A whole cell bioluminescent biosensor for the detection of membrane-damaging toxicity

Sue Hyung Choi; Man Bock Gu

doi:10.1007/BF02931916

A whole cell bioluminescent biosensor for the detection of membrane-damaging toxicity

Sue Hyung Choi, Man Bock Gu

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

The recombinant bacteria strain DPD2540, containing a fabA::luxCDABE fusion, was used to detect the toxicity of various chemicals in this study. Membrane damaging agents such as phenol, ethanol, and cerulenin induced a rapid bioluminescent response from this strain. Other toxic agents, such as DNA-damaging or oxidative-damaging chemicals, showed a delayed bioluminescent response in which the maximum peak appeared over 150mim after induction. This strain was also tested for measurement of toxicity in field samples such as wastewater and river water effluents.

Original language	English
Pages (from-to)	59-62
Number of pages	4
Journal	Biotechnology and Bioprocess Engineering
Volume	4
Issue number	1
DOIs	https://doi.org/10.1007/BF02931916
Publication status	Published - 1999
Externally published	Yes

Keywords

Bioluminescent bacteria
Bioluminescent response
Membrane damage
fabA promoter
lux gene

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology
Biomedical Engineering

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10.1007/BF02931916

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title = "A whole cell bioluminescent biosensor for the detection of membrane-damaging toxicity",

abstract = "The recombinant bacteria strain DPD2540, containing a fabA::luxCDABE fusion, was used to detect the toxicity of various chemicals in this study. Membrane damaging agents such as phenol, ethanol, and cerulenin induced a rapid bioluminescent response from this strain. Other toxic agents, such as DNA-damaging or oxidative-damaging chemicals, showed a delayed bioluminescent response in which the maximum peak appeared over 150mim after induction. This strain was also tested for measurement of toxicity in field samples such as wastewater and river water effluents.",

keywords = "Bioluminescent bacteria, Bioluminescent response, Membrane damage, fabA promoter, lux gene",

author = "Choi, {Sue Hyung} and Gu, {Man Bock}",

note = "Funding Information: Acknowledgements Authors wish to thank Dr. LaRossa in DuPont Co. in the U.S.A. and Shimshon Belkin in Hebrew University of Jerusalem in Israel for the strain used in this study and very useful discussions in preparation of this paper. This work was supported by Korea Research Foundation (Grant # 97-F-20) and authors are grateful for the support.",

year = "1999",

doi = "10.1007/BF02931916",

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T1 - A whole cell bioluminescent biosensor for the detection of membrane-damaging toxicity

AU - Choi, Sue Hyung

AU - Gu, Man Bock

N1 - Funding Information: Acknowledgements Authors wish to thank Dr. LaRossa in DuPont Co. in the U.S.A. and Shimshon Belkin in Hebrew University of Jerusalem in Israel for the strain used in this study and very useful discussions in preparation of this paper. This work was supported by Korea Research Foundation (Grant # 97-F-20) and authors are grateful for the support.

PY - 1999

Y1 - 1999

N2 - The recombinant bacteria strain DPD2540, containing a fabA::luxCDABE fusion, was used to detect the toxicity of various chemicals in this study. Membrane damaging agents such as phenol, ethanol, and cerulenin induced a rapid bioluminescent response from this strain. Other toxic agents, such as DNA-damaging or oxidative-damaging chemicals, showed a delayed bioluminescent response in which the maximum peak appeared over 150mim after induction. This strain was also tested for measurement of toxicity in field samples such as wastewater and river water effluents.

AB - The recombinant bacteria strain DPD2540, containing a fabA::luxCDABE fusion, was used to detect the toxicity of various chemicals in this study. Membrane damaging agents such as phenol, ethanol, and cerulenin induced a rapid bioluminescent response from this strain. Other toxic agents, such as DNA-damaging or oxidative-damaging chemicals, showed a delayed bioluminescent response in which the maximum peak appeared over 150mim after induction. This strain was also tested for measurement of toxicity in field samples such as wastewater and river water effluents.

KW - Bioluminescent bacteria

KW - Bioluminescent response

KW - Membrane damage

KW - fabA promoter

KW - lux gene

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A whole cell bioluminescent biosensor for the detection of membrane-damaging toxicity

Abstract

Keywords

ASJC Scopus subject areas

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