vivo-like transendothelial migration of neutrophils.

Sewoon Han, Ji Jing Yan, Yoojin Shin, Jessie J. Jeon, Jihee Won, Hyo Eun Jeong, Roger D. Kamm, Young Joon Kim, Seok Chung

School of Mechanical Engineering

Research output: Contribution to journal › Article › peer-review

75 Citations (Scopus)

Abstract

Spatiotemporal analysis of the inflammatory response has been limited by the difficulties of in vivo imaging and reconstitution of inflammation in vitro. Here, we present a novel method for establishing in vivo-like inflammatory models in a microfluidic device and quantitatively measuring the three-dimensional transmigration of neutrophils during the inflammatory process. This enabled us to concurrently characterize transendothelial migration behaviors of neutrophils under the influence of various inflammatory stimuli.

Original language	English
Pages (from-to)	3861-3865
Number of pages	5
Journal	Lab on a Chip
Volume	12
Issue number	20
DOIs	https://doi.org/10.1039/c2lc40445a
Publication status	Published - 2012 Oct 21

ASJC Scopus subject areas

Bioengineering
Biochemistry
Chemistry(all)
Biomedical Engineering

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10.1039/c2lc40445a

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abstract = "Spatiotemporal analysis of the inflammatory response has been limited by the difficulties of in vivo imaging and reconstitution of inflammation in vitro. Here, we present a novel method for establishing in vivo-like inflammatory models in a microfluidic device and quantitatively measuring the three-dimensional transmigration of neutrophils during the inflammatory process. This enabled us to concurrently characterize transendothelial migration behaviors of neutrophils under the influence of various inflammatory stimuli.",

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AU - Won, Jihee

AU - Jeong, Hyo Eun

AU - Kamm, Roger D.

AU - Kim, Young Joon

AU - Chung, Seok

PY - 2012/10/21

Y1 - 2012/10/21

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AB - Spatiotemporal analysis of the inflammatory response has been limited by the difficulties of in vivo imaging and reconstitution of inflammation in vitro. Here, we present a novel method for establishing in vivo-like inflammatory models in a microfluidic device and quantitatively measuring the three-dimensional transmigration of neutrophils during the inflammatory process. This enabled us to concurrently characterize transendothelial migration behaviors of neutrophils under the influence of various inflammatory stimuli.

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A versatile assay for monitoring in vivo-like transendothelial migration of neutrophils.

Abstract

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