Adaptor protein GULP is involved in stabilin-1-mediated phagocytosis

Seung Yoon Park, Sang Yeob Kim, Kae Bok Kang, In San Kim

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

The clearance of apoptotic cells is critical during cellular homeostasis as well as inflammation resolution. Recently, we found that stabilin-1 is a phagocytic receptor that is involved in the clearance of apoptotic cells. However, the downstream signaling pathway of stabilin-1-mediated phagocytosis remains to be investigated. Here we identify that GULP is able to specifically interact with the NPxF/Y motif of stabilin-1 cytoplasmic region. The PTB domain of GULP is necessary for interaction with stabilin-1. GULP is enriched around PS-coated beads for phagocytosis and co-localized with stabilin-1. Downregulation of endogenous GULP expression decreased stabilin-1-mediated phagocytosis. Thus, these results indicate that GULP functions as an adaptor protein for stabilin-1-mediated phagocytosis.

Original languageEnglish
Pages (from-to)467-472
Number of pages6
JournalBiochemical and biophysical research communications
Volume398
Issue number3
DOIs
Publication statusPublished - 2010 Jul
Externally publishedYes

Bibliographical note

Funding Information:
This study was supported by Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology ( R11-2008-044-03001-0 ); by National Research Foundation of Korea Grant funded by the Korean Government ( 2009-0068687 ); by WCU (World Class University) program through the Korea Science and Engineering Foundation funded by the Ministry of Education, Science and Technology ( R33-2008-000-10054-0 ); by the Grant no. RTI04-01-01 from the Regional Technology Innovation Program of the Ministry of Knowledge Economy (MKE) ; and by the Brain Korea 21 Project .

Keywords

  • Aged RBC
  • GULP
  • Phagocytosis
  • Phosphatidylserine
  • Stabilin-1

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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