An artificial synthetic pathway for acetoin, 2,3-butanediol, and 2-butanol production from ethanol using cell free multi-enzyme catalysis

Liaoyuan Zhang; Raushan Singh; D. Sivakumar; Zewang Guo; Jiahuan Li; Fanbing Chen; Yuanzhi He; Xiong Guan; Yun Chan Kang; Jung Kul Lee

doi:10.1039/c7gc02898a

An artificial synthetic pathway for acetoin, 2,3-butanediol, and 2-butanol production from ethanol using cell free multi-enzyme catalysis

Liaoyuan Zhang, Raushan Singh, D. Sivakumar, Zewang Guo, Jiahuan Li, Fanbing Chen, Yuanzhi He, Xiong Guan, Yun Chan Kang, Jung Kul Lee

Research output: Contribution to journal › Article › peer-review

72 Citations (Scopus)

Abstract

Upgrading ethanol to higher order alcohols is desired but difficult using current biotechnological methods. In this study, we designed a completely artificial reaction pathway for upgrading ethanol to acetoin, 2,3-butanediol, and 2-butanol in a cell-free bio-system composed of ethanol dehydrogenase, formolase, 2,3-butanediol dehydrogenase, diol dehydratase, and NADH oxidase. Under optimized conditions, acetoin, 2,3-butanediol, and 2-butanol were produced at 88.78%, 88.28%, and 27.25% of the theoretical yield from 100 mM ethanol, respectively. These results demonstrate that this artificial synthetic pathway is an environmentally-friendly novel approach for upgrading bio-ethanol to acetoin, 2,3-butanediol, and 2-butanol.

Original language	English
Pages (from-to)	230-242
Number of pages	13
Journal	Green Chemistry
Volume	20
Issue number	1
DOIs	https://doi.org/10.1039/c7gc02898a
Publication status	Published - 2018

Bibliographical note

Funding Information:
This work was supported by the National Natural Science Foundation of China (no. 81673542), the New Century Excellent Talents Supporting Plan of the Provincial Education Department of Fujian Province of China (no. K8015056A), the Development Platform of Edible Fungi Industry in Fujian Province (no. K5114001A), and the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (2017R1A2B3011676, 2017R1A4A1014806, 2013 M3A6A8073184).

Publisher Copyright:
© 2018 The Royal Society of Chemistry.

ASJC Scopus subject areas

Environmental Chemistry
Pollution

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title = "An artificial synthetic pathway for acetoin, 2,3-butanediol, and 2-butanol production from ethanol using cell free multi-enzyme catalysis",

abstract = "Upgrading ethanol to higher order alcohols is desired but difficult using current biotechnological methods. In this study, we designed a completely artificial reaction pathway for upgrading ethanol to acetoin, 2,3-butanediol, and 2-butanol in a cell-free bio-system composed of ethanol dehydrogenase, formolase, 2,3-butanediol dehydrogenase, diol dehydratase, and NADH oxidase. Under optimized conditions, acetoin, 2,3-butanediol, and 2-butanol were produced at 88.78%, 88.28%, and 27.25% of the theoretical yield from 100 mM ethanol, respectively. These results demonstrate that this artificial synthetic pathway is an environmentally-friendly novel approach for upgrading bio-ethanol to acetoin, 2,3-butanediol, and 2-butanol.",

author = "Liaoyuan Zhang and Raushan Singh and D. Sivakumar and Zewang Guo and Jiahuan Li and Fanbing Chen and Yuanzhi He and Xiong Guan and Kang, {Yun Chan} and Lee, {Jung Kul}",

note = "Funding Information: This work was supported by the National Natural Science Foundation of China (no. 81673542), the New Century Excellent Talents Supporting Plan of the Provincial Education Department of Fujian Province of China (no. K8015056A), the Development Platform of Edible Fungi Industry in Fujian Province (no. K5114001A), and the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (2017R1A2B3011676, 2017R1A4A1014806, 2013 M3A6A8073184). Publisher Copyright: {\textcopyright} 2018 The Royal Society of Chemistry.",

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AU - Zhang, Liaoyuan

AU - Singh, Raushan

AU - Sivakumar, D.

AU - Guo, Zewang

AU - Li, Jiahuan

AU - Chen, Fanbing

AU - He, Yuanzhi

AU - Guan, Xiong

AU - Kang, Yun Chan

AU - Lee, Jung Kul

N1 - Funding Information: This work was supported by the National Natural Science Foundation of China (no. 81673542), the New Century Excellent Talents Supporting Plan of the Provincial Education Department of Fujian Province of China (no. K8015056A), the Development Platform of Edible Fungi Industry in Fujian Province (no. K5114001A), and the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (2017R1A2B3011676, 2017R1A4A1014806, 2013 M3A6A8073184). Publisher Copyright: © 2018 The Royal Society of Chemistry.

PY - 2018

Y1 - 2018

N2 - Upgrading ethanol to higher order alcohols is desired but difficult using current biotechnological methods. In this study, we designed a completely artificial reaction pathway for upgrading ethanol to acetoin, 2,3-butanediol, and 2-butanol in a cell-free bio-system composed of ethanol dehydrogenase, formolase, 2,3-butanediol dehydrogenase, diol dehydratase, and NADH oxidase. Under optimized conditions, acetoin, 2,3-butanediol, and 2-butanol were produced at 88.78%, 88.28%, and 27.25% of the theoretical yield from 100 mM ethanol, respectively. These results demonstrate that this artificial synthetic pathway is an environmentally-friendly novel approach for upgrading bio-ethanol to acetoin, 2,3-butanediol, and 2-butanol.

AB - Upgrading ethanol to higher order alcohols is desired but difficult using current biotechnological methods. In this study, we designed a completely artificial reaction pathway for upgrading ethanol to acetoin, 2,3-butanediol, and 2-butanol in a cell-free bio-system composed of ethanol dehydrogenase, formolase, 2,3-butanediol dehydrogenase, diol dehydratase, and NADH oxidase. Under optimized conditions, acetoin, 2,3-butanediol, and 2-butanol were produced at 88.78%, 88.28%, and 27.25% of the theoretical yield from 100 mM ethanol, respectively. These results demonstrate that this artificial synthetic pathway is an environmentally-friendly novel approach for upgrading bio-ethanol to acetoin, 2,3-butanediol, and 2-butanol.

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An artificial synthetic pathway for acetoin, 2,3-butanediol, and 2-butanol production from ethanol using cell free multi-enzyme catalysis

Abstract

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