Alendronate-eluting biphasic calcium phosphate (BCP) scaffolds stimulate osteogenic differentiation

Sung Eun Kim; Young Pil Yun; Deok Won Lee; Eun-Young Kang; Won Jae Jeong; Boram Lee; Myeong Seon Jeong; Hak Jun Kim; Kyeongsoon Park; Hae Ryong Song

doi:10.1155/2015/320713

Alendronate-eluting biphasic calcium phosphate (BCP) scaffolds stimulate osteogenic differentiation

Sung Eun Kim, Young Pil Yun, Deok Won Lee, Eun-Young Kang, Won Jae Jeong, Boram Lee, Myeong Seon Jeong, Hak Jun Kim, Kyeongsoon Park, Hae Ryong Song

Department of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

Biphasic calcium phosphate (BCP) scaffolds have been widely used in orthopedic and dental fields as osteoconductive bone substitutes. However, BCP scaffolds are not satisfactory for the stimulation of osteogenic differentiation and maturation. To enhance osteogenic differentiation, we prepared alendronate- (ALN-) eluting BCP scaffolds. The coating of ALN on BCP scaffolds was confirmed by scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDS), and attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR). An in vitro release study showed that release of ALN from ALN-eluting BCP scaffolds was sustained for up to 28 days. In vitro results revealed that MG-63 cells grown on ALN-eluting BCP scaffolds exhibited increased ALP activity and calcium deposition and upregulated gene expression of Runx2, ALP, OCN, and OPN compared with the BCP scaffold alone. Therefore, this study suggests that ALN-eluting BCP scaffolds have the potential to effectively stimulate osteogenic differentiation.

Original language	English
Article number	320713
Journal	BioMed Research International
Volume	2015
DOIs	https://doi.org/10.1155/2015/320713
Publication status	Published - 2015

Bibliographical note

Publisher Copyright:
© 2015 Sung Eun Kim et al.

ASJC Scopus subject areas

Immunology and Microbiology(all)
Biochemistry, Genetics and Molecular Biology(all)

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title = "Alendronate-eluting biphasic calcium phosphate (BCP) scaffolds stimulate osteogenic differentiation",

abstract = "Biphasic calcium phosphate (BCP) scaffolds have been widely used in orthopedic and dental fields as osteoconductive bone substitutes. However, BCP scaffolds are not satisfactory for the stimulation of osteogenic differentiation and maturation. To enhance osteogenic differentiation, we prepared alendronate- (ALN-) eluting BCP scaffolds. The coating of ALN on BCP scaffolds was confirmed by scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDS), and attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR). An in vitro release study showed that release of ALN from ALN-eluting BCP scaffolds was sustained for up to 28 days. In vitro results revealed that MG-63 cells grown on ALN-eluting BCP scaffolds exhibited increased ALP activity and calcium deposition and upregulated gene expression of Runx2, ALP, OCN, and OPN compared with the BCP scaffold alone. Therefore, this study suggests that ALN-eluting BCP scaffolds have the potential to effectively stimulate osteogenic differentiation.",

author = "Kim, {Sung Eun} and Yun, {Young Pil} and Lee, {Deok Won} and Eun-Young Kang and Jeong, {Won Jae} and Boram Lee and Jeong, {Myeong Seon} and Kim, {Hak Jun} and Kyeongsoon Park and Song, {Hae Ryong}",

note = "Publisher Copyright: {\textcopyright} 2015 Sung Eun Kim et al.",

year = "2015",

doi = "10.1155/2015/320713",

language = "English",

volume = "2015",

journal = "BioMed Research International",

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T1 - Alendronate-eluting biphasic calcium phosphate (BCP) scaffolds stimulate osteogenic differentiation

AU - Kim, Sung Eun

AU - Yun, Young Pil

AU - Lee, Deok Won

AU - Kang, Eun-Young

AU - Jeong, Won Jae

AU - Lee, Boram

AU - Jeong, Myeong Seon

AU - Kim, Hak Jun

AU - Park, Kyeongsoon

AU - Song, Hae Ryong

PY - 2015

Y1 - 2015

N2 - Biphasic calcium phosphate (BCP) scaffolds have been widely used in orthopedic and dental fields as osteoconductive bone substitutes. However, BCP scaffolds are not satisfactory for the stimulation of osteogenic differentiation and maturation. To enhance osteogenic differentiation, we prepared alendronate- (ALN-) eluting BCP scaffolds. The coating of ALN on BCP scaffolds was confirmed by scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDS), and attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR). An in vitro release study showed that release of ALN from ALN-eluting BCP scaffolds was sustained for up to 28 days. In vitro results revealed that MG-63 cells grown on ALN-eluting BCP scaffolds exhibited increased ALP activity and calcium deposition and upregulated gene expression of Runx2, ALP, OCN, and OPN compared with the BCP scaffold alone. Therefore, this study suggests that ALN-eluting BCP scaffolds have the potential to effectively stimulate osteogenic differentiation.

AB - Biphasic calcium phosphate (BCP) scaffolds have been widely used in orthopedic and dental fields as osteoconductive bone substitutes. However, BCP scaffolds are not satisfactory for the stimulation of osteogenic differentiation and maturation. To enhance osteogenic differentiation, we prepared alendronate- (ALN-) eluting BCP scaffolds. The coating of ALN on BCP scaffolds was confirmed by scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDS), and attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR). An in vitro release study showed that release of ALN from ALN-eluting BCP scaffolds was sustained for up to 28 days. In vitro results revealed that MG-63 cells grown on ALN-eluting BCP scaffolds exhibited increased ALP activity and calcium deposition and upregulated gene expression of Runx2, ALP, OCN, and OPN compared with the BCP scaffold alone. Therefore, this study suggests that ALN-eluting BCP scaffolds have the potential to effectively stimulate osteogenic differentiation.

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JF - BioMed Research International

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ER -

Alendronate-eluting biphasic calcium phosphate (BCP) scaffolds stimulate osteogenic differentiation

Abstract

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ASJC Scopus subject areas

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