Amyloid beta detection by faradaic electrochemical impedance spectroscopy using interdigitated microelectrodes

Jin Soo Park; Hye Jin Kim; Ji Hoon Lee; Jung Ho Park; Jinsik Kim; Kyo Seon Hwang; Byung Chul Lee

doi:10.3390/s18020426

Amyloid beta detection by faradaic electrochemical impedance spectroscopy using interdigitated microelectrodes

Jin Soo Park, Hye Jin Kim, Ji Hoon Lee, Jung Ho Park, Jinsik Kim, Kyo Seon Hwang, Byung Chul Lee

School of Electrical Engineering

Research output: Contribution to journal › Article › peer-review

29 Citations (Scopus)

Abstract

Faradaic electrochemical impedance spectroscopy (f-EIS) in the presence of redox reagent, e.g., [Fe(CN)₆]^3-/4-, is widely used in biosensors owing to its high sensitivity. However, in sensors detecting amyloid beta (Aβ), the redox reagent can cause the aggregation of Aβ, which is a disturbance factor in accurate detection. Here, we propose an interdigitated microelectrode (IME) based f-EIS technique that can alleviate the aggregation of Aβ and achieve high sensitivity by buffer control. The proposed method was verified by analyzing three different EIS-based sensors: non-faradaic EIS (nf-EIS), f-EIS, and the proposed f-EIS with buffer control. We analyzed the equivalent circuits of nf-EIS and f-EIS sensors. The dominant factors of sensitivity were analyzed, and the impedance change rates via Aβ reaction was compared. We measured the sensitivity of the IME sensors based on nf-EIS, f-EIS, and the proposed f-EIS. The results demonstrate that the proposed EIS-based IME sensor can detect Aβ with a sensitivity of 7.40-fold and 10.93-fold higher than the nf-EIS and the f-EIS sensors, respectively.

Original language	English
Article number	426
Journal	Sensors (Switzerland)
Volume	18
Issue number	2
DOIs	https://doi.org/10.3390/s18020426
Publication status	Published - 2018 Feb 1

Bibliographical note

Funding Information:
Acknowledgments: This work was supported by the Korea Institute of Science and Technology (KIST) institutional program (2E26840). The authors are grateful for the financial support from the Korea Health Industry Development Institute (KHIDI, HI14C3319). Fabrication of the IME sensors was performed at the KIST Micro-Nano Fabrication center (Seoul, Korea). Scanning electron microscopy (SEM) of the IME sensors was executed at the KIST Advanced Analysis center (Seoul, Korea).

Publisher Copyright:
© 2018 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

Amyloid beta
Biosensor
Faradaic electrochemical impedance spectroscopy
High sensitivity
Redox reagent
[Fe(CN)]

ASJC Scopus subject areas

Analytical Chemistry
Information Systems
Atomic and Molecular Physics, and Optics
Biochemistry
Instrumentation
Electrical and Electronic Engineering

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10.3390/s18020426

Cite this

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title = "Amyloid beta detection by faradaic electrochemical impedance spectroscopy using interdigitated microelectrodes",

abstract = "Faradaic electrochemical impedance spectroscopy (f-EIS) in the presence of redox reagent, e.g., [Fe(CN)6]3-/4-, is widely used in biosensors owing to its high sensitivity. However, in sensors detecting amyloid beta (Aβ), the redox reagent can cause the aggregation of Aβ, which is a disturbance factor in accurate detection. Here, we propose an interdigitated microelectrode (IME) based f-EIS technique that can alleviate the aggregation of Aβ and achieve high sensitivity by buffer control. The proposed method was verified by analyzing three different EIS-based sensors: non-faradaic EIS (nf-EIS), f-EIS, and the proposed f-EIS with buffer control. We analyzed the equivalent circuits of nf-EIS and f-EIS sensors. The dominant factors of sensitivity were analyzed, and the impedance change rates via Aβ reaction was compared. We measured the sensitivity of the IME sensors based on nf-EIS, f-EIS, and the proposed f-EIS. The results demonstrate that the proposed EIS-based IME sensor can detect Aβ with a sensitivity of 7.40-fold and 10.93-fold higher than the nf-EIS and the f-EIS sensors, respectively.",

