Acidic cultivation of Haematococcus pluvialis for improved astaxanthin production in the presence of a lethal fungus

Sung Won Hwang; Hong Il Choi; Sang Jun Sim

doi:10.1016/j.biortech.2019.01.080

Acidic cultivation of Haematococcus pluvialis for improved astaxanthin production in the presence of a lethal fungus

Sung Won Hwang, Hong Il Choi, Sang Jun Sim

Research output: Contribution to journal › Article › peer-review

68 Citations (Scopus)

Abstract

An acidic cultivation strategy was developed to prevent contamination of a lethal fungus Paraphysoderma sedebokerensis in Haematococcus pluvialis culture for astaxanthin production. Instead of generally used neutral pH, an acidic condition (pH 4) was applied to the cultivation, resulting in a significant inhibition of the fungal contamination. This could be ascribed to the acidity-associated denaturation of a surface protein of P. sedebokerensis, which plays an important role in recognition of H. pluvialis. Stress relief strategies including stepwise light irradiation and naturally occurring nitrogen deficiency were employed in the induction stage to minimize the reduction of astaxanthin production caused by acidic pH. Accordingly, an astaxanthin titer of 84.8 mg L⁻¹ was obtained, which is 141-fold of that from the completely contaminated culture and double of that without the stress relief methods. This strategy provides a persistent contamination control method that can be used for practical astaxanthin production by H. pluvialis.

Original language	English
Pages (from-to)	138-144
Number of pages	7
Journal	Bioresource technology
Volume	278
DOIs	https://doi.org/10.1016/j.biortech.2019.01.080
Publication status	Published - 2019 Apr

Bibliographical note

Funding Information:
The authors are grateful for the support of the Korea CCS R&D Center (Korea CCS 2020 Project) which was funded by the Korean Government (Ministry of Science and ICT) in 2018 (Grant Number: KCRC - 2014M1A8A1049278 ). The authors thank the National Research Foundation of Korea (NRF) (Grant Number: NRF-2016R1A2A1A05005465) for support, and also gratefully acknowledge the support of the Korea Institute of Energy Technology Evaluation and Planning (KETEP), which was funded by the Korean Government ( Ministry of Trade, Industry and Energy ) (Grant Number: 20172010202050 ).

Funding Information:
The authors are grateful for the support of the Korea CCS R&D Center (Korea CCS 2020 Project) which was funded by the Korean Government (Ministry of Science and ICT) in 2018 (Grant Number: KCRC-2014M1A8A1049278). The authors thank the National Research Foundation of Korea (NRF) (Grant Number: NRF-2016R1A2A1A05005465) for support, and also gratefully acknowledge the support of the Korea Institute of Energy Technology Evaluation and Planning (KETEP), which was funded by the Korean Government (Ministry of Trade, Industry and Energy) (Grant Number: 20172010202050).

Publisher Copyright:
© 2019 Elsevier Ltd

Keywords

Acidic cultivation
Astaxanthin
Haematococcus pluvialis
Paraphysoderma sedebokerensis
Stress relief

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2019.01.080

Cite this

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title = "Acidic cultivation of Haematococcus pluvialis for improved astaxanthin production in the presence of a lethal fungus",

abstract = "An acidic cultivation strategy was developed to prevent contamination of a lethal fungus Paraphysoderma sedebokerensis in Haematococcus pluvialis culture for astaxanthin production. Instead of generally used neutral pH, an acidic condition (pH 4) was applied to the cultivation, resulting in a significant inhibition of the fungal contamination. This could be ascribed to the acidity-associated denaturation of a surface protein of P. sedebokerensis, which plays an important role in recognition of H. pluvialis. Stress relief strategies including stepwise light irradiation and naturally occurring nitrogen deficiency were employed in the induction stage to minimize the reduction of astaxanthin production caused by acidic pH. Accordingly, an astaxanthin titer of 84.8 mg L−1 was obtained, which is 141-fold of that from the completely contaminated culture and double of that without the stress relief methods. This strategy provides a persistent contamination control method that can be used for practical astaxanthin production by H. pluvialis.",

keywords = "Acidic cultivation, Astaxanthin, Haematococcus pluvialis, Paraphysoderma sedebokerensis, Stress relief",

