Accelerated osteogenic differentiation of MC3T3-E1 cells by lactoferrin-conjugated nanodiamonds through enhanced anti-oxidant and anti- inflammatory effects

Sung Eun Kim; Somang Choi; Jae Young Hong; Kyu Sik Shim; Tae Hoon Kim; Kyeongsoon Park; Suk Ha Lee

doi:10.3390/nano10010050

Accelerated osteogenic differentiation of MC3T3-E1 cells by lactoferrin-conjugated nanodiamonds through enhanced anti-oxidant and anti- inflammatory effects

Sung Eun Kim, Somang Choi, Jae Young Hong, Kyu Sik Shim, Tae Hoon Kim, Kyeongsoon Park, Suk Ha Lee

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

The purpose of this study was to investigate the effects of lactoferrin (LF)-conjugated nanodiamonds (NDs) in vitro on both anti-oxidant and anti-inflammation activity as well as osteogenic promotion. The application of LF-NDs resulted in sustained release of LF for up to 7 days. In vitro anti-oxidant analyses performed using Dichlorofluorescin diacetate (DCF-DA) assay and cell proliferation studies showed that LF (50 μg)-NDs effectively scavenged the reactive oxygen species (ROS) in MC3T3-E1 cells (osteoblast-like cells) after H2O2 treatment and increased proliferation of cells after H2O2 treatment. Treatment of lipopolysaccharide (LPS)-induced MC3T3- E1 cells with LF-NDs suppressed levels of pro-inflammatory cytokines, including interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α). In addition, LF-NDs were associated with outstanding enhancement of osteogenic activity of MC3T3-E1 cells due to increased alkaline phosphatase (ALP) and calcium deposition. Our findings suggest that LF-NDs are an important substrate for alleviating ROS effects and inflammation, as well as promoting osteogenic differentiation of cells.

Original language	English
Article number	50
Journal	Nanomaterials
Volume	10
Issue number	1
DOIs	https://doi.org/10.3390/nano10010050
Publication status	Published - 2020 Jan

Bibliographical note

Funding Information:
This study was supported by grants from the Bio & Medical Technology Development Program of the NRF funded by the Korean government, MSIP (NRF-2017M3A9B3063640), and a National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIT) (No. NRF-2017R1C1B5015063).

Publisher Copyright:
© 2019 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

Anti-inflammatory
Anti-oxidant
Lactoferrin
Nanodiamonds
Osteogenic differentiation

ASJC Scopus subject areas

General Chemical Engineering
General Materials Science

Access to Document

10.3390/nano10010050

Cite this

@article{a92bb98ca7194c559463ad317b0c6151,

title = "Accelerated osteogenic differentiation of MC3T3-E1 cells by lactoferrin-conjugated nanodiamonds through enhanced anti-oxidant and anti- inflammatory effects",

abstract = "The purpose of this study was to investigate the effects of lactoferrin (LF)-conjugated nanodiamonds (NDs) in vitro on both anti-oxidant and anti-inflammation activity as well as osteogenic promotion. The application of LF-NDs resulted in sustained release of LF for up to 7 days. In vitro anti-oxidant analyses performed using Dichlorofluorescin diacetate (DCF-DA) assay and cell proliferation studies showed that LF (50 μg)-NDs effectively scavenged the reactive oxygen species (ROS) in MC3T3-E1 cells (osteoblast-like cells) after H2O2 treatment and increased proliferation of cells after H2O2 treatment. Treatment of lipopolysaccharide (LPS)-induced MC3T3- E1 cells with LF-NDs suppressed levels of pro-inflammatory cytokines, including interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α). In addition, LF-NDs were associated with outstanding enhancement of osteogenic activity of MC3T3-E1 cells due to increased alkaline phosphatase (ALP) and calcium deposition. Our findings suggest that LF-NDs are an important substrate for alleviating ROS effects and inflammation, as well as promoting osteogenic differentiation of cells.",

keywords = "Anti-inflammatory, Anti-oxidant, Lactoferrin, Nanodiamonds, Osteogenic differentiation",

author = "Kim, {Sung Eun} and Somang Choi and Hong, {Jae Young} and Shim, {Kyu Sik} and Kim, {Tae Hoon} and Kyeongsoon Park and Lee, {Suk Ha}",

