Activation of crosslinked lipases in mesoporous silica via lid opening for recyclable biodiesel production

Meiling Zhang, Seung Hyun Jun, Youngho Wee, Han Sol Kim, Ee Taek Hwang, Jongmin Shim, Sang Youn Hwang, Jinwoo Lee, Jungbae Kim

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)


Lipases catalyze a wide range of industrially important reactions, including the transesterification of triglycerides with alcohols for biodiesel production, and the stabilization of lipases are critical to achieve their recycled uses. Here, nanoscale enzyme reactor (NER) of lipase from Rhizopus oryzae (LP) was prepared via a simple two-step process, comprising of enzyme adsorption into magnetically-separable mesoporous silica and follow-up crosslinking of adsorbed enzymes. In aqueous phase, the specific hydrolysis activity of NER-LP was 4.7 times lower than that of free LP. On the other hand, however, the specific transesterification activity of NER-LP (130.4 μmol/min/mg LP) in organic phase for biodiesel production was 50 times higher than that of free LP (2.6 μmol/min/mg LP). These results reveal that the enzyme crosslinking for the preparation of NER does not interfere with the interfacial activation of LP molecules, opening the lid of LP active site under an optimal hydrophobic environment provided by the combination of organic solvent and mesoporous silica. Magnetic separation and optimized washing protocol facilitated the recycled uses of NER-LP. Highly stable and active NER-LP in magnetically-separable mesoporous silica has demonstrated its great potentials as an environmentally-friendly nanobiocatalyst for various lipase applications, including plasticizers, biosurfactants, functional fatty acids, as well as recyclable biodiesel production.

Original languageEnglish
Pages (from-to)2368-2374
Number of pages7
JournalInternational Journal of Biological Macromolecules
Publication statusPublished - 2022 Dec 1

Bibliographical note

Publisher Copyright:
© 2022 Elsevier B.V.


  • Enzyme stabilization
  • Lipase lid opening
  • Lipase-catalyzed transesterification
  • Mesoporous silica
  • Nanoscale enzyme reactors

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology


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