The acetate-assisted cultivation of Eubacterium limosum KIST612 was found to provide a way for enhancing cell mass, the carbon monoxide (CO) consumption rate, and butyrate production using CO as an electron and energy source. Cell growth (146%), μmax (121%), and CO consumption rates (151%) increased significantly upon the addition of 30 mM acetate to microbial cultures. The main product of CO fermentation by E. limosum KIST612 shifted from acetate to butyrate in the presence of acetate, and 5.72 mM butyrate was produced at the end of the reaction. The resting cell experimental conditions indicated acetate uptake and an increase in the butyrate concentration. Three routes to acetate assimilation and energy conservation were suggested based on given experimental results and previously genome sequencing data. Acetate assimilation via propionate CoA-transferase (PCT) was expected to produce 1.5 mol ATP/mol butyrate, and was thus anticipated to be the most preferred route.
Bibliographical noteFunding Information:
This research was supported by grant of Center for C1 Gas Refinery Program through the National Research Foundation of Republic of Korea (NRF-2015M3D3A1A01064883 ).
- Assimilation of acetate
- Carbon monoxide fermentation
- Eubacterium limosum KIST612
- Product shift
ASJC Scopus subject areas
- Environmental Engineering
- Renewable Energy, Sustainability and the Environment
- Waste Management and Disposal