Activation of natural killer T cells inhibits the development of induced regulatory T cells via IFNγ

Kyu Heon Oh; Changjin Lee; Sung Won Lee; Sung H. Jeon; Se Ho Park; Rho H. Seong; Seokmann Hong

doi:10.1016/j.bbrc.2011.06.193

Activation of natural killer T cells inhibits the development of induced regulatory T cells via IFNγ

Kyu Heon Oh, Changjin Lee, Sung Won Lee, Sung H. Jeon, Se Ho Park, Rho H. Seong, Seokmann Hong

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Recent reports have provided evidence for cross-talk between regulatory T (Treg) cells and natural killer T (NKT) cells. However, it is unclear whether NKT cells play a role in the differentiation of Treg cells. By employing NKT cell-abundant Vα14 TCR transgenic (Tg) and NKT cell-deficient CD1d knock-out (KO) mice, we examined the effects of NKT cells on the in vitro differentiation of induced Treg (iTreg) cells with IL2 and TGFβ. We found that iTreg induction from CD1d KO mice was significantly increased compared to the control. Also, the addition of isolated NKT cells from Vα14 TCR Tg mice to naïve CD4⁺ T cells from CD1d KO mice during iTreg differentiation caused a remarkable reduction of iTreg cells. Through IFNγ neutralization, we showed that this reduction was mediated by IFNγ. Furthermore, the main source of IFNγ during iTreg differentiation was NK1.1^-CD4⁺Foxp3^- T cells. This finding implied that early-activated NKT cells induced Th1-type cells and subsequently underwent apoptosis. Taken together, our results suggest that NKT cells inhibit the in vitro development of iTreg cells by increasing IFNγ.

Original language	English
Pages (from-to)	599-606
Number of pages	8
Journal	Biochemical and biophysical research communications
Volume	411
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2011.06.193
Publication status	Published - 2011 Aug 5

Bibliographical note

Funding Information:
This study was supported by a grant from the National R&D Program for Cancer Control , Ministry for Health , Welfare and Family affairs , Republic of Korea ( #0820270 ).

Keywords

Interferon gamma
Natural killer T cell
Regulatory T cell

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/j.bbrc.2011.06.193

Cite this

@article{4c16dedcb88c45e39259c9d0ce266f10,

title = "Activation of natural killer T cells inhibits the development of induced regulatory T cells via IFNγ",

abstract = "Recent reports have provided evidence for cross-talk between regulatory T (Treg) cells and natural killer T (NKT) cells. However, it is unclear whether NKT cells play a role in the differentiation of Treg cells. By employing NKT cell-abundant Vα14 TCR transgenic (Tg) and NKT cell-deficient CD1d knock-out (KO) mice, we examined the effects of NKT cells on the in vitro differentiation of induced Treg (iTreg) cells with IL2 and TGFβ. We found that iTreg induction from CD1d KO mice was significantly increased compared to the control. Also, the addition of isolated NKT cells from Vα14 TCR Tg mice to na{\"i}ve CD4+ T cells from CD1d KO mice during iTreg differentiation caused a remarkable reduction of iTreg cells. Through IFNγ neutralization, we showed that this reduction was mediated by IFNγ. Furthermore, the main source of IFNγ during iTreg differentiation was NK1.1-CD4+Foxp3- T cells. This finding implied that early-activated NKT cells induced Th1-type cells and subsequently underwent apoptosis. Taken together, our results suggest that NKT cells inhibit the in vitro development of iTreg cells by increasing IFNγ.",

keywords = "Interferon gamma, Natural killer T cell, Regulatory T cell",

author = "Oh, {Kyu Heon} and Changjin Lee and Lee, {Sung Won} and Jeon, {Sung H.} and Park, {Se Ho} and Seong, {Rho H.} and Seokmann Hong",

note = "Funding Information: This study was supported by a grant from the National R&D Program for Cancer Control , Ministry for Health , Welfare and Family affairs , Republic of Korea ( #0820270 ).",

year = "2011",

month = aug,

day = "5",

doi = "10.1016/j.bbrc.2011.06.193",

language = "English",

volume = "411",

pages = "599--606",

journal = "Biochemical and biophysical research communications",

issn = "0006-291X",

publisher = "Elsevier B.V.",

number = "3",

}

TY - JOUR

T1 - Activation of natural killer T cells inhibits the development of induced regulatory T cells via IFNγ

AU - Oh, Kyu Heon

AU - Lee, Changjin

AU - Lee, Sung Won

AU - Jeon, Sung H.

AU - Park, Se Ho

AU - Seong, Rho H.

AU - Hong, Seokmann

N1 - Funding Information: This study was supported by a grant from the National R&D Program for Cancer Control , Ministry for Health , Welfare and Family affairs , Republic of Korea ( #0820270 ).

PY - 2011/8/5

Y1 - 2011/8/5

N2 - Recent reports have provided evidence for cross-talk between regulatory T (Treg) cells and natural killer T (NKT) cells. However, it is unclear whether NKT cells play a role in the differentiation of Treg cells. By employing NKT cell-abundant Vα14 TCR transgenic (Tg) and NKT cell-deficient CD1d knock-out (KO) mice, we examined the effects of NKT cells on the in vitro differentiation of induced Treg (iTreg) cells with IL2 and TGFβ. We found that iTreg induction from CD1d KO mice was significantly increased compared to the control. Also, the addition of isolated NKT cells from Vα14 TCR Tg mice to naïve CD4+ T cells from CD1d KO mice during iTreg differentiation caused a remarkable reduction of iTreg cells. Through IFNγ neutralization, we showed that this reduction was mediated by IFNγ. Furthermore, the main source of IFNγ during iTreg differentiation was NK1.1-CD4+Foxp3- T cells. This finding implied that early-activated NKT cells induced Th1-type cells and subsequently underwent apoptosis. Taken together, our results suggest that NKT cells inhibit the in vitro development of iTreg cells by increasing IFNγ.

AB - Recent reports have provided evidence for cross-talk between regulatory T (Treg) cells and natural killer T (NKT) cells. However, it is unclear whether NKT cells play a role in the differentiation of Treg cells. By employing NKT cell-abundant Vα14 TCR transgenic (Tg) and NKT cell-deficient CD1d knock-out (KO) mice, we examined the effects of NKT cells on the in vitro differentiation of induced Treg (iTreg) cells with IL2 and TGFβ. We found that iTreg induction from CD1d KO mice was significantly increased compared to the control. Also, the addition of isolated NKT cells from Vα14 TCR Tg mice to naïve CD4+ T cells from CD1d KO mice during iTreg differentiation caused a remarkable reduction of iTreg cells. Through IFNγ neutralization, we showed that this reduction was mediated by IFNγ. Furthermore, the main source of IFNγ during iTreg differentiation was NK1.1-CD4+Foxp3- T cells. This finding implied that early-activated NKT cells induced Th1-type cells and subsequently underwent apoptosis. Taken together, our results suggest that NKT cells inhibit the in vitro development of iTreg cells by increasing IFNγ.

KW - Interferon gamma

KW - Natural killer T cell

KW - Regulatory T cell

UR - http://www.scopus.com/inward/record.url?scp=79961128262&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2011.06.193

DO - 10.1016/j.bbrc.2011.06.193

M3 - Article

C2 - 21763281

AN - SCOPUS:79961128262

SN - 0006-291X

VL - 411

SP - 599

EP - 606

JO - Biochemical and biophysical research communications

JF - Biochemical and biophysical research communications

IS - 3

ER -

Activation of natural killer T cells inhibits the development of induced regulatory T cells via IFNγ

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this