Abstract
We have previously demonstrated that albumin is directly involved in the formation of cytoplasmic lipid droplets in pancreatic stellate cells and may act as a downstream effector of adipogenic transcription factors, PPAR-γ and C/EBP-α. Here, we investigated the role of albumin in adipocyte differentiation using 3T3-L1 cells. Albumin expression was significantly increased at later stages of adipocyte differentiation, which was accompanied with increased C/EBP-β binding to albumin promoter. Suppression of albumin expression using short-hairpin RNA (shRNA) during differentiation led to a considerable reduction in lipid droplet formation, whereas albumin overexpression was stimulatory. Furthermore, point mutation in its fatty acid-binding sites inhibited lipid droplet formation. Consistent with these in vitro finding, Nagase analbuminemic rats displayed reduced fat accumulation. Therefore, our findings suggest that albumin may play a distinct role in adipocyte differentiation by promoting lipid accumulation.
Original language | English |
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Pages (from-to) | 170-175 |
Number of pages | 6 |
Journal | Biochemical and biophysical research communications |
Volume | 397 |
Issue number | 2 |
DOIs | |
Publication status | Published - 2010 Jun 25 |
Bibliographical note
Funding Information:We thank Kyunghee Noh and Daewui Yoon for technical assistance and Dr. Jaebum Kim for generous support. This work was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea Government (MOST) (No. 2009-0076453 ).
Keywords
- 3T3-L1 adipocyte
- Adipogenesis
- Albumin
- C/EBP-α
- Lipid droplet formation
- PPAR-γ
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology