Alternative splicing generates a novel non-secretable cytosolic isoform of NELL2

Eun Mi Hwang, Dong Gyu Kim, Byung Ju Lee, Jungil Choi, Eunju Kim, Nammi Park, Dawon Kang, Jaehee Han, Wan Sung Choi, Seong Geun Hong, Jae Yong Park

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)


NELL2 is as a neuron-specific secreted glycoprotein. The present study provides evidence of an alternatively spliced variant of the rat NELL2 gene that yields cytosolic NELL2 (cNELL2). cNELL2 was initially detected in the thymus and subsequently found to be ubiquitously expressed in many other tissues. The absence of the sequences corresponding to the third exon, which contains the terminal portion of the signal peptide, accounts for the uniform distribution of cNELL2 throughout the cytoplasm. This is in contrast to NELL2, which is preferentially located at distinct subcellular structures involved in the secretary process, such as endoplasmic reticulum and Golgi apparatus. Western blot analysis showed that cNELL2 was not present in the medium but only in lysates, while NELL2 was detected as a glycosylated larger form in both lysates and media. Immunoprecipitation analysis revealed that cNELL2 interacts with PKCβ1. These results suggest that cNELL2 is involved in PKCβ1-mediated intracellular signaling.

Original languageEnglish
Pages (from-to)805-811
Number of pages7
JournalBiochemical and biophysical research communications
Issue number3
Publication statusPublished - 2007 Feb 16
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by a grant from the Basic Research Program of the Korean Science & Engineering Foundation (R13-2005-012-01003-0), and the Korean Research Foundation (KRF-2002-CS0045 and KRF-2006-005-J04204). The nucleotide sequences reported in this paper have been submitted to the GenBank™ databases under Accession No. EF110908 .


  • Alternative splicing
  • Cytosolic NELL2
  • NELL2
  • Signal peptide

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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