An integrative process for obtaining lipids and glucose from Chlorella vulgaris biomass with a single treatment of cell disruption

Young Mok Heo, Hanbyul Lee, Changsu Lee, Juwon Kang, Joon Woo Ahn, Young Min Lee, Kyu Young Kang, Yoon E. Choi, Jae Jin Kim

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)


To examine the possibility of better utilizing Chlorella vulgaris biomass including its carbohydrate as well as lipid contents, it was investigated whether cell disruption for lipid extraction could render the remaining microalgal residue (MR) suitable for enzymatic saccharification, possibly due to the disruption of cell wall structures. The C. vulgaris biomass was subjected to lipid extraction with different cell disruption methods (autoclaving, microwave irradiation, osmotic shock, and sonication), and recovered MRs were hydrolyzed using an enzyme produced from Trichoderma koningiopsis KUC21269 in this study. The enzyme was produced on-site with a highly simplified medium of barley straw, an agricultural byproduct. As a result, the saccharification rate of MR treated with microwave was more than twice that of the control group, and microwave irradiation appeared to be a promising method for both lipid extraction and subsequent saccharification. Our results suggested that both lipids and carbohydrates in C. vulgaris can be utilized by applying proper cell disruption method and a fungal enzyme produced on-site using an agricultural byproduct, respectively. This study revealed the high potential of C. vulgaris as an integrated bio-resource for both lipids and glucose, which can be converted to biodiesel and bioethanol, providing clues for overcoming hurdles in economically feasible biofuel production using microalgae.

Original languageEnglish
Pages (from-to)286-294
Number of pages9
JournalAlgal Research
Publication statusPublished - 2017 Nov

Bibliographical note

Funding Information:
This research was supported by the Korea Basic Science Institute under the R&D program (Project No. C36703 ) supervised by the Ministry of Science, ICT and Future Planning. This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government ( MSIP ) ( 2016R1D1A1B03932773 ). This research was also supported by the project on survey and excavation of Korean indigenous species of National Institute of Biological Resources ( NIBR 201701104 ) under the Ministry of Environment , Republic of Korea. This research was also supported by a grant from the Marine Biotechnology Program ( 20170488 ) funded by the Ministry of Oceans and Fisheries, Korea.

Publisher Copyright:
© 2017 Elsevier B.V.


  • Bioaccessibility
  • Cell disruption
  • Enzymatic saccharification
  • Lipid-extracted residue
  • Microwave irradiation

ASJC Scopus subject areas

  • Agronomy and Crop Science


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