Altered presynaptic function and number of mitochondria in the medial prefrontal cortex of adult Cyfip2 heterozygous mice

Gyu Hyun Kim, Yinhua Zhang, Hyae Rim Kang, Seung Hyun Lee, Jiwon Shin, Chan Hee Lee, Hyojin Kang, Ruiying Ma, Chunmei Jin, Yoonhee Kim, Su Yeon Kim, Seok Kyu Kwon, Se Young Choi, Kea Joo Lee, Kihoon Han

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7 Citations (Scopus)


Variants of the cytoplasmic FMR1-interacting protein (CYFIP) gene family, CYFIP1 and CYFIP2, are associated with numerous neurodevelopmental and neuropsychiatric disorders. According to several studies, CYFIP1 regulates the development and function of both pre- and post-synapses in neurons. Furthermore, various studies have evaluated CYFIP2 functions in the postsynaptic compartment, such as regulating dendritic spine morphology; however, no study has evaluated whether and how CYFIP2 affects presynaptic functions. To address this issue, in this study, we have focused on the presynapses of layer 5 neurons of the medial prefrontal cortex (mPFC) in adult Cyfip2 heterozygous (Cyfip2 +/- ) mice. Electrophysiological analyses revealed an enhancement in the presynaptic short-term plasticity induced by high-frequency stimuli in Cyfip2 +/- neurons compared with wild-type neurons. Since presynaptic mitochondria play an important role in buffering presynaptic Ca2+, which is directly associated with the short-term plasticity, we analyzed presynaptic mitochondria using electron microscopic images of the mPFC. Compared with wild-type mice, the number, but not the volume or cristae density, of mitochondria in both presynaptic boutons and axonal processes in the mPFC layer 5 of Cyfip2 +/- mice was reduced. Consistent with an identification of mitochondrial proteins in a previously established CYFIP2 interactome, CYFIP2 was detected in a biochemically enriched mitochondrial fraction of the mouse mPFC. Collectively, these results suggest roles for CYFIP2 in regulating presynaptic functions, which may involve presynaptic mitochondrial changes.

Original languageEnglish
Article number123
JournalMolecular brain
Issue number1
Publication statusPublished - 2020 Sept 11

Bibliographical note

Publisher Copyright:
© 2020 The Author(s).


  • Cytoplasmic FMR1-interacting protein 2
  • Medial prefrontal cortex
  • Mitochondria
  • Presynapse

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience


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