Abstract
High expression of osmotically responsive genes1 (HOS1), a key regulator of low temperature response and flowering time, encodes an E3 ubiquitin ligase in Arabidopsis. Here, we report characterization of a newly identified splice variant (HOS1-L) of HOS1. Comparative analyses revealed that HOS1-L has a longer 5' nucleotide sequence than that of the previously identified HOS1 (HOS1-S) and that itsprotein sequence was more conserved than that of HOS1-S in plants. HOS1-L transcripts were spatio-temporally more abundant than those of HOS1-S. The recovery rate of HOS1-S expression was faster than that of HOS1-L after cold treatment. Diurnal oscillation patterns of HOS1-L revealed that HOS1-L expression was affected by photoperiod. An in vitro pull-down assay revealed that the HOS1-L protein interacted with the ICE1 protein. HOS1-L overexpression caused delayed flowering in wild-type plants. Collectively, these results suggest regulation of HOS1 expression at the post-transcriptional level.
Original language | English |
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Pages (from-to) | 515-520 |
Number of pages | 6 |
Journal | BMB reports |
Volume | 45 |
Issue number | 9 |
DOIs | |
Publication status | Published - 2012 |
Keywords
- AAB87130
- Alternative splicing
- BT005517
- HOS1
- Posttranscriptional regulation
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology