TY - JOUR
T1 - Activity of crude and fractionated extracts by lactic acid bacteria (LAB) isolated from local dairy, meat, and fermented products against Staphylococcus aureus
AU - Yong, Cheng Chung
AU - Khoo, Boon Yin
AU - Sasidharan, Sreenivasan
AU - Piyawattanametha, Wibool
AU - Kim, Sae Hun
AU - Khemthongcharoen, Numfon
AU - Chuah, Li Oon
AU - Ang, May Yen
AU - Liong, Min Tze
N1 - Funding Information:
Acknowledgments This work was financially supported by the Science Fund Grant (305/PTEKIND/613222) provided by the Malaysian Ministry of Science, Technology, and Innovation (MOSTI), the FRGS grant (203/PTEKIND/6711239) provided by the Malaysian Ministry of Higher Education (MOHE), USM RU grants (1001/PKIMIA/855006, 1001/PTEKIND/815085), and USM Fellowship provided by Universiti Sains Malaysia.
Publisher Copyright:
© 2014, Springer-Verlag Berlin Heidelberg and the University of Milan.
PY - 2015/6/26
Y1 - 2015/6/26
N2 - This study aimed to evaluate anti-staphylococcal properties of crude and fractionated extracts of lactic acid bacteria (LAB) isolated from local meat, dairy, and fermented products. A total of 36 LAB isolates were obtained and identified via 16S rDNA sequencing. Cell-free supernatant (CFS) of all isolates exhibiting a statistically significant inhibition against Staphylococcus aureus (ρ < 0.05), with six LAB isolates exhibiting a more prevalent inhibition. The inhibition effects of cell wall and intracellular extracts from the six prevalent isolates were evaluated. Lactobacillus plantarum USM8613 was the most prominent isolate with both CFS and cell wall extract exhibiting the most prevalent inhibition against S. aureus. Scanning electron micrographs showed alteration of S. aureus membrane morphology upon CFS treatment, suggesting an anti-staphylococcal effect via membrane destruction. Confocal laser scanning micrographs showed inhibition against biofilm formations by S. aureus in porcine skins upon CFS treatment. The CFS from L. plantarum USM8613 was separated into protein, lipid, and polysaccharide fractions for evaluation of anti-staphylococcal activity and chemical characterization. All fractions inhibited growth of S. aureus (ρ < 0.05), with protein fractions exhibiting stronger inhibition effect. Data from our present study showed that extracts from LAB could be applied as biopreservatives in the food industries and/or as an antimicrobial agent against bacterial infections for cosmeceutical and pharmaceutical uses.
AB - This study aimed to evaluate anti-staphylococcal properties of crude and fractionated extracts of lactic acid bacteria (LAB) isolated from local meat, dairy, and fermented products. A total of 36 LAB isolates were obtained and identified via 16S rDNA sequencing. Cell-free supernatant (CFS) of all isolates exhibiting a statistically significant inhibition against Staphylococcus aureus (ρ < 0.05), with six LAB isolates exhibiting a more prevalent inhibition. The inhibition effects of cell wall and intracellular extracts from the six prevalent isolates were evaluated. Lactobacillus plantarum USM8613 was the most prominent isolate with both CFS and cell wall extract exhibiting the most prevalent inhibition against S. aureus. Scanning electron micrographs showed alteration of S. aureus membrane morphology upon CFS treatment, suggesting an anti-staphylococcal effect via membrane destruction. Confocal laser scanning micrographs showed inhibition against biofilm formations by S. aureus in porcine skins upon CFS treatment. The CFS from L. plantarum USM8613 was separated into protein, lipid, and polysaccharide fractions for evaluation of anti-staphylococcal activity and chemical characterization. All fractions inhibited growth of S. aureus (ρ < 0.05), with protein fractions exhibiting stronger inhibition effect. Data from our present study showed that extracts from LAB could be applied as biopreservatives in the food industries and/or as an antimicrobial agent against bacterial infections for cosmeceutical and pharmaceutical uses.
KW - Antimicrobials
KW - Lactic acid bacteria
KW - Lactobacillus
KW - Staphylococcus aureus
UR - http://www.scopus.com/inward/record.url?scp=84929836035&partnerID=8YFLogxK
U2 - 10.1007/s13213-014-0949-1
DO - 10.1007/s13213-014-0949-1
M3 - Article
AN - SCOPUS:84929836035
SN - 1590-4261
VL - 65
SP - 1037
EP - 1047
JO - Annals of Microbiology
JF - Annals of Microbiology
IS - 2
ER -