TY - JOUR
T1 - An artificial synthetic pathway for acetoin, 2,3-butanediol, and 2-butanol production from ethanol using cell free multi-enzyme catalysis
AU - Zhang, Liaoyuan
AU - Singh, Raushan
AU - Sivakumar, D.
AU - Guo, Zewang
AU - Li, Jiahuan
AU - Chen, Fanbing
AU - He, Yuanzhi
AU - Guan, Xiong
AU - Kang, Yun Chan
AU - Lee, Jung Kul
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of China (no. 81673542), the New Century Excellent Talents Supporting Plan of the Provincial Education Department of Fujian Province of China (no. K8015056A), the Development Platform of Edible Fungi Industry in Fujian Province (no. K5114001A), and the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (2017R1A2B3011676, 2017R1A4A1014806, 2013 M3A6A8073184).
Publisher Copyright:
© 2018 The Royal Society of Chemistry.
PY - 2018
Y1 - 2018
N2 - Upgrading ethanol to higher order alcohols is desired but difficult using current biotechnological methods. In this study, we designed a completely artificial reaction pathway for upgrading ethanol to acetoin, 2,3-butanediol, and 2-butanol in a cell-free bio-system composed of ethanol dehydrogenase, formolase, 2,3-butanediol dehydrogenase, diol dehydratase, and NADH oxidase. Under optimized conditions, acetoin, 2,3-butanediol, and 2-butanol were produced at 88.78%, 88.28%, and 27.25% of the theoretical yield from 100 mM ethanol, respectively. These results demonstrate that this artificial synthetic pathway is an environmentally-friendly novel approach for upgrading bio-ethanol to acetoin, 2,3-butanediol, and 2-butanol.
AB - Upgrading ethanol to higher order alcohols is desired but difficult using current biotechnological methods. In this study, we designed a completely artificial reaction pathway for upgrading ethanol to acetoin, 2,3-butanediol, and 2-butanol in a cell-free bio-system composed of ethanol dehydrogenase, formolase, 2,3-butanediol dehydrogenase, diol dehydratase, and NADH oxidase. Under optimized conditions, acetoin, 2,3-butanediol, and 2-butanol were produced at 88.78%, 88.28%, and 27.25% of the theoretical yield from 100 mM ethanol, respectively. These results demonstrate that this artificial synthetic pathway is an environmentally-friendly novel approach for upgrading bio-ethanol to acetoin, 2,3-butanediol, and 2-butanol.
UR - http://www.scopus.com/inward/record.url?scp=85040191293&partnerID=8YFLogxK
U2 - 10.1039/c7gc02898a
DO - 10.1039/c7gc02898a
M3 - Article
AN - SCOPUS:85040191293
SN - 1463-9262
VL - 20
SP - 230
EP - 242
JO - Green Chemistry
JF - Green Chemistry
IS - 1
ER -