An Arabidopsis NAC transcription factor NAC4 promotes pathogen-induced cell death under negative regulation by microRNA164

Myoung Hoon Lee; Hwi Seong Jeon; Hye Gi Kim; Ohkmae K. Park

doi:10.1111/nph.14371

An Arabidopsis NAC transcription factor NAC4 promotes pathogen-induced cell death under negative regulation by microRNA164

Myoung Hoon Lee, Hwi Seong Jeon, Hye Gi Kim, Ohkmae K. Park^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

116 Citations (Scopus)

Abstract

Hypersensitive response (HR) is a form of programmed cell death (PCD) and the primary immune response that prevents pathogen invasion in plants. Here, we show that a microRNAmiR164 and its target gene NAC4 (At5g07680), encoding a NAC transcription factor, play essential roles in the regulation of HR PCD in Arabidopsis thaliana. Cell death symptoms were noticeably enhanced in NAC4-overexpressing (35S:NAC4) and mir164 mutant plants in response to avirulent bacterial pathogens. NAC4 expression was induced by pathogen infection and negatively regulated by miR164 expression. NAC4-binding DNA sequences were determined by in vitro binding site selection using random oligonucleotide sequences. Microarray, chromatin immunoprecipitation and quantitative real time polymerase chain reaction (qRT-PCR) analyses, followed by cell death assays in protoplasts, led to the identification of NAC4 target genes LURP1, WRKY40 and WRKY54, which act as negative regulators of cell death. Our results suggest that NAC4 promotes hypersensitive cell death by suppressing its target genes and this immune process is fine-tuned by the negative action of miR164.

Original language	English
Pages (from-to)	343-360
Number of pages	18
Journal	New Phytologist
Volume	214
Issue number	1
DOIs	https://doi.org/10.1111/nph.14371
Publication status	Published - 2017 Apr 1

Bibliographical note

Funding Information:
We thank Dr Elliot Meyerowitz for mir164a-4, mir164b-1 and mir164c-2 seeds, Dr Hongwei Guo for mir164abc seeds, Dr Mathilde Fagard for wrky54-2 seeds, and Dr Giltsu Choi for pifQ and phyB seeds. This work was supported by a Korea University grant, the Next-Generation BioGreen 21 Programs (SSAC, PJ01120901; Plant Molecular Breeding Center, PJ01121001) through the Rural Development Administration, and a National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIP) (2013R1A2A2A01067151).

Publisher Copyright:
© 2016 The Authors. New Phytologist © 2016 New Phytologist Trust

Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.

Keywords

Arabidopsis
NAC4
hypersensitive cell death
immunity
microRNA164
transcriptional regulation

ASJC Scopus subject areas

Physiology
Plant Science

Access to Document

10.1111/nph.14371

Cite this

@article{50b1e18ea0af41d6a68fa506a4ceb7da,

title = "An Arabidopsis NAC transcription factor NAC4 promotes pathogen-induced cell death under negative regulation by microRNA164",

abstract = "Hypersensitive response (HR) is a form of programmed cell death (PCD) and the primary immune response that prevents pathogen invasion in plants. Here, we show that a microRNAmiR164 and its target gene NAC4 (At5g07680), encoding a NAC transcription factor, play essential roles in the regulation of HR PCD in Arabidopsis thaliana. Cell death symptoms were noticeably enhanced in NAC4-overexpressing (35S:NAC4) and mir164 mutant plants in response to avirulent bacterial pathogens. NAC4 expression was induced by pathogen infection and negatively regulated by miR164 expression. NAC4-binding DNA sequences were determined by in vitro binding site selection using random oligonucleotide sequences. Microarray, chromatin immunoprecipitation and quantitative real time polymerase chain reaction (qRT-PCR) analyses, followed by cell death assays in protoplasts, led to the identification of NAC4 target genes LURP1, WRKY40 and WRKY54, which act as negative regulators of cell death. Our results suggest that NAC4 promotes hypersensitive cell death by suppressing its target genes and this immune process is fine-tuned by the negative action of miR164.",

keywords = "Arabidopsis, NAC4, hypersensitive cell death, immunity, microRNA164, transcriptional regulation",

author = "Lee, \{Myoung Hoon\} and Jeon, \{Hwi Seong\} and Kim, \{Hye Gi\} and Park, \{Ohkmae K.\}",