note = "Funding Information: This study was supported by grants from the Bio & Medical Technology Development Program of the NRF funded by the Korean government, MSIP (NRF-2017M3A9B3063640), and a National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIT) (No. NRF-2017R1C1B5015063). Publisher Copyright: {\textcopyright} 2019 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2020",

month = jan,

doi = "10.3390/nano10010050",

language = "English",

volume = "10",

journal = "Nanomaterials",

issn = "2079-4991",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "1",

}

TY - JOUR

T1 - Accelerated osteogenic differentiation of MC3T3-E1 cells by lactoferrin-conjugated nanodiamonds through enhanced anti-oxidant and anti- inflammatory effects

AU - Kim, Sung Eun

AU - Choi, Somang

AU - Hong, Jae Young

AU - Shim, Kyu Sik

AU - Kim, Tae Hoon

AU - Park, Kyeongsoon

AU - Lee, Suk Ha

N1 - Funding Information: This study was supported by grants from the Bio & Medical Technology Development Program of the NRF funded by the Korean government, MSIP (NRF-2017M3A9B3063640), and a National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIT) (No. NRF-2017R1C1B5015063). Publisher Copyright: © 2019 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2020/1

Y1 - 2020/1

N2 - The purpose of this study was to investigate the effects of lactoferrin (LF)-conjugated nanodiamonds (NDs) in vitro on both anti-oxidant and anti-inflammation activity as well as osteogenic promotion. The application of LF-NDs resulted in sustained release of LF for up to 7 days. In vitro anti-oxidant analyses performed using Dichlorofluorescin diacetate (DCF-DA) assay and cell proliferation studies showed that LF (50 μg)-NDs effectively scavenged the reactive oxygen species (ROS) in MC3T3-E1 cells (osteoblast-like cells) after H2O2 treatment and increased proliferation of cells after H2O2 treatment. Treatment of lipopolysaccharide (LPS)-induced MC3T3- E1 cells with LF-NDs suppressed levels of pro-inflammatory cytokines, including interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α). In addition, LF-NDs were associated with outstanding enhancement of osteogenic activity of MC3T3-E1 cells due to increased alkaline phosphatase (ALP) and calcium deposition. Our findings suggest that LF-NDs are an important substrate for alleviating ROS effects and inflammation, as well as promoting osteogenic differentiation of cells.

AB - The purpose of this study was to investigate the effects of lactoferrin (LF)-conjugated nanodiamonds (NDs) in vitro on both anti-oxidant and anti-inflammation activity as well as osteogenic promotion. The application of LF-NDs resulted in sustained release of LF for up to 7 days. In vitro anti-oxidant analyses performed using Dichlorofluorescin diacetate (DCF-DA) assay and cell proliferation studies showed that LF (50 μg)-NDs effectively scavenged the reactive oxygen species (ROS) in MC3T3-E1 cells (osteoblast-like cells) after H2O2 treatment and increased proliferation of cells after H2O2 treatment. Treatment of lipopolysaccharide (LPS)-induced MC3T3- E1 cells with LF-NDs suppressed levels of pro-inflammatory cytokines, including interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α). In addition, LF-NDs were associated with outstanding enhancement of osteogenic activity of MC3T3-E1 cells due to increased alkaline phosphatase (ALP) and calcium deposition. Our findings suggest that LF-NDs are an important substrate for alleviating ROS effects and inflammation, as well as promoting osteogenic differentiation of cells.

KW - Anti-inflammatory

KW - Anti-oxidant

KW - Lactoferrin

KW - Nanodiamonds

KW - Osteogenic differentiation

UR - http://www.scopus.com/inward/record.url?scp=85077294144&partnerID=8YFLogxK

U2 - 10.3390/nano10010050

DO - 10.3390/nano10010050

M3 - Article

AN - SCOPUS:85077294144

SN - 2079-4991

VL - 10

JO - Nanomaterials

JF - Nanomaterials

IS - 1

M1 - 50

ER -

Accelerated osteogenic differentiation of MC3T3-E1 cells by lactoferrin-conjugated nanodiamonds through enhanced anti-oxidant and anti- inflammatory effects

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this