Abscisic acid does not disrupt either the arabidopsis FCA-FY interaction or its rice counterpart in vitro

Yun Hee Jang; Jeong Hwan Lee; Jeong Kook Kim

doi:10.1093/pcp/pcn151

Abscisic acid does not disrupt either the arabidopsis FCA-FY interaction or its rice counterpart in vitro

Yun Hee Jang, Jeong Hwan Lee, Jeong Kook Kim

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

We examined the effect of (+)-ABA on the in vitro interaction of rice FCA and FY homologs, OsFCA and OsFY. From this analysis, we found no disruption of the OsFCA-OsFY complexes by ABA treatment. This result prompted us to examine the effect of ABA on the FCA-FY interaction. In these experiments, we could not reproduce the inhibitory effect of (+)-ABA on the interaction between FCA and FY. Based on these combined results, we believe that the inhibitory effect of (+)-ABA on the FCA-FY interaction should be cautiously reconsidered.

Original language	English
Pages (from-to)	1898-1901
Number of pages	4
Journal	Plant and Cell Physiology
Volume	49
Issue number	12
DOIs	https://doi.org/10.1093/pcp/pcn151
Publication status	Published - 2008 Dec

Bibliographical note

Funding Information:
The Plant Signaling Network Research Center, Korea Science and Engineering Foundation Republic of Korea; the Korean Ministry of Education and Human Resources Development BK21 research fellowship (to Y.H.J); the Korea Research Foundation (grant No. KRF-2007-359-C00023 to J.H.L) funded by the Korea Government (MOEHRD).

Keywords

ABA
ABA receptor
FCA and FY interaction
GST pull-down assay
OsFCA and OsFY interaction

ASJC Scopus subject areas

Physiology
Plant Science
Cell Biology

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10.1093/pcp/pcn151

Cite this

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title = "Abscisic acid does not disrupt either the arabidopsis FCA-FY interaction or its rice counterpart in vitro",

abstract = "We examined the effect of (+)-ABA on the in vitro interaction of rice FCA and FY homologs, OsFCA and OsFY. From this analysis, we found no disruption of the OsFCA-OsFY complexes by ABA treatment. This result prompted us to examine the effect of ABA on the FCA-FY interaction. In these experiments, we could not reproduce the inhibitory effect of (+)-ABA on the interaction between FCA and FY. Based on these combined results, we believe that the inhibitory effect of (+)-ABA on the FCA-FY interaction should be cautiously reconsidered.",

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note = "Funding Information: The Plant Signaling Network Research Center, Korea Science and Engineering Foundation Republic of Korea; the Korean Ministry of Education and Human Resources Development BK21 research fellowship (to Y.H.J); the Korea Research Foundation (grant No. KRF-2007-359-C00023 to J.H.L) funded by the Korea Government (MOEHRD).",

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AU - Kim, Jeong Kook

N1 - Funding Information: The Plant Signaling Network Research Center, Korea Science and Engineering Foundation Republic of Korea; the Korean Ministry of Education and Human Resources Development BK21 research fellowship (to Y.H.J); the Korea Research Foundation (grant No. KRF-2007-359-C00023 to J.H.L) funded by the Korea Government (MOEHRD).

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N2 - We examined the effect of (+)-ABA on the in vitro interaction of rice FCA and FY homologs, OsFCA and OsFY. From this analysis, we found no disruption of the OsFCA-OsFY complexes by ABA treatment. This result prompted us to examine the effect of ABA on the FCA-FY interaction. In these experiments, we could not reproduce the inhibitory effect of (+)-ABA on the interaction between FCA and FY. Based on these combined results, we believe that the inhibitory effect of (+)-ABA on the FCA-FY interaction should be cautiously reconsidered.

AB - We examined the effect of (+)-ABA on the in vitro interaction of rice FCA and FY homologs, OsFCA and OsFY. From this analysis, we found no disruption of the OsFCA-OsFY complexes by ABA treatment. This result prompted us to examine the effect of ABA on the FCA-FY interaction. In these experiments, we could not reproduce the inhibitory effect of (+)-ABA on the interaction between FCA and FY. Based on these combined results, we believe that the inhibitory effect of (+)-ABA on the FCA-FY interaction should be cautiously reconsidered.

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KW - ABA receptor

KW - FCA and FY interaction

KW - GST pull-down assay

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JO - Plant and Cell Physiology

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ER -

Abscisic acid does not disrupt either the arabidopsis FCA-FY interaction or its rice counterpart in vitro

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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