Activity-based fluorescence probes for pathophysiological peroxynitrite fluxes

Zhiqiang Mao, Jianhua Xiong, Pengzhan Wang, Jusung An, Fan Zhang, Zhihong Liu, Jong Seung Kim

Research output: Contribution to journalReview articlepeer-review

67 Citations (Scopus)


Peroxynitrite (ONOO), one of the reactive oxygen and nitrogen species (RONS), is famous for its potent oxidation and nitration capabilities and plays crucial roles in various pathological conditions. Given the similar chemical properties of various RONS (e.g. short half-life time, ultralow content, and mutual interconversion), specific detecting of ONOO over other RONS remains a challenging mission in the complex physiological environment, primarily ONOO related pathological processes. To decipher the intricate associations, molecular imaging assisted by activity-based sensing (ABS) fluorescent probes has become the “state-of-art” method due to its superiorities, including susceptible and selective detection results, non-invasive and real-time visualization. This review focuses on the research of ABS fluorescent probes that revealed the vital roles of ONOO in various diseases, such as inflammations, tumors, liver injuries, and brain diseases. Finally, we discuss the bottlenecks and future developing trends for ABS probes which will boost ABS probes’ applications in chemistry, biology, and pharmacology.

Original languageEnglish
Article number214356
JournalCoordination Chemistry Reviews
Publication statusPublished - 2022 Mar 1

Bibliographical note

Funding Information:
This work was financially supported by the National Natural Science Foundation of China (Nos. 22174034 , 21804033 , 21625503 , and 21904031 ), the Foundation for Creative Research Groups of Hubei Province of China (No. 2020CFA035), the Natural Science Foundation of Hubei Province ( 2021CFB305 ), and funded by the National Research Foundation of Korea (CRI project No. 2018R1A3B1052702, NRF-2019M3E5D1A01068998, J. S. Kim) and the Korea University Graduate School Junior Fellow Research Grant (J. An).

Publisher Copyright:
© 2021


  • Activity-based sensing
  • Fluorescent probe
  • Inflammation
  • Peroxynitrite
  • Tumor

ASJC Scopus subject areas

  • Materials Chemistry
  • General Chemistry
  • Inorganic Chemistry
  • Physical and Theoretical Chemistry


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