An expansin from the marine bacterium Hahella chejuensis acts synergistically with xylanase and enhances xylan hydrolysis

Hee Jin Lee; In Jung Kim; Jihyun F. Kim; In Geol Choi; Kyoung Heon Kim

doi:10.1016/j.biortech.2013.09.086

An expansin from the marine bacterium Hahella chejuensis acts synergistically with xylanase and enhances xylan hydrolysis

Hee Jin Lee, In Jung Kim, Jihyun F. Kim, In Geol Choi, Kyoung Heon Kim

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

HcEXLX2 is a bacterial expansin found in a marine bacterium, Hahella chejuensis. Previously, HcEXLX2 was reported to act synergistically with a commercial cellulase preparation on the cellulose hydrolysis. The aim of the present study was to investigate the possible synergistic activity of HcEXLX2 with an endo-type xylanase from Saccharophagus degradans 2-40^T (Xyn10C) in the hydrolysis of xylan. When 160μg of HcEXLX2 was incubated with 12μg of Xyn10C, the yield of reducing sugar increased 3.1 times when compared to that without HcEXLX2. The optimal temperature and pH for the synergism of HcEXLX2 with Xyn10C were 30°C and pH 7, respectively. In addition, binding experiments revealed that HcEXLX2 binds to xylan more preferentially than to Avicel. These results imply that HcEXLX2 could be used as an accessory protein to boost the activity of xylanase if its synergistic effect is strengthened at lower dosages.

Original language	English
Pages (from-to)	516
Number of pages	1
Journal	Bioresource technology
Volume	149
DOIs	https://doi.org/10.1016/j.biortech.2013.09.086
Publication status	Published - 2013 Dec

Bibliographical note

Funding Information:
This work was supported by a Grant from the Institute of Life Science and Natural Resources , Korea University. Experiments were carried out using the facilities of the Institute of Biomedical Science and Food Safety at the Food Safety Hall, Korea University.

Keywords

Bacterial expansin
Hahella chejuensis
Synergism
Xylan hydrolysis

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2013.09.086

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title = "An expansin from the marine bacterium Hahella chejuensis acts synergistically with xylanase and enhances xylan hydrolysis",

abstract = "HcEXLX2 is a bacterial expansin found in a marine bacterium, Hahella chejuensis. Previously, HcEXLX2 was reported to act synergistically with a commercial cellulase preparation on the cellulose hydrolysis. The aim of the present study was to investigate the possible synergistic activity of HcEXLX2 with an endo-type xylanase from Saccharophagus degradans 2-40T (Xyn10C) in the hydrolysis of xylan. When 160μg of HcEXLX2 was incubated with 12μg of Xyn10C, the yield of reducing sugar increased 3.1 times when compared to that without HcEXLX2. The optimal temperature and pH for the synergism of HcEXLX2 with Xyn10C were 30°C and pH 7, respectively. In addition, binding experiments revealed that HcEXLX2 binds to xylan more preferentially than to Avicel. These results imply that HcEXLX2 could be used as an accessory protein to boost the activity of xylanase if its synergistic effect is strengthened at lower dosages.",

keywords = "Bacterial expansin, Hahella chejuensis, Synergism, Xylan hydrolysis",

author = "Lee, {Hee Jin} and Kim, {In Jung} and Kim, {Jihyun F.} and Choi, {In Geol} and Kim, {Kyoung Heon}",

note = "Funding Information: This work was supported by a Grant from the Institute of Life Science and Natural Resources , Korea University. Experiments were carried out using the facilities of the Institute of Biomedical Science and Food Safety at the Food Safety Hall, Korea University.",

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doi = "10.1016/j.biortech.2013.09.086",

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AU - Lee, Hee Jin

AU - Kim, In Jung

AU - Kim, Jihyun F.

AU - Choi, In Geol

AU - Kim, Kyoung Heon

N1 - Funding Information: This work was supported by a Grant from the Institute of Life Science and Natural Resources , Korea University. Experiments were carried out using the facilities of the Institute of Biomedical Science and Food Safety at the Food Safety Hall, Korea University.

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Y1 - 2013/12

N2 - HcEXLX2 is a bacterial expansin found in a marine bacterium, Hahella chejuensis. Previously, HcEXLX2 was reported to act synergistically with a commercial cellulase preparation on the cellulose hydrolysis. The aim of the present study was to investigate the possible synergistic activity of HcEXLX2 with an endo-type xylanase from Saccharophagus degradans 2-40T (Xyn10C) in the hydrolysis of xylan. When 160μg of HcEXLX2 was incubated with 12μg of Xyn10C, the yield of reducing sugar increased 3.1 times when compared to that without HcEXLX2. The optimal temperature and pH for the synergism of HcEXLX2 with Xyn10C were 30°C and pH 7, respectively. In addition, binding experiments revealed that HcEXLX2 binds to xylan more preferentially than to Avicel. These results imply that HcEXLX2 could be used as an accessory protein to boost the activity of xylanase if its synergistic effect is strengthened at lower dosages.

AB - HcEXLX2 is a bacterial expansin found in a marine bacterium, Hahella chejuensis. Previously, HcEXLX2 was reported to act synergistically with a commercial cellulase preparation on the cellulose hydrolysis. The aim of the present study was to investigate the possible synergistic activity of HcEXLX2 with an endo-type xylanase from Saccharophagus degradans 2-40T (Xyn10C) in the hydrolysis of xylan. When 160μg of HcEXLX2 was incubated with 12μg of Xyn10C, the yield of reducing sugar increased 3.1 times when compared to that without HcEXLX2. The optimal temperature and pH for the synergism of HcEXLX2 with Xyn10C were 30°C and pH 7, respectively. In addition, binding experiments revealed that HcEXLX2 binds to xylan more preferentially than to Avicel. These results imply that HcEXLX2 could be used as an accessory protein to boost the activity of xylanase if its synergistic effect is strengthened at lower dosages.

KW - Bacterial expansin

KW - Hahella chejuensis

KW - Synergism

KW - Xylan hydrolysis

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An expansin from the marine bacterium Hahella chejuensis acts synergistically with xylanase and enhances xylan hydrolysis

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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