An ammonium sulfate sensitive endoxylanase produced by Streptomyces

Jaya Ram Simkhada, Hah Young Yoo, Don Hee Park, Yun Hee Choi, Hyo Jeong Lee, Seung Wook Kim, Jin Cheol Yoo

    Research output: Contribution to journalArticlepeer-review

    4 Citations (Scopus)

    Abstract

    Streptomyces sp. CSWu2 was newly isolated and identified from Korean soil. In culture medium, the strain produced a highly active endoxylanase (Xynwu2), which was purified to homogeneity by a single-step chromatography on Poros-HQ. The xylanase was ∼38 kDa and its activity was maximal at 65 °C and pH 11.0. It was stable up to 60 °C and from pH 8.0 to 12.0, and its activity was slightly enhanced by nonionic detergents, but inhibited by EDTA, EGTA, and divalent metal ions. Intriguingly, Xynwu2 was highly sensitive to ammonium sulfate, but its completely suppressed activity was recovered by desalting out. Xynwu2 produced xylose and xylobiose as principal end products from xylan, suggesting an endoxylanase nature. Importantly, scanning electron microscopy showed Xynwu2 efficiently degraded corncobs, an agro-industrial waste material. We believe that Xynwu2 is a potential candidate for converting lignocellulosic waste material into simple sugars which could be used to produce bioethanol and other value-added products.

    Original languageEnglish
    Pages (from-to)819-825
    Number of pages7
    JournalBioprocess and Biosystems Engineering
    Volume36
    Issue number6
    DOIs
    Publication statusPublished - 2013 Jun

    Bibliographical note

    Funding Information:
    Acknowledgments This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (MEST) (2010-0029178) and a grant from the Next-Generation BioGreen 21 Program (No. PJ008140), Rural Development Administration, Republic of Korea.

    Keywords

    • Agro-industrial biomass
    • Corncob
    • Ion exchange chromatography
    • Streptomyces sp. CSWu2
    • Xylanase

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering

    Fingerprint

    Dive into the research topics of 'An ammonium sulfate sensitive endoxylanase produced by Streptomyces'. Together they form a unique fingerprint.

    Cite this