keywords = "Amyloid beta, Biosensor, Faradaic electrochemical impedance spectroscopy, High sensitivity, Redox reagent, [Fe(CN)]",

author = "Park, {Jin Soo} and Kim, {Hye Jin} and Lee, {Ji Hoon} and Park, {Jung Ho} and Jinsik Kim and Hwang, {Kyo Seon} and Lee, {Byung Chul}",

note = "Funding Information: Acknowledgments: This work was supported by the Korea Institute of Science and Technology (KIST) institutional program (2E26840). The authors are grateful for the financial support from the Korea Health Industry Development Institute (KHIDI, HI14C3319). Fabrication of the IME sensors was performed at the KIST Micro-Nano Fabrication center (Seoul, Korea). Scanning electron microscopy (SEM) of the IME sensors was executed at the KIST Advanced Analysis center (Seoul, Korea). Publisher Copyright: {\textcopyright} 2018 by the authors. Licensee MDPI, Basel, Switzerland.",

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AU - Park, Jung Ho

AU - Kim, Jinsik

AU - Hwang, Kyo Seon

AU - Lee, Byung Chul

N1 - Funding Information: Acknowledgments: This work was supported by the Korea Institute of Science and Technology (KIST) institutional program (2E26840). The authors are grateful for the financial support from the Korea Health Industry Development Institute (KHIDI, HI14C3319). Fabrication of the IME sensors was performed at the KIST Micro-Nano Fabrication center (Seoul, Korea). Scanning electron microscopy (SEM) of the IME sensors was executed at the KIST Advanced Analysis center (Seoul, Korea). Publisher Copyright: © 2018 by the authors. Licensee MDPI, Basel, Switzerland.

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N2 - Faradaic electrochemical impedance spectroscopy (f-EIS) in the presence of redox reagent, e.g., [Fe(CN)6]3-/4-, is widely used in biosensors owing to its high sensitivity. However, in sensors detecting amyloid beta (Aβ), the redox reagent can cause the aggregation of Aβ, which is a disturbance factor in accurate detection. Here, we propose an interdigitated microelectrode (IME) based f-EIS technique that can alleviate the aggregation of Aβ and achieve high sensitivity by buffer control. The proposed method was verified by analyzing three different EIS-based sensors: non-faradaic EIS (nf-EIS), f-EIS, and the proposed f-EIS with buffer control. We analyzed the equivalent circuits of nf-EIS and f-EIS sensors. The dominant factors of sensitivity were analyzed, and the impedance change rates via Aβ reaction was compared. We measured the sensitivity of the IME sensors based on nf-EIS, f-EIS, and the proposed f-EIS. The results demonstrate that the proposed EIS-based IME sensor can detect Aβ with a sensitivity of 7.40-fold and 10.93-fold higher than the nf-EIS and the f-EIS sensors, respectively.

AB - Faradaic electrochemical impedance spectroscopy (f-EIS) in the presence of redox reagent, e.g., [Fe(CN)6]3-/4-, is widely used in biosensors owing to its high sensitivity. However, in sensors detecting amyloid beta (Aβ), the redox reagent can cause the aggregation of Aβ, which is a disturbance factor in accurate detection. Here, we propose an interdigitated microelectrode (IME) based f-EIS technique that can alleviate the aggregation of Aβ and achieve high sensitivity by buffer control. The proposed method was verified by analyzing three different EIS-based sensors: non-faradaic EIS (nf-EIS), f-EIS, and the proposed f-EIS with buffer control. We analyzed the equivalent circuits of nf-EIS and f-EIS sensors. The dominant factors of sensitivity were analyzed, and the impedance change rates via Aβ reaction was compared. We measured the sensitivity of the IME sensors based on nf-EIS, f-EIS, and the proposed f-EIS. The results demonstrate that the proposed EIS-based IME sensor can detect Aβ with a sensitivity of 7.40-fold and 10.93-fold higher than the nf-EIS and the f-EIS sensors, respectively.

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JO - Sensors (Switzerland)

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M1 - 426

ER -

Amyloid beta detection by faradaic electrochemical impedance spectroscopy using interdigitated microelectrodes

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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