Abstract
Peroxynitrite (ONOO−) plays a critical role in Alzheimer's disease (AD). To reveal the ONOO− influx in AD brains, an activatable activity-based fluorescence probe Rd-DPA3 was designed by a structure-modulated strategy. Taking advantage of ONOO−-initiated two-step cascade reactions of a novel chemical trigger, Rd-DPA3 specifically responds to ONOO− in 0.3 mM of other reactive oxygen species (ROS) and varied proteins, and gives an intensive fluorescence enhancement (F/F0=50). Moreover, with its mitochondria-targeting ability, Rd-DPA3 can be used to efficiently monitor the alternations of intracellular ONOO− levels in cerebral cells during oxidative stress. Significantly, due to NIR emission and good blood–brain barrier (BBB) crossing ability, Rd-DPA3 is suitable for in vivo imaging of cerebral ONOO− influx and illustrating an age-dependent accumulation of ONOO− in AD mice brains.
Original language | English |
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Article number | e202206894 |
Journal | Angewandte Chemie - International Edition |
Volume | 61 |
Issue number | 36 |
DOIs | |
Publication status | Published - 2022 Sept 5 |
Keywords
- Activity-Based Sensing
- Alzheimer's Disease
- Fluorescent Probe
- In Vivo Imaging
- Peroxynitrite
ASJC Scopus subject areas
- Catalysis
- Chemistry(all)