In this study, we successfully developed a ssDNA aptamer pairs by using an advanced immobilization-free SELEX method with affinity-based selection and counter-screening process at every round. By implementing this method, two different aptamers specifically binding to bovine viral diarrhea virus type 1(BVDV type 1) with high affinity were successfully screened. This aptamer pair was applied to ultrasensitive detection platform for BVDV type 1 in a sandwich manner. The ultrasensitive detection of BVDV type 1 using one of aptamers conjugated with gold nanoparticles was obtained in aptamer-aptamer sandwich type sensing format, with the limit of detection of 800. copies/ml, which is comparable to a real-time PCR method.
Bibliographical noteFunding Information:
This work was supported by the Advanced Production Technology Development Program for Agriculture and Forestry in the Ministry for Food , the Agriculture, Forestry and Fisheries, Republic of Korea and the National Research Foundation of Korea (NRF) Grant funded by the Korea Government (MEST) (No. 2012R1A2A2A01011056 ).
- Bovine viral diarrhea virus (BVDV)
- Dual aptamers
ASJC Scopus subject areas
- Biomedical Engineering