TY - JOUR
T1 - An ammonium sulfate sensitive endoxylanase produced by Streptomyces
AU - Simkhada, Jaya Ram
AU - Yoo, Hah Young
AU - Park, Don Hee
AU - Choi, Yun Hee
AU - Lee, Hyo Jeong
AU - Kim, Seung Wook
AU - Yoo, Jin Cheol
N1 - Funding Information:
Acknowledgments This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (MEST) (2010-0029178) and a grant from the Next-Generation BioGreen 21 Program (No. PJ008140), Rural Development Administration, Republic of Korea.
PY - 2013/6
Y1 - 2013/6
N2 - Streptomyces sp. CSWu2 was newly isolated and identified from Korean soil. In culture medium, the strain produced a highly active endoxylanase (Xynwu2), which was purified to homogeneity by a single-step chromatography on Poros-HQ. The xylanase was ∼38 kDa and its activity was maximal at 65 °C and pH 11.0. It was stable up to 60 °C and from pH 8.0 to 12.0, and its activity was slightly enhanced by nonionic detergents, but inhibited by EDTA, EGTA, and divalent metal ions. Intriguingly, Xynwu2 was highly sensitive to ammonium sulfate, but its completely suppressed activity was recovered by desalting out. Xynwu2 produced xylose and xylobiose as principal end products from xylan, suggesting an endoxylanase nature. Importantly, scanning electron microscopy showed Xynwu2 efficiently degraded corncobs, an agro-industrial waste material. We believe that Xynwu2 is a potential candidate for converting lignocellulosic waste material into simple sugars which could be used to produce bioethanol and other value-added products.
AB - Streptomyces sp. CSWu2 was newly isolated and identified from Korean soil. In culture medium, the strain produced a highly active endoxylanase (Xynwu2), which was purified to homogeneity by a single-step chromatography on Poros-HQ. The xylanase was ∼38 kDa and its activity was maximal at 65 °C and pH 11.0. It was stable up to 60 °C and from pH 8.0 to 12.0, and its activity was slightly enhanced by nonionic detergents, but inhibited by EDTA, EGTA, and divalent metal ions. Intriguingly, Xynwu2 was highly sensitive to ammonium sulfate, but its completely suppressed activity was recovered by desalting out. Xynwu2 produced xylose and xylobiose as principal end products from xylan, suggesting an endoxylanase nature. Importantly, scanning electron microscopy showed Xynwu2 efficiently degraded corncobs, an agro-industrial waste material. We believe that Xynwu2 is a potential candidate for converting lignocellulosic waste material into simple sugars which could be used to produce bioethanol and other value-added products.
KW - Agro-industrial biomass
KW - Corncob
KW - Ion exchange chromatography
KW - Streptomyces sp. CSWu2
KW - Xylanase
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U2 - 10.1007/s00449-013-0908-4
DO - 10.1007/s00449-013-0908-4
M3 - Article
C2 - 23397448
AN - SCOPUS:84878757154
SN - 1615-7591
VL - 36
SP - 819
EP - 825
JO - Bioprocess and Biosystems Engineering
JF - Bioprocess and Biosystems Engineering
IS - 6
ER -