author = "Hwang, {Sung Won} and Choi, {Hong Il} and Sim, {Sang Jun}",

note = "Funding Information: The authors are grateful for the support of the Korea CCS R&D Center (Korea CCS 2020 Project) which was funded by the Korean Government (Ministry of Science and ICT) in 2018 (Grant Number: KCRC - 2014M1A8A1049278 ). The authors thank the National Research Foundation of Korea (NRF) (Grant Number: NRF-2016R1A2A1A05005465) for support, and also gratefully acknowledge the support of the Korea Institute of Energy Technology Evaluation and Planning (KETEP), which was funded by the Korean Government ( Ministry of Trade, Industry and Energy ) (Grant Number: 20172010202050 ). Funding Information: The authors are grateful for the support of the Korea CCS R&D Center (Korea CCS 2020 Project) which was funded by the Korean Government (Ministry of Science and ICT) in 2018 (Grant Number: KCRC-2014M1A8A1049278). The authors thank the National Research Foundation of Korea (NRF) (Grant Number: NRF-2016R1A2A1A05005465) for support, and also gratefully acknowledge the support of the Korea Institute of Energy Technology Evaluation and Planning (KETEP), which was funded by the Korean Government (Ministry of Trade, Industry and Energy) (Grant Number: 20172010202050). Publisher Copyright: {\textcopyright} 2019 Elsevier Ltd",

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AU - Hwang, Sung Won

AU - Choi, Hong Il

AU - Sim, Sang Jun

N1 - Funding Information: The authors are grateful for the support of the Korea CCS R&D Center (Korea CCS 2020 Project) which was funded by the Korean Government (Ministry of Science and ICT) in 2018 (Grant Number: KCRC - 2014M1A8A1049278 ). The authors thank the National Research Foundation of Korea (NRF) (Grant Number: NRF-2016R1A2A1A05005465) for support, and also gratefully acknowledge the support of the Korea Institute of Energy Technology Evaluation and Planning (KETEP), which was funded by the Korean Government ( Ministry of Trade, Industry and Energy ) (Grant Number: 20172010202050 ). Funding Information: The authors are grateful for the support of the Korea CCS R&D Center (Korea CCS 2020 Project) which was funded by the Korean Government (Ministry of Science and ICT) in 2018 (Grant Number: KCRC-2014M1A8A1049278). The authors thank the National Research Foundation of Korea (NRF) (Grant Number: NRF-2016R1A2A1A05005465) for support, and also gratefully acknowledge the support of the Korea Institute of Energy Technology Evaluation and Planning (KETEP), which was funded by the Korean Government (Ministry of Trade, Industry and Energy) (Grant Number: 20172010202050). Publisher Copyright: © 2019 Elsevier Ltd

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N2 - An acidic cultivation strategy was developed to prevent contamination of a lethal fungus Paraphysoderma sedebokerensis in Haematococcus pluvialis culture for astaxanthin production. Instead of generally used neutral pH, an acidic condition (pH 4) was applied to the cultivation, resulting in a significant inhibition of the fungal contamination. This could be ascribed to the acidity-associated denaturation of a surface protein of P. sedebokerensis, which plays an important role in recognition of H. pluvialis. Stress relief strategies including stepwise light irradiation and naturally occurring nitrogen deficiency were employed in the induction stage to minimize the reduction of astaxanthin production caused by acidic pH. Accordingly, an astaxanthin titer of 84.8 mg L−1 was obtained, which is 141-fold of that from the completely contaminated culture and double of that without the stress relief methods. This strategy provides a persistent contamination control method that can be used for practical astaxanthin production by H. pluvialis.

AB - An acidic cultivation strategy was developed to prevent contamination of a lethal fungus Paraphysoderma sedebokerensis in Haematococcus pluvialis culture for astaxanthin production. Instead of generally used neutral pH, an acidic condition (pH 4) was applied to the cultivation, resulting in a significant inhibition of the fungal contamination. This could be ascribed to the acidity-associated denaturation of a surface protein of P. sedebokerensis, which plays an important role in recognition of H. pluvialis. Stress relief strategies including stepwise light irradiation and naturally occurring nitrogen deficiency were employed in the induction stage to minimize the reduction of astaxanthin production caused by acidic pH. Accordingly, an astaxanthin titer of 84.8 mg L−1 was obtained, which is 141-fold of that from the completely contaminated culture and double of that without the stress relief methods. This strategy provides a persistent contamination control method that can be used for practical astaxanthin production by H. pluvialis.

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Acidic cultivation of Haematococcus pluvialis for improved astaxanthin production in the presence of a lethal fungus

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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