Albumin expression is required for adipocyte differentiation of 3T3-L1 cells

Wonbaek Yoo, Jaeseob Lee, Sangeun Park, Young Sik Kim, Chaeseung Lim, Eulsik Yoon, Gangmin Hur, Junseo Oh

    Research output: Contribution to journalArticlepeer-review

    14 Citations (Scopus)

    Abstract

    We have previously demonstrated that albumin is directly involved in the formation of cytoplasmic lipid droplets in pancreatic stellate cells and may act as a downstream effector of adipogenic transcription factors, PPAR-γ and C/EBP-α. Here, we investigated the role of albumin in adipocyte differentiation using 3T3-L1 cells. Albumin expression was significantly increased at later stages of adipocyte differentiation, which was accompanied with increased C/EBP-β binding to albumin promoter. Suppression of albumin expression using short-hairpin RNA (shRNA) during differentiation led to a considerable reduction in lipid droplet formation, whereas albumin overexpression was stimulatory. Furthermore, point mutation in its fatty acid-binding sites inhibited lipid droplet formation. Consistent with these in vitro finding, Nagase analbuminemic rats displayed reduced fat accumulation. Therefore, our findings suggest that albumin may play a distinct role in adipocyte differentiation by promoting lipid accumulation.

    Original languageEnglish
    Pages (from-to)170-175
    Number of pages6
    JournalBiochemical and biophysical research communications
    Volume397
    Issue number2
    DOIs
    Publication statusPublished - 2010 Jun 25

    Bibliographical note

    Funding Information:
    We thank Kyunghee Noh and Daewui Yoon for technical assistance and Dr. Jaebum Kim for generous support. This work was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea Government (MOST) (No. 2009-0076453 ).

    Keywords

    • 3T3-L1 adipocyte
    • Adipogenesis
    • Albumin
    • C/EBP-α
    • Lipid droplet formation
    • PPAR-γ

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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