Adsorption of microbial esterases on Bacillus subtilis-templated cobalt oxide nanoparticles

Eunjin Jang, Bum Han Ryu, Hyun Woo Shim, Hansol Ju, Dong Wan Kim, T. Doohun Kim

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)


Due to low diffusion rates and large surface areas, nanomaterials have received great interest as supporting materials for enzyme immobilization. Here, the preparation of a cobalt oxide nanoparticle using Bacillus subtilis as a biological template and use of the nanostructure for microbial esterase immobilization is described. Morphological features and size distributions were investigated using electron microscopy (EM) and dynamic light scattering (DLS). Catalytic properties of enzyme-coated nanostructures were investigated using 4-methylumbelliferyl acetate and p-nitrophenyl (PNP) acetate as model substrates. Enzyme-coated nanostructures were observed to retain ~85% of the initial activity after 15 successive reaction cycles, and enzyme immobilization processes could be repeated four times without a loss of immobilization potential. The present work demonstrates that B. subtilis-templated cobalt oxide nanoparticles have the potential to be used as biocompatible immobilization materials, and are promising candidates for the preparation of effective nanobiocatalysts.

Original languageEnglish
Pages (from-to)188-192
Number of pages5
JournalInternational Journal of Biological Macromolecules
Publication statusPublished - 2014 Apr
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by National Research Foundation of Korea Grant funded by the Korean Government (NRF- 2012S1A2A1A01028907 , NRF- 2013K2A1A2053659 , NRF- 2012R1A1A2000910 ) to T.D.K., and (NRF- 2012R1A2A2A01045382 ) to D.W.K.


  • Bacillus subtilis
  • Cobalt-oxide nanoparticles
  • Hydrolase

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Economics and Econometrics
  • General Energy


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