An ammonium sulfate sensitive endoxylanase produced by Streptomyces

Jaya Ram Simkhada; Hah Young Yoo; Don Hee Park; Yun Hee Choi; Hyo Jeong Lee; Seung Wook Kim; Jin Cheol Yoo

doi:10.1007/s00449-013-0908-4

An ammonium sulfate sensitive endoxylanase produced by Streptomyces

Jaya Ram Simkhada, Hah Young Yoo, Don Hee Park, Yun Hee Choi, Hyo Jeong Lee, Seung Wook Kim, Jin Cheol Yoo

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Streptomyces sp. CSWu2 was newly isolated and identified from Korean soil. In culture medium, the strain produced a highly active endoxylanase (Xynwu2), which was purified to homogeneity by a single-step chromatography on Poros-HQ. The xylanase was ∼38 kDa and its activity was maximal at 65 °C and pH 11.0. It was stable up to 60 °C and from pH 8.0 to 12.0, and its activity was slightly enhanced by nonionic detergents, but inhibited by EDTA, EGTA, and divalent metal ions. Intriguingly, Xynwu2 was highly sensitive to ammonium sulfate, but its completely suppressed activity was recovered by desalting out. Xynwu2 produced xylose and xylobiose as principal end products from xylan, suggesting an endoxylanase nature. Importantly, scanning electron microscopy showed Xynwu2 efficiently degraded corncobs, an agro-industrial waste material. We believe that Xynwu2 is a potential candidate for converting lignocellulosic waste material into simple sugars which could be used to produce bioethanol and other value-added products.

Original language	English
Pages (from-to)	819-825
Number of pages	7
Journal	Bioprocess and Biosystems Engineering
Volume	36
Issue number	6
DOIs	https://doi.org/10.1007/s00449-013-0908-4
Publication status	Published - 2013 Jun

Bibliographical note

Funding Information:
Acknowledgments This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (MEST) (2010-0029178) and a grant from the Next-Generation BioGreen 21 Program (No. PJ008140), Rural Development Administration, Republic of Korea.

Keywords

Agro-industrial biomass
Corncob
Ion exchange chromatography
Streptomyces sp. CSWu2
Xylanase

ASJC Scopus subject areas

Biotechnology
Bioengineering

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10.1007/s00449-013-0908-4

Cite this

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title = "An ammonium sulfate sensitive endoxylanase produced by Streptomyces",

abstract = "Streptomyces sp. CSWu2 was newly isolated and identified from Korean soil. In culture medium, the strain produced a highly active endoxylanase (Xynwu2), which was purified to homogeneity by a single-step chromatography on Poros-HQ. The xylanase was ∼38 kDa and its activity was maximal at 65 °C and pH 11.0. It was stable up to 60 °C and from pH 8.0 to 12.0, and its activity was slightly enhanced by nonionic detergents, but inhibited by EDTA, EGTA, and divalent metal ions. Intriguingly, Xynwu2 was highly sensitive to ammonium sulfate, but its completely suppressed activity was recovered by desalting out. Xynwu2 produced xylose and xylobiose as principal end products from xylan, suggesting an endoxylanase nature. Importantly, scanning electron microscopy showed Xynwu2 efficiently degraded corncobs, an agro-industrial waste material. We believe that Xynwu2 is a potential candidate for converting lignocellulosic waste material into simple sugars which could be used to produce bioethanol and other value-added products.",

keywords = "Agro-industrial biomass, Corncob, Ion exchange chromatography, Streptomyces sp. CSWu2, Xylanase",

author = "Simkhada, {Jaya Ram} and Yoo, {Hah Young} and Park, {Don Hee} and Choi, {Yun Hee} and Lee, {Hyo Jeong} and Kim, {Seung Wook} and Yoo, {Jin Cheol}",

note = "Funding Information: Acknowledgments This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (MEST) (2010-0029178) and a grant from the Next-Generation BioGreen 21 Program (No. PJ008140), Rural Development Administration, Republic of Korea.",

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AU - Simkhada, Jaya Ram

AU - Yoo, Hah Young

AU - Park, Don Hee

AU - Choi, Yun Hee

AU - Lee, Hyo Jeong

AU - Kim, Seung Wook

AU - Yoo, Jin Cheol

N1 - Funding Information: Acknowledgments This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (MEST) (2010-0029178) and a grant from the Next-Generation BioGreen 21 Program (No. PJ008140), Rural Development Administration, Republic of Korea.

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N2 - Streptomyces sp. CSWu2 was newly isolated and identified from Korean soil. In culture medium, the strain produced a highly active endoxylanase (Xynwu2), which was purified to homogeneity by a single-step chromatography on Poros-HQ. The xylanase was ∼38 kDa and its activity was maximal at 65 °C and pH 11.0. It was stable up to 60 °C and from pH 8.0 to 12.0, and its activity was slightly enhanced by nonionic detergents, but inhibited by EDTA, EGTA, and divalent metal ions. Intriguingly, Xynwu2 was highly sensitive to ammonium sulfate, but its completely suppressed activity was recovered by desalting out. Xynwu2 produced xylose and xylobiose as principal end products from xylan, suggesting an endoxylanase nature. Importantly, scanning electron microscopy showed Xynwu2 efficiently degraded corncobs, an agro-industrial waste material. We believe that Xynwu2 is a potential candidate for converting lignocellulosic waste material into simple sugars which could be used to produce bioethanol and other value-added products.

AB - Streptomyces sp. CSWu2 was newly isolated and identified from Korean soil. In culture medium, the strain produced a highly active endoxylanase (Xynwu2), which was purified to homogeneity by a single-step chromatography on Poros-HQ. The xylanase was ∼38 kDa and its activity was maximal at 65 °C and pH 11.0. It was stable up to 60 °C and from pH 8.0 to 12.0, and its activity was slightly enhanced by nonionic detergents, but inhibited by EDTA, EGTA, and divalent metal ions. Intriguingly, Xynwu2 was highly sensitive to ammonium sulfate, but its completely suppressed activity was recovered by desalting out. Xynwu2 produced xylose and xylobiose as principal end products from xylan, suggesting an endoxylanase nature. Importantly, scanning electron microscopy showed Xynwu2 efficiently degraded corncobs, an agro-industrial waste material. We believe that Xynwu2 is a potential candidate for converting lignocellulosic waste material into simple sugars which could be used to produce bioethanol and other value-added products.

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An ammonium sulfate sensitive endoxylanase produced by Streptomyces

Abstract

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Keywords

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