Adenosine-mediated synaptic depression and EPSP/spike dissociation following high potassium-induced depolarization in rat hippocampal slices

Youn Kwan Park; Eun Sheb Shim; Jae In Oh; Joo Han Kim; Yong Gu Chung

doi:10.1016/S0006-8993(03)02628-3

Adenosine-mediated synaptic depression and EPSP/spike dissociation following high potassium-induced depolarization in rat hippocampal slices

Youn Kwan Park, Eun Sheb Shim, Jae In Oh, Joo Han Kim, Yong Gu Chung

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Simultaneous recordings of orthodromic PS, fEPSP and antidromic PS revealed EPSP/spike (E-S) dissociation, indicating a conversion of input/output relations from early and brief excitability to a late and prolonged depression during the recovery from depolarization induced by high levels of potassium. E-S potentiation was partially attenuated by pre-treating the slices with BAPTA-AM and lidocaine and totally eliminated by a submaximal concentration of muscimol. The time lag for recovery was decreased by the GABA_A antagonist and completely eliminated by the A₁ antagonist. From these observations, we conclude that Ca²⁺ dependent inhibitory suppression is the main cause of a brief period of E-S potentiation, and accumulation of adenosine is the mechanism responsible for prolonged depression of synaptic transmission.

Original language	English
Pages (from-to)	237-243
Number of pages	7
Journal	Brain Research
Volume	975
Issue number	1-2
DOIs	https://doi.org/10.1016/S0006-8993(03)02628-3
Publication status	Published - 2003 Jun 13
Externally published	Yes

Bibliographical note

Funding Information:
This study was supported by the Brain Korea 21 project in 2002 and a grant to Dr Youn-Kwan Park from the Korea University Medical Science Research Center (Grant No. HO311000). All procedures were in accordance with guidelines outlined by the Institutional Animal care and Use Committee of Korea University.

Keywords

Depolarization
EPSP/spike dissociation
High K irrigation
Hippocampus

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/S0006-8993(03)02628-3

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title = "Adenosine-mediated synaptic depression and EPSP/spike dissociation following high potassium-induced depolarization in rat hippocampal slices",

abstract = "Simultaneous recordings of orthodromic PS, fEPSP and antidromic PS revealed EPSP/spike (E-S) dissociation, indicating a conversion of input/output relations from early and brief excitability to a late and prolonged depression during the recovery from depolarization induced by high levels of potassium. E-S potentiation was partially attenuated by pre-treating the slices with BAPTA-AM and lidocaine and totally eliminated by a submaximal concentration of muscimol. The time lag for recovery was decreased by the GABAA antagonist and completely eliminated by the A1 antagonist. From these observations, we conclude that Ca2+ dependent inhibitory suppression is the main cause of a brief period of E-S potentiation, and accumulation of adenosine is the mechanism responsible for prolonged depression of synaptic transmission.",

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author = "Park, {Youn Kwan} and Shim, {Eun Sheb} and Oh, {Jae In} and Kim, {Joo Han} and Chung, {Yong Gu}",

note = "Funding Information: This study was supported by the Brain Korea 21 project in 2002 and a grant to Dr Youn-Kwan Park from the Korea University Medical Science Research Center (Grant No. HO311000). All procedures were in accordance with guidelines outlined by the Institutional Animal care and Use Committee of Korea University.",

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AU - Park, Youn Kwan

AU - Shim, Eun Sheb

AU - Oh, Jae In

AU - Kim, Joo Han

AU - Chung, Yong Gu

N1 - Funding Information: This study was supported by the Brain Korea 21 project in 2002 and a grant to Dr Youn-Kwan Park from the Korea University Medical Science Research Center (Grant No. HO311000). All procedures were in accordance with guidelines outlined by the Institutional Animal care and Use Committee of Korea University.

PY - 2003/6/13

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N2 - Simultaneous recordings of orthodromic PS, fEPSP and antidromic PS revealed EPSP/spike (E-S) dissociation, indicating a conversion of input/output relations from early and brief excitability to a late and prolonged depression during the recovery from depolarization induced by high levels of potassium. E-S potentiation was partially attenuated by pre-treating the slices with BAPTA-AM and lidocaine and totally eliminated by a submaximal concentration of muscimol. The time lag for recovery was decreased by the GABAA antagonist and completely eliminated by the A1 antagonist. From these observations, we conclude that Ca2+ dependent inhibitory suppression is the main cause of a brief period of E-S potentiation, and accumulation of adenosine is the mechanism responsible for prolonged depression of synaptic transmission.

AB - Simultaneous recordings of orthodromic PS, fEPSP and antidromic PS revealed EPSP/spike (E-S) dissociation, indicating a conversion of input/output relations from early and brief excitability to a late and prolonged depression during the recovery from depolarization induced by high levels of potassium. E-S potentiation was partially attenuated by pre-treating the slices with BAPTA-AM and lidocaine and totally eliminated by a submaximal concentration of muscimol. The time lag for recovery was decreased by the GABAA antagonist and completely eliminated by the A1 antagonist. From these observations, we conclude that Ca2+ dependent inhibitory suppression is the main cause of a brief period of E-S potentiation, and accumulation of adenosine is the mechanism responsible for prolonged depression of synaptic transmission.

KW - Depolarization

KW - EPSP/spike dissociation

KW - High K irrigation

KW - Hippocampus

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Adenosine-mediated synaptic depression and EPSP/spike dissociation following high potassium-induced depolarization in rat hippocampal slices

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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