An Arabidopsis R2R3-MYB transcription factor, AtMYB20, negatively regulates type 2C serine/threonine protein phosphatases to enhance salt tolerance

Mei Hua Cui; Kyoung Shin Yoo; Sujin Hyoung; Ha Thi Kim Nguyen; Yun Young Kim; Hae Jin Kim; Sung Han Ok; Sang Dong Yoo; Jeong Sheop Shin

doi:10.1016/j.febslet.2013.04.028

An Arabidopsis R2R3-MYB transcription factor, AtMYB20, negatively regulates type 2C serine/threonine protein phosphatases to enhance salt tolerance

Mei Hua Cui, Kyoung Shin Yoo, Sujin Hyoung, Ha Thi Kim Nguyen, Yun Young Kim, Hae Jin Kim, Sung Han Ok, Sang Dong Yoo, Jeong Sheop Shin

Division of life Sciences

Research output: Contribution to journal › Article › peer-review

163 Citations (Scopus)

Abstract

We have characterized the function of a plant R2R3-MYB transcription factor, Arabidopsis thaliana MYB20 (AtMYB20). Transgenic plants overexpressing AtMYB20 (AtMYB20-OX) enhanced salt stress tolerance while repression lines (AtMYB20-SRDX) were more vulnerable to NaCl than wild-type plants. Following NaCl treatment, the expressions of ABI1, ABI2 and AtPP2CA, which encode type 2C serine/threonine protein phosphatases (PP2Cs) that act as negative regulators in abscisic acid (ABA) signaling, were suppressed in AtMYB20-OX but induced in AtMYB20-SRDX. The electrophoretic mobility shift assay results revealed that AtMYB20 binds to the promoter regions containing the MYB recognition sequence (TAACTG) and an ACGT core element of ABI1 and AtPP2CA. These findings suggest that AtMYB20 down-regulates the expression of PP2Cs, the negative regulator of ABA signaling, and enhances salt tolerance.

Original language	English
Pages (from-to)	1773-1778
Number of pages	6
Journal	FEBS Letters
Volume	587
Issue number	12
DOIs	https://doi.org/10.1016/j.febslet.2013.04.028
Publication status	Published - 2013 Jun 19

Keywords

Abscisic acid
MYB family
Salt stress
Type 2C serine/threonine protein phosphatase

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2013.04.028

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@article{f46e8439234643129d7ee2f854eb95ff,

title = "An Arabidopsis R2R3-MYB transcription factor, AtMYB20, negatively regulates type 2C serine/threonine protein phosphatases to enhance salt tolerance",

abstract = "We have characterized the function of a plant R2R3-MYB transcription factor, Arabidopsis thaliana MYB20 (AtMYB20). Transgenic plants overexpressing AtMYB20 (AtMYB20-OX) enhanced salt stress tolerance while repression lines (AtMYB20-SRDX) were more vulnerable to NaCl than wild-type plants. Following NaCl treatment, the expressions of ABI1, ABI2 and AtPP2CA, which encode type 2C serine/threonine protein phosphatases (PP2Cs) that act as negative regulators in abscisic acid (ABA) signaling, were suppressed in AtMYB20-OX but induced in AtMYB20-SRDX. The electrophoretic mobility shift assay results revealed that AtMYB20 binds to the promoter regions containing the MYB recognition sequence (TAACTG) and an ACGT core element of ABI1 and AtPP2CA. These findings suggest that AtMYB20 down-regulates the expression of PP2Cs, the negative regulator of ABA signaling, and enhances salt tolerance.",

keywords = "Abscisic acid, MYB family, Salt stress, Type 2C serine/threonine protein phosphatase",

author = "Cui, {Mei Hua} and Yoo, {Kyoung Shin} and Sujin Hyoung and Nguyen, {Ha Thi Kim} and Kim, {Yun Young} and Kim, {Hae Jin} and Ok, {Sung Han} and Yoo, {Sang Dong} and Shin, {Jeong Sheop}",

