An ion channel essential for sensing chemical damage

Lindsey J. Macpherson, Bailong Xiao, Kelvin Y. Kwan, Matt J. Petrus, Adrienne E. Dubin, Sun Wook Hwang, Benjamin Cravatt, David P. Corey, Ardem Patapoutian

    Research output: Contribution to journalArticlepeer-review

    247 Citations (Scopus)

    Abstract

    Tissue damage and its downstream consequences are experimentally assayed by formaldehyde application, which indiscriminately modifies proteins and is presumed to cause pain through broadly acting mechanisms. Here we show that formaldehyde activates the ion channel TRPA1 and that TRPA1-deficient mice exhibit dramatically reduced formaldehyde-induced pain responses. 4-Hydroxynonenal, a reactive chemical produced endogenously during oxidative stress, and other related aldehydes also activate TRPA1 in vitro. Furthermore, painful responses to iodoacetamide, a nonspecific cysteine-alkylating compound, are abolished in TRPA1-deficient mice. Therefore, although these reactive chemicals modify many proteins, the associated pain appears mainly dependent on a single ion channel.

    Original languageEnglish
    Pages (from-to)11412-11415
    Number of pages4
    JournalJournal of Neuroscience
    Volume27
    Issue number42
    DOIs
    Publication statusPublished - 2007 Oct 17

    Keywords

    • 4-HNE
    • Formaldehyde
    • Pain
    • Somatosensory
    • TRP
    • TRPA1
    • ThermoTRP

    ASJC Scopus subject areas

    • General Neuroscience

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