note = "Funding Information: We thank Dr Elliot Meyerowitz for mir164a-4, mir164b-1 and mir164c-2 seeds, Dr Hongwei Guo for mir164abc seeds, Dr Mathilde Fagard for wrky54-2 seeds, and Dr Giltsu Choi for pifQ and phyB seeds. This work was supported by a Korea University grant, the Next-Generation BioGreen 21 Programs (SSAC, PJ01120901; Plant Molecular Breeding Center, PJ01121001) through the Rural Development Administration, and a National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIP) (2013R1A2A2A01067151). Publisher Copyright: {\textcopyright} 2016 The Authors. New Phytologist {\textcopyright} 2016 New Phytologist Trust Copyright: Copyright 2017 Elsevier B.V., All rights reserved.",

year = "2017",

month = apr,

day = "1",

doi = "10.1111/nph.14371",

language = "English",

volume = "214",

pages = "343--360",

journal = "New Phytologist",

issn = "0028-646X",

publisher = "Wiley-Blackwell Publishing Ltd",

number = "1",

}

TY - JOUR

T1 - An Arabidopsis NAC transcription factor NAC4 promotes pathogen-induced cell death under negative regulation by microRNA164

AU - Lee, Myoung Hoon

AU - Jeon, Hwi Seong

AU - Kim, Hye Gi

AU - Park, Ohkmae K.

N1 - Funding Information: We thank Dr Elliot Meyerowitz for mir164a-4, mir164b-1 and mir164c-2 seeds, Dr Hongwei Guo for mir164abc seeds, Dr Mathilde Fagard for wrky54-2 seeds, and Dr Giltsu Choi for pifQ and phyB seeds. This work was supported by a Korea University grant, the Next-Generation BioGreen 21 Programs (SSAC, PJ01120901; Plant Molecular Breeding Center, PJ01121001) through the Rural Development Administration, and a National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIP) (2013R1A2A2A01067151). Publisher Copyright: © 2016 The Authors. New Phytologist © 2016 New Phytologist Trust Copyright: Copyright 2017 Elsevier B.V., All rights reserved.

PY - 2017/4/1

Y1 - 2017/4/1

N2 - Hypersensitive response (HR) is a form of programmed cell death (PCD) and the primary immune response that prevents pathogen invasion in plants. Here, we show that a microRNAmiR164 and its target gene NAC4 (At5g07680), encoding a NAC transcription factor, play essential roles in the regulation of HR PCD in Arabidopsis thaliana. Cell death symptoms were noticeably enhanced in NAC4-overexpressing (35S:NAC4) and mir164 mutant plants in response to avirulent bacterial pathogens. NAC4 expression was induced by pathogen infection and negatively regulated by miR164 expression. NAC4-binding DNA sequences were determined by in vitro binding site selection using random oligonucleotide sequences. Microarray, chromatin immunoprecipitation and quantitative real time polymerase chain reaction (qRT-PCR) analyses, followed by cell death assays in protoplasts, led to the identification of NAC4 target genes LURP1, WRKY40 and WRKY54, which act as negative regulators of cell death. Our results suggest that NAC4 promotes hypersensitive cell death by suppressing its target genes and this immune process is fine-tuned by the negative action of miR164.

AB - Hypersensitive response (HR) is a form of programmed cell death (PCD) and the primary immune response that prevents pathogen invasion in plants. Here, we show that a microRNAmiR164 and its target gene NAC4 (At5g07680), encoding a NAC transcription factor, play essential roles in the regulation of HR PCD in Arabidopsis thaliana. Cell death symptoms were noticeably enhanced in NAC4-overexpressing (35S:NAC4) and mir164 mutant plants in response to avirulent bacterial pathogens. NAC4 expression was induced by pathogen infection and negatively regulated by miR164 expression. NAC4-binding DNA sequences were determined by in vitro binding site selection using random oligonucleotide sequences. Microarray, chromatin immunoprecipitation and quantitative real time polymerase chain reaction (qRT-PCR) analyses, followed by cell death assays in protoplasts, led to the identification of NAC4 target genes LURP1, WRKY40 and WRKY54, which act as negative regulators of cell death. Our results suggest that NAC4 promotes hypersensitive cell death by suppressing its target genes and this immune process is fine-tuned by the negative action of miR164.

KW - Arabidopsis

KW - NAC4

KW - hypersensitive cell death

KW - immunity

KW - microRNA164

KW - transcriptional regulation

UR - https://www.scopus.com/pages/publications/85007417849

UR - https://www.scopus.com/inward/citedby.url?scp=85007417849&partnerID=8YFLogxK

U2 - 10.1111/nph.14371

DO - 10.1111/nph.14371

M3 - Article

C2 - 28032643

AN - SCOPUS:85007417849

SN - 0028-646X

VL - 214

SP - 343

EP - 360

JO - New Phytologist

JF - New Phytologist

IS - 1

ER -

An Arabidopsis NAC transcription factor NAC4 promotes pathogen-induced cell death under negative regulation by microRNA164

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this