Alternative splicing and expression analysis of High expression of osmotically responsive genes1 (HOS1) in Arabidopsis

Jeong Hwan Lee, Soo Hyun Kim, Jae Joon Kim, Ji Hoon Ahn

    Research output: Contribution to journalArticlepeer-review

    11 Citations (Scopus)

    Abstract

    High expression of osmotically responsive genes1 (HOS1), a key regulator of low temperature response and flowering time, encodes an E3 ubiquitin ligase in Arabidopsis. Here, we report characterization of a newly identified splice variant (HOS1-L) of HOS1. Comparative analyses revealed that HOS1-L has a longer 5' nucleotide sequence than that of the previously identified HOS1 (HOS1-S) and that itsprotein sequence was more conserved than that of HOS1-S in plants. HOS1-L transcripts were spatio-temporally more abundant than those of HOS1-S. The recovery rate of HOS1-S expression was faster than that of HOS1-L after cold treatment. Diurnal oscillation patterns of HOS1-L revealed that HOS1-L expression was affected by photoperiod. An in vitro pull-down assay revealed that the HOS1-L protein interacted with the ICE1 protein. HOS1-L overexpression caused delayed flowering in wild-type plants. Collectively, these results suggest regulation of HOS1 expression at the post-transcriptional level.

    Original languageEnglish
    Pages (from-to)515-520
    Number of pages6
    JournalBMB reports
    Volume45
    Issue number9
    DOIs
    Publication statusPublished - 2012

    Keywords

    • AAB87130
    • Alternative splicing
    • BT005517
    • HOS1
    • Posttranscriptional regulation

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology

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