note = "Funding Information: This work was supported by a Grant (2011-0015848) from the National Research Foundation of Korea (NRF) funded by the Ministry of Science and Technology , Grants (PJ008198 and PJ008160) from the Next-Generation BioGreen 21 Program funded by the Rural Development Administration , and a Grant from the Technology Development Program for Agriculture and Forestry funded by the Ministry for Food, Agriculture, Forestry and Fisheries , Republic of Korea. We also thank the Plant Stress Center of Excellence (PSCE), University of Isfahan, Iran, for collaboration. ",

year = "2013",

month = jun,

day = "19",

doi = "10.1016/j.febslet.2013.04.028",

language = "English",

volume = "587",

pages = "1773--1778",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "12",

}

TY - JOUR

T1 - An Arabidopsis R2R3-MYB transcription factor, AtMYB20, negatively regulates type 2C serine/threonine protein phosphatases to enhance salt tolerance

AU - Cui, Mei Hua

AU - Yoo, Kyoung Shin

AU - Hyoung, Sujin

AU - Nguyen, Ha Thi Kim

AU - Kim, Yun Young

AU - Kim, Hae Jin

AU - Ok, Sung Han

AU - Yoo, Sang Dong

AU - Shin, Jeong Sheop

N1 - Funding Information: This work was supported by a Grant (2011-0015848) from the National Research Foundation of Korea (NRF) funded by the Ministry of Science and Technology , Grants (PJ008198 and PJ008160) from the Next-Generation BioGreen 21 Program funded by the Rural Development Administration , and a Grant from the Technology Development Program for Agriculture and Forestry funded by the Ministry for Food, Agriculture, Forestry and Fisheries , Republic of Korea. We also thank the Plant Stress Center of Excellence (PSCE), University of Isfahan, Iran, for collaboration.

PY - 2013/6/19

Y1 - 2013/6/19

N2 - We have characterized the function of a plant R2R3-MYB transcription factor, Arabidopsis thaliana MYB20 (AtMYB20). Transgenic plants overexpressing AtMYB20 (AtMYB20-OX) enhanced salt stress tolerance while repression lines (AtMYB20-SRDX) were more vulnerable to NaCl than wild-type plants. Following NaCl treatment, the expressions of ABI1, ABI2 and AtPP2CA, which encode type 2C serine/threonine protein phosphatases (PP2Cs) that act as negative regulators in abscisic acid (ABA) signaling, were suppressed in AtMYB20-OX but induced in AtMYB20-SRDX. The electrophoretic mobility shift assay results revealed that AtMYB20 binds to the promoter regions containing the MYB recognition sequence (TAACTG) and an ACGT core element of ABI1 and AtPP2CA. These findings suggest that AtMYB20 down-regulates the expression of PP2Cs, the negative regulator of ABA signaling, and enhances salt tolerance.

AB - We have characterized the function of a plant R2R3-MYB transcription factor, Arabidopsis thaliana MYB20 (AtMYB20). Transgenic plants overexpressing AtMYB20 (AtMYB20-OX) enhanced salt stress tolerance while repression lines (AtMYB20-SRDX) were more vulnerable to NaCl than wild-type plants. Following NaCl treatment, the expressions of ABI1, ABI2 and AtPP2CA, which encode type 2C serine/threonine protein phosphatases (PP2Cs) that act as negative regulators in abscisic acid (ABA) signaling, were suppressed in AtMYB20-OX but induced in AtMYB20-SRDX. The electrophoretic mobility shift assay results revealed that AtMYB20 binds to the promoter regions containing the MYB recognition sequence (TAACTG) and an ACGT core element of ABI1 and AtPP2CA. These findings suggest that AtMYB20 down-regulates the expression of PP2Cs, the negative regulator of ABA signaling, and enhances salt tolerance.

KW - Abscisic acid

KW - MYB family

KW - Salt stress

KW - Type 2C serine/threonine protein phosphatase

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U2 - 10.1016/j.febslet.2013.04.028

DO - 10.1016/j.febslet.2013.04.028

M3 - Article

C2 - 23660402

AN - SCOPUS:84878794296

SN - 0014-5793

VL - 587

SP - 1773

EP - 1778

JO - FEBS Letters

JF - FEBS Letters

IS - 12

ER -

An Arabidopsis R2R3-MYB transcription factor, AtMYB20, negatively regulates type 2C serine/threonine protein phosphatases to enhance salt tolerance

Abstract

Keywords

ASJC Scopus subject areas

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