Amino acids grafted-chitosan/glycerophosphate hydrogel for controlled release of berberine hydrochloride

Li Jun Ren, Hui Yun Zhou, Pei Yan Hao, Hui Jie Zheng, Jia Nan Tong, Ya Wei Chen, Hyun Jin Park

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


In this paper, l-lysine (Lys), l-glutamate (Glu), and l-glutamine (Gln) grafted-chitosan (CS) polymers (AA-g-CS) are prepared by the one-pot method using EDC/NHS as coupling agents. The introduction of AA into the backbone structure of CS improves its solubility in the pH range of 6.0–7.0. Moreover, AA-g-CS/glycerophosphate hydrogel (AA-g-CS/GP hydrogel) prepared by physical cross-linking has better thermosensitivity and can achieve the sol–gel transition in very short order. AA-g-CS/GP hydrogel has a dense three-dimensional network structure, conducing to the loading of the hydrophobic drugs, and its pore size and pore wall are affected by Lys, Glu, and Gln. Berberine hydrochloride (BBH) is encapsulated within the three-dimensional mesh of the hydrogel. And the addition of BBH has no significant effect on the structure of AA-g-CS/GP hydrogel. The optimal parameters for AA-g-CS/GP hydrogel loaded BBH (BBH@AA-g-CS/GP hydrogel) are the concentration of AA-g-CS is 4.5% and the volume ratio of AA-g-CS/GP is 1.4/0.6. It is shown that BBH@Lys-g-CS/GP hydrogel, BBH@Glu-g-CS/GP hydrogel, and BBH@Gln-g-CS/GP hydrogel have better-sustained release effect. Moreover, the sustained release of BBH@Lys-g-CS/GP hydrogel is the best, and the cumulative release rate is about 56% in 24 h. Therefore, AA-g-CS/GP thermosensitive hydrogel is expected to be an excellent vehicle for sustained release of drugs.

Original languageEnglish
Article numbere53632
JournalJournal of Applied Polymer Science
Issue number11
Publication statusPublished - 2023 Mar 15

Bibliographical note

Funding Information:
The authors are indebted to the financial support from Natural Science Foundation of Henan Province (No. 182300410213) and National Natural Science Foundation of China (No. 51103035).

Funding Information:
National Natural Science Foundation of China, Grant/Award Number: 51103035; Natural Science Foundation of Henan Province, Grant/Award Number: 182300410213 Funding information

Publisher Copyright:
© 2023 Wiley Periodicals LLC.


  • amino acids
  • chitosan
  • glycerophosphate
  • in vitro release
  • thermosensitive hydrogel

ASJC Scopus subject areas

  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Polymers and Plastics
  • Materials Chemistry


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