An ultra-sensitive detection of a whole virus using dual aptamers developed by immobilization-free screening

Jee Woong Park; Su Jin Lee; Eun Jin Choi; Jaejo Kim; Jae Young Song; Man Bock Gu

doi:10.1016/j.bios.2013.07.052

An ultra-sensitive detection of a whole virus using dual aptamers developed by immobilization-free screening

Jee Woong Park, Su Jin Lee, Eun Jin Choi, Jaejo Kim, Jae Young Song, Man Bock Gu

Research output: Contribution to journal › Article › peer-review

74 Citations (Scopus)

Abstract

In this study, we successfully developed a ssDNA aptamer pairs by using an advanced immobilization-free SELEX method with affinity-based selection and counter-screening process at every round. By implementing this method, two different aptamers specifically binding to bovine viral diarrhea virus type 1(BVDV type 1) with high affinity were successfully screened. This aptamer pair was applied to ultrasensitive detection platform for BVDV type 1 in a sandwich manner. The ultrasensitive detection of BVDV type 1 using one of aptamers conjugated with gold nanoparticles was obtained in aptamer-aptamer sandwich type sensing format, with the limit of detection of 800. copies/ml, which is comparable to a real-time PCR method.

Original language	English
Pages (from-to)	324-329
Number of pages	6
Journal	Biosensors and Bioelectronics
Volume	51
DOIs	https://doi.org/10.1016/j.bios.2013.07.052
Publication status	Published - 2014 Jan 5

Bibliographical note

Funding Information:
This work was supported by the Advanced Production Technology Development Program for Agriculture and Forestry in the Ministry for Food , the Agriculture, Forestry and Fisheries, Republic of Korea and the National Research Foundation of Korea (NRF) Grant funded by the Korea Government (MEST) (No. 2012R1A2A2A01011056 ).

Keywords

Bovine viral diarrhea virus (BVDV)
Dual aptamers
SELEX
SPR
Sandwich

ASJC Scopus subject areas

Biotechnology
Biophysics
Biomedical Engineering
Electrochemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bios.2013.07.052

Cite this

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abstract = "In this study, we successfully developed a ssDNA aptamer pairs by using an advanced immobilization-free SELEX method with affinity-based selection and counter-screening process at every round. By implementing this method, two different aptamers specifically binding to bovine viral diarrhea virus type 1(BVDV type 1) with high affinity were successfully screened. This aptamer pair was applied to ultrasensitive detection platform for BVDV type 1 in a sandwich manner. The ultrasensitive detection of BVDV type 1 using one of aptamers conjugated with gold nanoparticles was obtained in aptamer-aptamer sandwich type sensing format, with the limit of detection of 800. copies/ml, which is comparable to a real-time PCR method.",

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author = "Park, {Jee Woong} and {Jin Lee}, Su and Choi, {Eun Jin} and Jaejo Kim and Song, {Jae Young} and {Bock Gu}, Man",

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AU - Park, Jee Woong

AU - Jin Lee, Su

AU - Choi, Eun Jin

AU - Kim, Jaejo

AU - Song, Jae Young

AU - Bock Gu, Man

N1 - Funding Information: This work was supported by the Advanced Production Technology Development Program for Agriculture and Forestry in the Ministry for Food , the Agriculture, Forestry and Fisheries, Republic of Korea and the National Research Foundation of Korea (NRF) Grant funded by the Korea Government (MEST) (No. 2012R1A2A2A01011056 ).

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AB - In this study, we successfully developed a ssDNA aptamer pairs by using an advanced immobilization-free SELEX method with affinity-based selection and counter-screening process at every round. By implementing this method, two different aptamers specifically binding to bovine viral diarrhea virus type 1(BVDV type 1) with high affinity were successfully screened. This aptamer pair was applied to ultrasensitive detection platform for BVDV type 1 in a sandwich manner. The ultrasensitive detection of BVDV type 1 using one of aptamers conjugated with gold nanoparticles was obtained in aptamer-aptamer sandwich type sensing format, with the limit of detection of 800. copies/ml, which is comparable to a real-time PCR method.

KW - Bovine viral diarrhea virus (BVDV)

KW - Dual aptamers

KW - SELEX

KW - SPR

KW - Sandwich

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VL - 51

SP - 324

EP - 329

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

ER -

An ultra-sensitive detection of a whole virus using dual aptamers developed by immobilization-free screening

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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