Activation of peripheral group III metabotropic glutamate receptors inhibits pain transmission by decreasing neuronal excitability in the CFA-inflamed knee joint

Eui Ho Park; Seung Won Lee; Sun Wook Moon; Hye Rim Suh; Yang In Kim; Hee Chul Han

doi:10.1016/j.neulet.2018.11.033

Activation of peripheral group III metabotropic glutamate receptors inhibits pain transmission by decreasing neuronal excitability in the CFA-inflamed knee joint

Eui Ho Park, Seung Won Lee, Sun Wook Moon, Hye Rim Suh, Yang In Kim, Hee Chul Han

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Peripheral group III metabotropic glutamate receptors (mGluRs) function to modulate pain signaling in inflammatory states. Here, we established in vivo experimental settings, including dynamic weight bearing test and in vivo single nerve recording, to elucidate how the group III mGluRs contribute to inhibiting pain transmission at the peripheral sensory nerve terminal in inflammatory states (1 and 3 days) elicited by Complete Freund's Adjuvant (CFA). As a result, CFA-induced nociceptive behaviors were significantly alleviated after administration of 100 and 200 μM L-AP4 (l-2-amino-4-phosphonobutylate; group III mGluR agonist). In addition, neuronal discharges evoked by 6- and 26-g von Frey filaments at the nerve significantly decreased after administration of 200 μM L-AP4. However, this event was not observed in non-inflammatory state. These results suggest that the group III mGluRs negatively regulate nociceptive behavior and pain transmission by lessening neuronal firing rates at the peripheral nerve in inflammation.

Original language	English
Pages (from-to)	111-115
Number of pages	5
Journal	Neuroscience Letters
Volume	694
DOIs	https://doi.org/10.1016/j.neulet.2018.11.033
Publication status	Published - 2019 Feb 16

Bibliographical note

Funding Information:
This work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2016R1D1A1B03933951), and partially supported by Korea University grant (K1813201), Korea University Research Fellowship Grant Program, and the Chung Yang, Cha Young Sun & Jang Hi Joo Memorial Fund (20140101).

Funding Information:
This work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education ( 2016R1D1A1B03933951 ), and partially supported by Korea University grant ( K1813201 ), Korea University Research Fellowship Grant Program , and the Chung Yang, Cha Young Sun & Jang Hi Joo Memorial Fund ( 20140101 ).

Publisher Copyright:
© 2018

Keywords

CFA
Dynamic weight bearing
Group III mGluR
In vivo single nerve recording
Knee joint
Pain transmission

ASJC Scopus subject areas

General Neuroscience

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10.1016/j.neulet.2018.11.033

Cite this

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title = "Activation of peripheral group III metabotropic glutamate receptors inhibits pain transmission by decreasing neuronal excitability in the CFA-inflamed knee joint",

abstract = "Peripheral group III metabotropic glutamate receptors (mGluRs) function to modulate pain signaling in inflammatory states. Here, we established in vivo experimental settings, including dynamic weight bearing test and in vivo single nerve recording, to elucidate how the group III mGluRs contribute to inhibiting pain transmission at the peripheral sensory nerve terminal in inflammatory states (1 and 3 days) elicited by Complete Freund's Adjuvant (CFA). As a result, CFA-induced nociceptive behaviors were significantly alleviated after administration of 100 and 200 μM L-AP4 (l-2-amino-4-phosphonobutylate; group III mGluR agonist). In addition, neuronal discharges evoked by 6- and 26-g von Frey filaments at the nerve significantly decreased after administration of 200 μM L-AP4. However, this event was not observed in non-inflammatory state. These results suggest that the group III mGluRs negatively regulate nociceptive behavior and pain transmission by lessening neuronal firing rates at the peripheral nerve in inflammation.",

keywords = "CFA, Dynamic weight bearing, Group III mGluR, In vivo single nerve recording, Knee joint, Pain transmission",

author = "Park, {Eui Ho} and Lee, {Seung Won} and Moon, {Sun Wook} and Suh, {Hye Rim} and Kim, {Yang In} and Han, {Hee Chul}",

note = "Funding Information: This work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2016R1D1A1B03933951), and partially supported by Korea University grant (K1813201), Korea University Research Fellowship Grant Program, and the Chung Yang, Cha Young Sun & Jang Hi Joo Memorial Fund (20140101). Funding Information: This work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education ( 2016R1D1A1B03933951 ), and partially supported by Korea University grant ( K1813201 ), Korea University Research Fellowship Grant Program , and the Chung Yang, Cha Young Sun & Jang Hi Joo Memorial Fund ( 20140101 ). Publisher Copyright: {\textcopyright} 2018",

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AU - Moon, Sun Wook

AU - Suh, Hye Rim

AU - Kim, Yang In

AU - Han, Hee Chul

N1 - Funding Information: This work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2016R1D1A1B03933951), and partially supported by Korea University grant (K1813201), Korea University Research Fellowship Grant Program, and the Chung Yang, Cha Young Sun & Jang Hi Joo Memorial Fund (20140101). Funding Information: This work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education ( 2016R1D1A1B03933951 ), and partially supported by Korea University grant ( K1813201 ), Korea University Research Fellowship Grant Program , and the Chung Yang, Cha Young Sun & Jang Hi Joo Memorial Fund ( 20140101 ). Publisher Copyright: © 2018

PY - 2019/2/16

Y1 - 2019/2/16

N2 - Peripheral group III metabotropic glutamate receptors (mGluRs) function to modulate pain signaling in inflammatory states. Here, we established in vivo experimental settings, including dynamic weight bearing test and in vivo single nerve recording, to elucidate how the group III mGluRs contribute to inhibiting pain transmission at the peripheral sensory nerve terminal in inflammatory states (1 and 3 days) elicited by Complete Freund's Adjuvant (CFA). As a result, CFA-induced nociceptive behaviors were significantly alleviated after administration of 100 and 200 μM L-AP4 (l-2-amino-4-phosphonobutylate; group III mGluR agonist). In addition, neuronal discharges evoked by 6- and 26-g von Frey filaments at the nerve significantly decreased after administration of 200 μM L-AP4. However, this event was not observed in non-inflammatory state. These results suggest that the group III mGluRs negatively regulate nociceptive behavior and pain transmission by lessening neuronal firing rates at the peripheral nerve in inflammation.

AB - Peripheral group III metabotropic glutamate receptors (mGluRs) function to modulate pain signaling in inflammatory states. Here, we established in vivo experimental settings, including dynamic weight bearing test and in vivo single nerve recording, to elucidate how the group III mGluRs contribute to inhibiting pain transmission at the peripheral sensory nerve terminal in inflammatory states (1 and 3 days) elicited by Complete Freund's Adjuvant (CFA). As a result, CFA-induced nociceptive behaviors were significantly alleviated after administration of 100 and 200 μM L-AP4 (l-2-amino-4-phosphonobutylate; group III mGluR agonist). In addition, neuronal discharges evoked by 6- and 26-g von Frey filaments at the nerve significantly decreased after administration of 200 μM L-AP4. However, this event was not observed in non-inflammatory state. These results suggest that the group III mGluRs negatively regulate nociceptive behavior and pain transmission by lessening neuronal firing rates at the peripheral nerve in inflammation.

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Activation of peripheral group III metabotropic glutamate receptors inhibits pain transmission by decreasing neuronal excitability in the CFA-inflamed knee joint

Abstract

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Keywords

ASJC Scopus subject areas

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